LOCUS       sequence-316634-        9501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018353
FEATURES             Location/Qualifiers
     source          1..9501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2205..2225
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2226..2249
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2275..2331
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2332..2348)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2341..2363)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2557..2576)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2676..2698
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2736..2754)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3540..4739
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4970..6118)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6390..6761)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6794..6903
                     /note="incP origin of transfer"
     rep_origin      7056..7687
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8150..8738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8639..8658
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8892..8909
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9026..9047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9062..9067,9068..9085,9086..9092)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9100..9116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9105..9127
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9124..9140
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9124..9140
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accgtgcatc
      361 agtacaagga cttcttcctt tatagcatcc ttctactgat cggcttcctc accgcggcgt
      421 gctcgcagga ctccgcgccg cccaaccccg acagctcccc cccgtccgtg gcccagggaa
      481 actacccgca caacaatagc agcccgccga cctggctcga ggcggacgag gacggccagt
      541 ggaccatgcc ggccaaaaac tacgcgtcca cgcgctacag cggcctgtcc caaatcaatc
      601 gggacaatgt caaggatctc aagctcgcct ggacgttcag caccggcctc acgccgggcc
      661 acgaggcggc cccggtgttc gtcgacgata ccctgtacgt cgtcacgccc taccccaaca
      721 ccgtgtacgc actcgcactg gacggcagca agaagtggga gtacaagccg gacacccatc
      781 aggagagcca gggcatggcc tgctgcgacg ttgtcaatcg cggcctcgtc cacgtgaacg
      841 gcaagctatt ctttaacacc ttggacaacc acgcgatcgc cctcgaagcc gacaccggca
      901 aggagctctg gaagaccaag gtgggcgacc tgcagcgggg cgaaaccatg accatggccc
      961 cgctcgtcgt caaggggaaa atgctcgtcg gaaactcggg cggcgagatg ggcgtgcgcg
     1021 gctggctgaa ggcgctggac acggagaccg gcgagctgct gtggaccgcg tactcgaccg
     1081 gcccggataa ggacgtcctg atcggcgagg agttccgccc gttctacgca caggaccagg
     1141 gcaaggacct tggggccagc tcctggcccc ccgagatgtg gaagatcggg ggcggcaccg
     1201 tgtggggctg gctgtcgtac gatccggagc tggatctcgt gtactacggc accgggaacc
     1261 cgggcccgtg gaacccggac ttccgacccg gcgacaacaa gtggaccctg accgtcttcg
     1321 ctcgccgccc cgacgacggc tccgccatct gggcctatca gatcgagcct cacgacctct
     1381 acgattacga tggggtcaac gagtcgctcc tgatcgacat gcccttcaac ggggagcagc
     1441 gcaaggtcct ggtgcgtccg gagcgcaatg gccacgtgta cgtcatggac cgcaccagcg
     1501 gcgaggtcct gagcgcgaat acctatgtcc cggtgaacgt caccgacggg gtggatctga
     1561 agaccggcca gatgcgcaag gtgaaggata agcagccggg gaccaaccgg acagtgcata
     1621 acatctgccc ggccgcccct ggcggcaagg actggcaacc gtctgcgtac tccccccgca
     1681 cggggctcat ttacatgcct cacaacaact tgtgcatgga catgcacggc acgcaggcca
     1741 actatatcgc gggtacgccc tacgtggggt ccgacgtcaa gatgttcgcc ggcccgggcg
     1801 gccaccgggg cgcctacacc gcctgggatc cggtcgcggg ctcgaaggtc tggtccatca
     1861 aggaggagtt cccggtgtgg agcggtacgg tggccaccgc gggcgacatc gtcttctatg
     1921 gcaccatgga tcgttggttc aaggcgctcg atgcgaagac tggcgagcta ctctggaagt
     1981 accgggtcgg ctcgggcatc gtcggtcagc cggtcacctt ccaagataag gacgggaagc
     2041 agtatgtcgc catcttctct ggcgtcggcg gctggtcggg cgccatcgtg tccggcggcc
     2101 tggacgcccg tgacggcacc gccgcactgg gcttcgtcaa cgcgatgaag gacctcccgc
     2161 aacataccga caagggcggc cagctctatg tcttcagctt ggccgaaaac ctctattttc
     2221 agggacatca tcaccatcac catcaccact gacagctatg accatgatta cgccaagctt
     2281 gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt cactggccgt
     2341 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     2401 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     2461 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
     2521 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     2581 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtatgct
     2641 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     2701 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     2761 ggttaatgtc atgataataa tggtttctta gcaccctttc tcggtccttc aacgttcctg
     2821 acaacgagcc tccttttcgc caatccatcg acaatcaccg cgagtccctg ctcgaacgct
     2881 gcgtccggac cggcttcgtc gaaggcgtct atcgcggccc gcaacagcgg cgagagcgga
     2941 gcctgttcaa cggtgccgcc gcgctcgccg gcatcgctgt cgccggcctg ctcctcaagc
     3001 acggccccaa cagtgaagta gctgattgtc atcagcgcat tgacggcgtc cccggccgaa
     3061 aaacccgcct cgcagaggaa gcgaagctgc gcgtcggccg tttccatctg cggtgcgccc
     3121 ggtcgcgtgc cggcatggat gcgcgcgcca tcgcggtagg cgagcagcgc ctgcctgaag
     3181 ctgcgggcat tcccgatcag aaatgagcgc cagtcgtcgt cggctctcgg caccgaatgc
     3241 gtatgattct ccgccagcat ggcttcggcc agtgcgtcga gcagcgcccg cttgttcctg
     3301 aagtgccagt aaagcgccgg ctgctgaacc cccaaccgtt ccgccagttt gcgtgtcgtc
     3361 agaccgtcta cgccgacctc gttcaacagg tccagggcgg cacggatcac tgtattcggc
     3421 tgcaactttg tcatgcttga cactttatca ctgataaaca taatatgtcc accaacttat
     3481 cagtgataaa gaatccgcgc gttcaatcgg accagcggag gctggtccgg aggccagacg
     3541 tgaaacccaa catacccctg atcgtaattc tgagcactgt cgcgctcgac gctgtcggca
     3601 tcggcctgat tatgccggtg ctgccgggcc tcctgcgcga tctggttcac tcgaacgacg
     3661 tcaccgccca ctatggcatt ctgctggcgc tgtatgcgtt ggtgcaattt gcctgcgcac
     3721 ctgtgctggg cgcgctgtcg gatcgtttcg ggcggcggcc aatcttgctc gtctcgctgg
     3781 ccggcgccac tgtcgactac gccatcatgg cgacagcgcc tttcctttgg gttctctata
     3841 tcgggcggat cgtggccggc atcaccgggg cgactggggc ggtagccggc gcttatattg
     3901 ccgatatcac tgatggcgat gagcgcgcgc ggcacttcgg cttcatgagc gcctgtttcg
     3961 ggttcgggat ggtcgcggga cctgtgctcg gtgggctgat gggcggtttc tccccccacg
     4021 ctccgttctt cgccgcggca gccttgaacg gcctcaattt cctgacgggc tgtttccttt
     4081 tgccggagtc gcacaaaggc gaacgccggc cgttacgccg ggaggctctc aacccgctcg
     4141 cttcgttccg gtgggcccgg ggcatgaccg tcgtcgccgc cctgatggcg gtcttcttca
     4201 tcatgcaact tgtcggacag gtgccggccg cgctttgggt cattttcggc gaggatcgct
     4261 ttcactggga cgcgaccacg atcggcattt cgcttgccgc atttggcatt ctgcattcac
     4321 tcgcccaggc aatgatcacc ggccctgtag ccgcccggct cggcgaaagg cgggcactca
     4381 tgctcggaat gattgccgac ggcacaggct acatcctgct tgccttcgcg acacggggat
     4441 ggatggcgtt cccgatcatg gtcctgcttg cttcgggtgg catcggaatg ccggcgctgc
     4501 aagcaatgtt gtccaggcag gtggatgagg aacgtcaggg gcagctgcaa ggctcactgg
     4561 cggcgctcac cagcctgacc tcgatcgtcg gacccctcct cttcacggcg atctatgcgg
     4621 cttctataac aacgtggaac gggtgggcat ggattgcagg cgctgccctc tacttgctct
     4681 gcctgccggc gctgcgtcgc gggctttgga gcggcgcagg gcaacgagcc gatcgctgat
     4741 cgtggaaacg ataggcctat gccatgcggg tcaaggcgac ttccggcaag ctatacgcgc
     4801 cctagaattg tcaattttaa tcctctgttt atcggcagtt cgtagagcgc gccgtgcgtc
     4861 ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc cgcttgttcc tgaaatgcca
     4921 gtaaagcgct ggctgctgaa cccccagccg gaactgaccc cacaaggccc tagcgtttgc
     4981 aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct cgcaactctt
     5041 cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca
     5101 tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg aaatagtcga
     5161 acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt
     5221 ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa cgggacgttt
     5281 tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc aggtgcccaa
     5341 cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct
     5401 tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga
     5461 aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc tgccacacca
     5521 gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg tccttgttga
     5581 cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga
     5641 acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa
     5701 tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc agcaacgcgg
     5761 cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg
     5821 tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt
     5881 cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg ggagccagtt
     5941 gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg acggactgga
     6001 aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg
     6061 aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc cgattcattc
     6121 accctccttg cgggattgcc ccgactcacg ccggggcaat gtgcccttat tcctgatttg
     6181 acccgcctgg tgccttggtg tccagataat ccaccttatc ggcaatgaag tcggtcccgt
     6241 agaccgtctg gccgtccttc tcgtacttgg tattccgaat cttgccctgc acgaatacca
     6301 gctccgcgaa gtcgctcttc ttgatggagc gcatggggac gtgcttggca atcacgcgca
     6361 ccccccggcc gttttagcgg ctaaaaaagt catggctctg ccctcgggcg gaccacgccc
     6421 atcatgacct tgccaagctc gtcctgcttc tcttcgatct tcgccagcag ggcgaggatc
     6481 gtggcatcac cgaaccgcgc cgtgcgcggg tcgtcggtga gccagagttt cagcaggccg
     6541 cccaggcggc ccaggtcgcc attgatgcgg gccagctcgc ggacgtgctc atagtccacg
     6601 acgcccgtga ttttgtagcc ctggccgacg gccagcaggt aggcctacag gctcatgccg
     6661 gccgccgccg ccttttcctc aatcgctctt cgttcgtctg gaaggcagta caccttgata
     6721 ggtgggctgc ccttcctggt tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt
     6781 acgccggcgg tagccggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc
     6841 gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg
     6901 cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct
     6961 gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac cccgaagcag
     7021 ggttatgcag cggaaaagat ccgtcgaccc tttccgacgc tcaccgggct ggttgccctc
     7081 gccgctgggc tggcggccgt ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg
     7141 tgtgcgagac accgcggccg ccggcgttgt ggatacctcg cggaaaactt ggccctcact
     7201 gacagatgag gggcggacgt tgacacttga ggggccgact cacccggcgc ggcgttgaca
     7261 gatgaggggc aggctcgatt tcggccggcg acgtggagct ggccagcctc gcaaatcggc
     7321 gaaaacgcct gattttacgc gagtttccca cagatgatgt ggacaagcct ggggataagt
     7381 gccctgcggt attgacactt gaggggcgcg actactgaca gatgaggggc gcgatccttg
     7441 acacttgagg ggcagagtgc tgacagatga ggggcgcacc tattgacatt tgaggggctg
     7501 tccacaggca gaaaatccag catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa
     7561 cctgtctttt aacctgcttt taaaccaata tttataaacc ttgtttttaa ccagggctgc
     7621 gccctgtgcg cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg aaccctcccg
     7681 gcccgctaac gcgggcctcc catcccccca ggggctgcgc ccctcggccg cgaacggcct
     7741 caccccaaaa atggcagcca agctgaccac ttctgcgctc ggcccttccg gctggctggt
     7801 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7861 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7921 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7981 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8041 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8281 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8761 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8821 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8881 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8941 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9001 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9061 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     9121 acacaggaaa cagctatgac catgattacg ccaagctagc ttcccgcttg gtcgggccgc
     9181 ttcgcgaggg cccgttgacg acaacggtgc gatgggtccc ggccccggtc aagacgatgc
     9241 caatacgttg cgacactacg ccttggcact tttagaattg ccttatcgtc ctgataagaa
     9301 atgtccgacc agctaaagac atcgcgtcca atcaaagcct agaaaatata ggcgaaggga
     9361 cgctaataag tctttcataa gaccgcgcaa atctaaaaat atccttagat tcacgatgcg
     9421 gcacttcgga tgacttccga gcgagcctgg aacctcagaa aaacgtctga gagataccgc
     9481 ggatctcaca caggaaattt c
//
