LOCUS       sequence-316637-        9507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018357
FEATURES             Location/Qualifiers
     source          1..9507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2211..2231
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2232..2255
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2281..2337
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2338..2355)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2338..2354)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2347..2369)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2563..2582)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2682..2704
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2742..2760)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3546..4745
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4976..6124)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6396..6767)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6800..6909
                     /note="incP origin of transfer"
     rep_origin      7062..7693
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8156..8744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8645..8664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8898..8915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9032..9053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9068..9073,9074..9091,9092..9098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9106..9122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9111..9133
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9130..9146
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9130..9146
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgaaga
      361 agccgctcaa gtcctggctt ctggcctcct ccgtggcggc gctgctcgcc gcgccggcgg
      421 tctcgacggc caattccgat gttgagaagc tcacccaaaa tcccgcgaac tgggccacct
      481 ggggcggcga ttacgcgggc acgcggtatt cgaagctgtc ggagatcaac gcgacaaacg
      541 tgaagaatct ccagccggcg tggtcgttca gcaccggcgt cctgcggggc cacgaggggg
      601 gccccctggt ggtcaacgac gtgctctata tccacacgcc gttcccgaac accgtctacg
      661 caatcgacca gaagacccag agcgtcatct gggagttcac gcccacccag gacgccgacg
      721 tgacgattcc cgtcatgtgc tgcgacaccg tcaaccgcgg cctcgcctac ggggacggca
      781 agatcttctt gcagcagagc gataccgtcc tgaccgccct ggacgccaag accggcaagc
      841 gcgtgtggtc cgtccagaac ggcgatccca agctcggcat gacgaatacc gcggccccgc
      901 tggtggttaa ggacaaggtc ctgaccggca ttagcggcgg cgagttcggc gtgcggggct
      961 tccttgcggc gtacgatatc aacaccggcg agctcgcctg gaagggctac tccatgggcc
     1021 cggacaaaga taccctcatc gacgcgaaga agacgacgac gtggaaggac gggaaggtga
     1081 cccctgtcgg cgagaactcc tcgctctcca cgtgggaggg cgaccagtgg aagatcggag
     1141 gcggcaccac ctggggatgg acctcgtacg acccgaagct gaacctgatc tactatggct
     1201 cggggaaccc gagcacctgg aacccggtcc agcgcccggg cgacaacaag tggtcaatgt
     1261 ccctgtgggc gcgggacgcc gataccgggg cggtcaagtg ggtctaccag atgacgccgc
     1321 acgacgagtg ggacttcgat ggcatcaacg agacggtcct cgtggaccag gacgtgaagg
     1381 gcaagccgca taagacgatc gtccatttcg accgcaatgg attcggctac accctggacc
     1441 gcgagactgg ggagctgctc gtcgcggaga agttcgataa gtcggtcaac tgggccaccc
     1501 atgtcgacat gaacacgggc cgtccgcagg tggtcgccaa gtacagcacg cagcaaaacg
     1561 gagaggatgt gaacacgaag ggcgtctgcc cggcggcgct cgggagcaag aaccaacagc
     1621 ctgtcagcta ctcgccgcag accggcctct tctacatctc gggcaatcac ctctgcatgg
     1681 attacgagcc gttcgaggtc tcgtacacgg ctggccagcc ctacgtcggc gcgacgctga
     1741 cgatgatgcc ggccggggca gacgtgctca cggggaagcc cgacaactcg accaaccttg
     1801 gccagttcac cgcgtatgac gcgaagaccg gcaagatcgc gtggtcgaac aaggagcagt
     1861 tctcggtgtg gtccggctcc gtggcgaccg ccggcggggt ggtgttctat ggcaccctcg
     1921 aaggctacct caaggccgtc gacgccaaga cgggcaagga gctgtataag ttcaagaccc
     1981 cgtcgggcat catcggaaac gtgaacacct gggaattcgg gggcaagcag tacgtgggcg
     2041 tcctgagcgg catcggcggc tgggcgggta tcggcatcgc cgcgggcctg gattcgggcg
     2101 aggagggctc caactcggag ggcctgggag ccgtcggcgc ctaccgctcg ctgtcctcct
     2161 tcaccaagct tggcggcact ctcaccgtct tcgcgctgcc gtcgaatgcc gaaaacctct
     2221 attttcaggg acatcatcac catcaccatc accactgaca gctatgacca tgattacgcc
     2281 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcact
     2341 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     2401 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     2461 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     2521 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     2581 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     2641 tatgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     2701 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     2761 tttataggtt aatgtcatga taataatggt ttcttagcac cctttctcgg tccttcaacg
     2821 ttcctgacaa cgagcctcct tttcgccaat ccatcgacaa tcaccgcgag tccctgctcg
     2881 aacgctgcgt ccggaccggc ttcgtcgaag gcgtctatcg cggcccgcaa cagcggcgag
     2941 agcggagcct gttcaacggt gccgccgcgc tcgccggcat cgctgtcgcc ggcctgctcc
     3001 tcaagcacgg ccccaacagt gaagtagctg attgtcatca gcgcattgac ggcgtccccg
     3061 gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt cggccgtttc catctgcggt
     3121 gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc ggtaggcgag cagcgcctgc
     3181 ctgaagctgc gggcattccc gatcagaaat gagcgccagt cgtcgtcggc tctcggcacc
     3241 gaatgcgtat gattctccgc cagcatggct tcggccagtg cgtcgagcag cgcccgcttg
     3301 ttcctgaagt gccagtaaag cgccggctgc tgaaccccca accgttccgc cagtttgcgt
     3361 gtcgtcagac cgtctacgcc gacctcgttc aacaggtcca gggcggcacg gatcactgta
     3421 ttcggctgca actttgtcat gcttgacact ttatcactga taaacataat atgtccacca
     3481 acttatcagt gataaagaat ccgcgcgttc aatcggacca gcggaggctg gtccggaggc
     3541 cagacgtgaa acccaacata cccctgatcg taattctgag cactgtcgcg ctcgacgctg
     3601 tcggcatcgg cctgattatg ccggtgctgc cgggcctcct gcgcgatctg gttcactcga
     3661 acgacgtcac cgcccactat ggcattctgc tggcgctgta tgcgttggtg caatttgcct
     3721 gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg gcggccaatc ttgctcgtct
     3781 cgctggccgg cgccactgtc gactacgcca tcatggcgac agcgcctttc ctttgggttc
     3841 tctatatcgg gcggatcgtg gccggcatca ccggggcgac tggggcggta gccggcgctt
     3901 atattgccga tatcactgat ggcgatgagc gcgcgcggca cttcggcttc atgagcgcct
     3961 gtttcgggtt cgggatggtc gcgggacctg tgctcggtgg gctgatgggc ggtttctccc
     4021 cccacgctcc gttcttcgcc gcggcagcct tgaacggcct caatttcctg acgggctgtt
     4081 tccttttgcc ggagtcgcac aaaggcgaac gccggccgtt acgccgggag gctctcaacc
     4141 cgctcgcttc gttccggtgg gcccggggca tgaccgtcgt cgccgccctg atggcggtct
     4201 tcttcatcat gcaacttgtc ggacaggtgc cggccgcgct ttgggtcatt ttcggcgagg
     4261 atcgctttca ctgggacgcg accacgatcg gcatttcgct tgccgcattt ggcattctgc
     4321 attcactcgc ccaggcaatg atcaccggcc ctgtagccgc ccggctcggc gaaaggcggg
     4381 cactcatgct cggaatgatt gccgacggca caggctacat cctgcttgcc ttcgcgacac
     4441 ggggatggat ggcgttcccg atcatggtcc tgcttgcttc gggtggcatc ggaatgccgg
     4501 cgctgcaagc aatgttgtcc aggcaggtgg atgaggaacg tcaggggcag ctgcaaggct
     4561 cactggcggc gctcaccagc ctgacctcga tcgtcggacc cctcctcttc acggcgatct
     4621 atgcggcttc tataacaacg tggaacgggt gggcatggat tgcaggcgct gccctctact
     4681 tgctctgcct gccggcgctg cgtcgcgggc tttggagcgg cgcagggcaa cgagccgatc
     4741 gctgatcgtg gaaacgatag gcctatgcca tgcgggtcaa ggcgacttcc ggcaagctat
     4801 acgcgcccta gaattgtcaa ttttaatcct ctgtttatcg gcagttcgta gagcgcgccg
     4861 tgcgtcccga gcgatactga gcgaagcaag tgcgtcgagc agtgcccgct tgttcctgaa
     4921 atgccagtaa agcgctggct gctgaacccc cagccggaac tgaccccaca aggccctagc
     4981 gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca
     5041 actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc
     5101 cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat
     5161 agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg
     5221 tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg
     5281 acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt
     5341 gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct
     5401 cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga
     5461 acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc
     5521 acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct
     5581 tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg
     5641 tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg
     5701 gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca
     5761 acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct
     5821 tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct
     5881 cctgttcgag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag
     5941 ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg
     6001 actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct
     6061 cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat
     6121 tcattcaccc tccttgcggg attgccccga ctcacgccgg ggcaatgtgc ccttattcct
     6181 gatttgaccc gcctggtgcc ttggtgtcca gataatccac cttatcggca atgaagtcgg
     6241 tcccgtagac cgtctggccg tccttctcgt acttggtatt ccgaatcttg ccctgcacga
     6301 ataccagctc cgcgaagtcg ctcttcttga tggagcgcat ggggacgtgc ttggcaatca
     6361 cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg gctctgccct cgggcggacc
     6421 acgcccatca tgaccttgcc aagctcgtcc tgcttctctt cgatcttcgc cagcagggcg
     6481 aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt cggtgagcca gagtttcagc
     6541 aggccgccca ggcggcccag gtcgccattg atgcgggcca gctcgcggac gtgctcatag
     6601 tccacgacgc ccgtgatttt gtagccctgg ccgacggcca gcaggtaggc ctacaggctc
     6661 atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt cgtctggaag gcagtacacc
     6721 ttgataggtg ggctgccctt cctggttggc ttggtttcat cagccatccg cttgccctca
     6781 tctgttacgc cggcggtagc cggccagcct cgcagagcag gattcccgtt gagcaccgcc
     6841 aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc tacttcacct
     6901 atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac cctttggcaa
     6961 aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat aatgaccccg
     7021 aagcagggtt atgcagcgga aaagatccgt cgaccctttc cgacgctcac cgggctggtt
     7081 gccctcgccg ctgggctggc ggccgtctat ggccctgcaa acgcgccaga aacgccgtcg
     7141 aagccgtgtg cgagacaccg cggccgccgg cgttgtggat acctcgcgga aaacttggcc
     7201 ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg
     7261 ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc agcctcgcaa
     7321 atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg
     7381 ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga
     7441 tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag
     7501 gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac
     7561 cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag
     7621 ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc
     7681 ctcccggccc gctaacgcgg gcctcccatc cccccagggg ctgcgcccct cggccgcgaa
     7741 cggcctcacc ccaaaaatgg cagccaagct gaccacttct gcgctcggcc cttccggctg
     7801 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7861 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7921 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7981 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8041 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8101 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8161 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8221 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8281 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     8341 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8401 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8461 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8521 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8581 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8641 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8701 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8761 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8821 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8881 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8941 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     9001 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     9061 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     9121 aatttcacac aggaaacagc tatgaccatg attacgccaa gctagcttcc cgcttggtcg
     9181 ggccgcttcg cgagggcccg ttgacgacaa cggtgcgatg ggtcccggcc ccggtcaaga
     9241 cgatgccaat acgttgcgac actacgcctt ggcactttta gaattgcctt atcgtcctga
     9301 taagaaatgt ccgaccagct aaagacatcg cgtccaatca aagcctagaa aatataggcg
     9361 aagggacgct aataagtctt tcataagacc gcgcaaatct aaaaatatcc ttagattcac
     9421 gatgcggcac ttcggatgac ttccgagcga gcctggaacc tcagaaaaac gtctgagaga
     9481 taccgcggat ctcacacagg aaatttc
//
