LOCUS       sequence-316638-        9510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018359
FEATURES             Location/Qualifiers
     source          1..9510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2214..2234
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2235..2258
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2284..2340
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2341..2358)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2341..2357)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2350..2372)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2566..2585)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2685..2707
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2745..2763)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3549..4748
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4979..6127)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6399..6770)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6803..6912
                     /note="incP origin of transfer"
     rep_origin      7065..7696
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8159..8747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8648..8667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8901..8918
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9035..9056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9071..9076,9077..9094,9095..9101)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9109..9125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9114..9136
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9133..9149
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9133..9149
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgaagt
      361 ttcatcgggt cgcgatggtg tgcacggcgg ttctaatggg ggccgtcggc ggaaatgcca
      421 tggccaaccc cgagaccatc cagcgccaaa aggacccgaa ccagtggccc gcgccgggcc
      481 gcgacctcgc catgacccgt cactccgccc tgaaagacat ctcaacggag aacgtgggaa
      541 agatgcagat gatctggtcg cagagctccg gcaccctccg tggccacgag ggccagccgg
      601 tcgtcgtgac cgagggcggc aaggcgatga tgtatctcat ctccggctgg cctaacattg
      661 ttcaggcgct cgacctcagc gacccggaca accccaaggc catctggaag tacgtcaaac
      721 agacggatcg ggatgagtcc gccgttccac gcgcctgctg cgacaccgtc aatcgcggtc
      781 tgagctacgc tgacgggaag gtcgtcttcg gcacgcttga cggcttcgtc atcgcgctgg
      841 acgcgaagac ggggaaggag gtgtgggtgg tcaagcacgc cttccctgag aacggtgaga
      901 ctatcacgcc ggccccgctc atcgccgaga acaaggtgct catcggcttc ggcggcgatg
      961 agttcgccgc gcgcggccgc ttcaccgcct acaacctcgc cgacggcaag aagctgtggg
     1021 agtgccatgc caccgggccg gataaggatg tgtgcgtgac caagaacacg aacaaggcga
     1081 acccgcatta cggccaaggc ggcaaggacc tcggggtgac gggctacccg ggtgacgaat
     1141 ggaagcgcgg cggcggcgcg agctggggct tctatagcta cgacccggag ctcaagctcg
     1201 tctatcacgc gacgggcaac ccgggactgt ggagcccctc ctatcggtgc aagcataaga
     1261 cgcacgagga gtgcaacgac ggctcgcagg ataaccactg gtcgatgacc atcttcgcgc
     1321 gcaaggtcga caccggcgag gccgtctggg cgtaccagat gacgcccttc gaccagtggg
     1381 attacgacgg catcaacgaa aacatgctcg ccgactggac catcgacggt aagaagcgca
     1441 aggtcctggt gcacttcgac cgcaacggtt tcgcttacgt cctcgaccgt gtcgacggca
     1501 cgctcctgaa agcgcacaag tacgtcaccg caaactgggc cgagaaagtg gacatgaaga
     1561 ccggcaagcc ggtgaaggtc aaggagcact cgccgttctc ggtggagcag aatacccaag
     1621 tgtgcccgtc cgccatgggc ggcaaggacc agcagccctg cgcgctggac ccgaatgaag
     1681 aaggagtcgc gtactgcccg accaacaact ggtgcatgga actggagccg caggagcggg
     1741 cccacacgca gcaaggcacc gtctatgtgt tcgctaacgt gtacatgtac ccggagaagc
     1801 cgggcgtcac cggcaagttc aagaagttca acgtgctgac cggcgagacc gtgtgggaga
     1861 tccccgaccc gtatcccaat tggggcggcg cccttatcac agacggtggc ctggcgttct
     1921 atggctcgct gggcggcgac ttccgcgcgg tggaccgcaa gaccggtaag gtgctctgga
     1981 gccgcaagct cggctccggc atcatcggga accccatcac gtacaaggtc aacgggaagc
     2041 agtacgtcag cgtctggtcc ggtatcggcg gctggatcgg cctcccagtt accgccggcc
     2101 tggacctcga ggacaagttc ggcgccatcg gggcgaccgc gatgaccaag gccgccaacc
     2161 tcgataagat tcctcagggg ggcaccctgt acaccttccg catcggcgac gccgaaaacc
     2221 tctattttca gggacatcat caccatcacc atcaccactg acagctatga ccatgattac
     2281 gccaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc
     2341 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     2401 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     2461 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     2521 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2581 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     2641 ttgtatgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     2701 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     2761 atttttatag gttaatgtca tgataataat ggtttcttag caccctttct cggtccttca
     2821 acgttcctga caacgagcct ccttttcgcc aatccatcga caatcaccgc gagtccctgc
     2881 tcgaacgctg cgtccggacc ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc
     2941 gagagcggag cctgttcaac ggtgccgccg cgctcgccgg catcgctgtc gccggcctgc
     3001 tcctcaagca cggccccaac agtgaagtag ctgattgtca tcagcgcatt gacggcgtcc
     3061 ccggccgaaa aacccgcctc gcagaggaag cgaagctgcg cgtcggccgt ttccatctgc
     3121 ggtgcgcccg gtcgcgtgcc ggcatggatg cgcgcgccat cgcggtaggc gagcagcgcc
     3181 tgcctgaagc tgcgggcatt cccgatcaga aatgagcgcc agtcgtcgtc ggctctcggc
     3241 accgaatgcg tatgattctc cgccagcatg gcttcggcca gtgcgtcgag cagcgcccgc
     3301 ttgttcctga agtgccagta aagcgccggc tgctgaaccc ccaaccgttc cgccagtttg
     3361 cgtgtcgtca gaccgtctac gccgacctcg ttcaacaggt ccagggcggc acggatcact
     3421 gtattcggct gcaactttgt catgcttgac actttatcac tgataaacat aatatgtcca
     3481 ccaacttatc agtgataaag aatccgcgcg ttcaatcgga ccagcggagg ctggtccgga
     3541 ggccagacgt gaaacccaac atacccctga tcgtaattct gagcactgtc gcgctcgacg
     3601 ctgtcggcat cggcctgatt atgccggtgc tgccgggcct cctgcgcgat ctggttcact
     3661 cgaacgacgt caccgcccac tatggcattc tgctggcgct gtatgcgttg gtgcaatttg
     3721 cctgcgcacc tgtgctgggc gcgctgtcgg atcgtttcgg gcggcggcca atcttgctcg
     3781 tctcgctggc cggcgccact gtcgactacg ccatcatggc gacagcgcct ttcctttggg
     3841 ttctctatat cgggcggatc gtggccggca tcaccggggc gactggggcg gtagccggcg
     3901 cttatattgc cgatatcact gatggcgatg agcgcgcgcg gcacttcggc ttcatgagcg
     3961 cctgtttcgg gttcgggatg gtcgcgggac ctgtgctcgg tgggctgatg ggcggtttct
     4021 ccccccacgc tccgttcttc gccgcggcag ccttgaacgg cctcaatttc ctgacgggct
     4081 gtttcctttt gccggagtcg cacaaaggcg aacgccggcc gttacgccgg gaggctctca
     4141 acccgctcgc ttcgttccgg tgggcccggg gcatgaccgt cgtcgccgcc ctgatggcgg
     4201 tcttcttcat catgcaactt gtcggacagg tgccggccgc gctttgggtc attttcggcg
     4261 aggatcgctt tcactgggac gcgaccacga tcggcatttc gcttgccgca tttggcattc
     4321 tgcattcact cgcccaggca atgatcaccg gccctgtagc cgcccggctc ggcgaaaggc
     4381 gggcactcat gctcggaatg attgccgacg gcacaggcta catcctgctt gccttcgcga
     4441 cacggggatg gatggcgttc ccgatcatgg tcctgcttgc ttcgggtggc atcggaatgc
     4501 cggcgctgca agcaatgttg tccaggcagg tggatgagga acgtcagggg cagctgcaag
     4561 gctcactggc ggcgctcacc agcctgacct cgatcgtcgg acccctcctc ttcacggcga
     4621 tctatgcggc ttctataaca acgtggaacg ggtgggcatg gattgcaggc gctgccctct
     4681 acttgctctg cctgccggcg ctgcgtcgcg ggctttggag cggcgcaggg caacgagccg
     4741 atcgctgatc gtggaaacga taggcctatg ccatgcgggt caaggcgact tccggcaagc
     4801 tatacgcgcc ctagaattgt caattttaat cctctgttta tcggcagttc gtagagcgcg
     4861 ccgtgcgtcc cgagcgatac tgagcgaagc aagtgcgtcg agcagtgccc gcttgttcct
     4921 gaaatgccag taaagcgctg gctgctgaac ccccagccgg aactgacccc acaaggccct
     4981 agcgtttgca atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc
     5041 gcaactcttc gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg
     5101 atccgcacat gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga
     5161 aatagtcgaa catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt
     5221 tcgtgtagtg gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac
     5281 gggacgtttt cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca
     5341 ggtgcccaac gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt
     5401 cctcggcctt cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt
     5461 agaacgtgaa ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct
     5521 gccacaccag ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt
     5581 ccttgttgac gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc
     5641 gcgtggtgaa cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc
     5701 acggcgcaat atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca
     5761 gcaacgcggc ctgcttggcc tcgctgacct gttttgccag gtcctcgccg gcggtttttc
     5821 gcttcttggt cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg
     5881 cctcctgttc gagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg
     5941 gagccagttg cacgctgtcg cgctcgatct tggccgtagc ttgctggacc atcgagccga
     6001 cggactggaa ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat
     6061 cctcggcgga aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc
     6121 gattcattca ccctccttgc gggattgccc cgactcacgc cggggcaatg tgcccttatt
     6181 cctgatttga cccgcctggt gccttggtgt ccagataatc caccttatcg gcaatgaagt
     6241 cggtcccgta gaccgtctgg ccgtccttct cgtacttggt attccgaatc ttgccctgca
     6301 cgaataccag ctccgcgaag tcgctcttct tgatggagcg catggggacg tgcttggcaa
     6361 tcacgcgcac cccccggccg ttttagcggc taaaaaagtc atggctctgc cctcgggcgg
     6421 accacgccca tcatgacctt gccaagctcg tcctgcttct cttcgatctt cgccagcagg
     6481 gcgaggatcg tggcatcacc gaaccgcgcc gtgcgcgggt cgtcggtgag ccagagtttc
     6541 agcaggccgc ccaggcggcc caggtcgcca ttgatgcggg ccagctcgcg gacgtgctca
     6601 tagtccacga cgcccgtgat tttgtagccc tggccgacgg ccagcaggta ggcctacagg
     6661 ctcatgccgg ccgccgccgc cttttcctca atcgctcttc gttcgtctgg aaggcagtac
     6721 accttgatag gtgggctgcc cttcctggtt ggcttggttt catcagccat ccgcttgccc
     6781 tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc
     6841 gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca
     6901 cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg
     6961 caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc
     7021 ccgaagcagg gttatgcagc ggaaaagatc cgtcgaccct ttccgacgct caccgggctg
     7081 gttgccctcg ccgctgggct ggcggccgtc tatggccctg caaacgcgcc agaaacgccg
     7141 tcgaagccgt gtgcgagaca ccgcggccgc cggcgttgtg gatacctcgc ggaaaacttg
     7201 gccctcactg acagatgagg ggcggacgtt gacacttgag gggccgactc acccggcgcg
     7261 gcgttgacag atgaggggca ggctcgattt cggccggcga cgtggagctg gccagcctcg
     7321 caaatcggcg aaaacgcctg attttacgcg agtttcccac agatgatgtg gacaagcctg
     7381 gggataagtg ccctgcggta ttgacacttg aggggcgcga ctactgacag atgaggggcg
     7441 cgatccttga cacttgaggg gcagagtgct gacagatgag gggcgcacct attgacattt
     7501 gaggggctgt ccacaggcag aaaatccagc atttgcaagg gtttccgccc gtttttcggc
     7561 caccgctaac ctgtctttta acctgctttt aaaccaatat ttataaacct tgtttttaac
     7621 cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg ggggtgcccc cccttctcga
     7681 accctcccgg cccgctaacg cgggcctccc atccccccag gggctgcgcc cctcggccgc
     7741 gaacggcctc accccaaaaa tggcagccaa gctgaccact tctgcgctcg gcccttccgg
     7801 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7861 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7921 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7981 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8041 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8101 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8161 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8221 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8281 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8341 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8401 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8461 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8521 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8581 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8641 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8701 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8761 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8821 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8881 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8941 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     9001 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     9061 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     9121 aacaatttca cacaggaaac agctatgacc atgattacgc caagctagct tcccgcttgg
     9181 tcgggccgct tcgcgagggc ccgttgacga caacggtgcg atgggtcccg gccccggtca
     9241 agacgatgcc aatacgttgc gacactacgc cttggcactt ttagaattgc cttatcgtcc
     9301 tgataagaaa tgtccgacca gctaaagaca tcgcgtccaa tcaaagccta gaaaatatag
     9361 gcgaagggac gctaataagt ctttcataag accgcgcaaa tctaaaaata tccttagatt
     9421 cacgatgcgg cacttcggat gacttccgag cgagcctgga acctcagaaa aacgtctgag
     9481 agataccgcg gatctcacac aggaaatttc
//
