LOCUS       sequence-316639-        8660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HA- and Clover-tagged HIF-1alpha wildtype plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8660)
  TITLE     See lab plasmids
REFERENCE   2  (bases 1 to 8660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HA-Clover-HIF-1alpha Wildtype
FEATURES             Location/Qualifiers
     source          1..8660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     865..884
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        865..883
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             906..932
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             906..932
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             912..938
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             join(945..947,948..950,951..1661)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(990..1011)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1598..1619
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      1668..1677
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4215..4233)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4216..4233)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4253..4270)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4259..4483
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4529..4957
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4616..4635)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4826..4847
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4966..4986)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4971..5300
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5151..5286
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5213..5232
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5367..6161
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5421..5440)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6031..6050
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6335..6456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6372..6391)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6426..6445
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6505..6521)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6505..6521)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6518..6540)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6529..6545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6553..6559,6560..6577,6578..6583))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6598..6619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6736..6753)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6907..7495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6987..7006)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7666..8457,8458..8526))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8289..8308
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8527..8631)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
      841 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttacca
      901 tggcctaccc ctacgacgtg cccgactacg cctccctcgg atccatggtg agcaagggcg
      961 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1021 acaagttcag cgtccgcggc gagggcgagg gcgatgccac caacggcaag ctgaccctga
     1081 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg
     1141 gctacggcgt ggcctgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     1201 agtccgccat gcccgaaggc tacgtccagg agcgcaccat ctctttcaag gacgacggta
     1261 cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1321 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1381 tcaacagcca caacgtctat atcacggccg acaagcagaa gaacggcatc aaggctaact
     1441 tcaagatccg ccacaacgtt gaggacggca gcgtgcagct cgccgaccac taccagcaga
     1501 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agccatcagt
     1561 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1621 ccgccgccgg gattacacat ggcatggacg agctgtacaa gggatccgcc accatggagg
     1681 gcgccggcgg cgcgaacgac aagaaaaaga taagttctga acgtcgaaaa gaaaagtctc
     1741 gagatgcagc cagatctcgg cgaagtaaag aatctgaagt tttttatgag cttgctcatc
     1801 agttgccact tccacataat gtgagttcgc atcttgataa ggcctctgtg atgaggctta
     1861 ccatcagcta tttgcgtgtg aggaaacttc tggatgctgg tgatttggat attgaagatg
     1921 acatgaaagc acagatgaat tgcttttatt tgaaagcctt ggatggtttt gttatggttc
     1981 tcacagatga tggtgacatg atttacattt ctgataatgt gaacaaatac atgggattaa
     2041 ctcagtttga actaactgga cacagtgtgt ttgattttac tcatccatgt gaccatgagg
     2101 aaatgagaga aatgcttaca cacagaaatg gccttgtgaa aaagggtaaa gaacaaaaca
     2161 cacagcgaag cttttttctc agaatgaagt gtaccctaac tagccgagga agaactatga
     2221 acataaagtc tgcaacatgg aaggtattgc actgcacagg ccacattcac gtatatgata
     2281 ccaacagtaa ccaacctcag tgtgggtata agaaaccacc tatgacctgc ttggtgctga
     2341 tttgtgaacc cattcctcac ccatcaaata ttgaaattcc tttagatagc aagactttcc
     2401 tcagtcgaca cagcctggat atgaaatttt cttattgtga tgaaagaatt accgaattga
     2461 tgggatatga gccagaagaa cttttaggcc gctcaattta tgaatattat catgctttgg
     2521 actctgatca tctgaccaaa actcatcatg atatgtttac taaaggacaa gtcaccacag
     2581 gacagtacag gatgcttgcc aaaagaggtg gatatgtctg ggttgaaact caagcaactg
     2641 tcatatataa caccaagaat tctcaaccac agtgcattgt atgtgtgaat tacgttgtga
     2701 gtggtattat tcagcacgac ttgattttct cccttcaaca aacagaatgt gtccttaaac
     2761 cggttgaatc ttcagatatg aaaatgactc agctattcac caaagttgaa tcagaagata
     2821 caagtagcct ctttgacaaa cttaagaagg aacctgatgc tttaactttg ctggccccag
     2881 ccgctggaga cacaatcata tctttagatt ttggcagcaa cgacacagaa actgatgacc
     2941 agcaacttga ggaagtacca ttatataatg atgtaatgct cccctcaccc aacgaaaaat
     3001 tacagaatat aaatttggca atgtctccat tacccaccgc tgaaacgcca aagccacttc
     3061 gaagtagtgc tgaccctgca ctcaatcaag aagttgcatt aaaattagaa ccaaatccag
     3121 agtcactgga actttctttt accatgcccc agattcagga tcagacacct agtccttccg
     3181 atggaagcac tagacaaagt tcacctgagc ctaatagtcc cagtgaatat tgtttttatg
     3241 tggatagtga tatggtcaat gaattcaagt tggaattggt agaaaaactt tttgctgaag
     3301 acacagaagc aaagaaccca ttttctactc aggacacaga tttagacttg gagatgttag
     3361 ctccctatat cccaatggat gatgacttcc agttacgttc cttcgatcag ttgtcaccat
     3421 tagaaagcag ttccgcaagc cctgaaagcg caagtcctca aagcacagtt acagtattcc
     3481 agcagactca aatacaagaa cctactgcta atgccaccac taccactgcc accactgatg
     3541 aattaaaaac agtgacaaaa gaccgtatgg aagacattaa aatattgatt gcatctccat
     3601 ctcctaccca catacataaa gaaactacta gtgccacatc atcaccatat agagatactc
     3661 aaagtcggac agcctcacca aacagagcag gaaaaggagt catagaacag acagaaaaat
     3721 ctcatccaag aagccctaac gtgttatctg tcgctttgag tcaaagaact acagttcctg
     3781 aggaagaact aaatccaaag atactagctt tgcagaatgc tcagagaaag cgaaaaatgg
     3841 aacatgatgg ttcacttttt caagcagtag gaattggaac attattacag cagccagacg
     3901 atcatgcagc tactacatca ctttcttgga aacgtgtaaa aggatgcaaa tctagtgaac
     3961 agaatggaat ggagcaaaag acaattattt taataccctc tgatttagca tgtagactgc
     4021 tggggcaatc aatggatgaa agtggattac cacagctgac cagttatgat tgtgaagtta
     4081 atgctcctat acaaggcagc agaaacctac tgcagggtga agaattactc agagctttgg
     4141 atcaagttaa ctgacaattc tgcagatatc catcacactg gcggccgctc gagcatgcat
     4201 ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact
     4261 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     4321 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     4381 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     4441 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     4501 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     4561 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4621 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4681 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4741 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4801 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4861 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4921 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     4981 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5041 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5101 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     5161 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5221 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5281 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     5341 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     5401 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     5461 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     5521 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     5581 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     5641 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     5701 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     5761 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     5821 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     5881 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     5941 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     6001 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     6061 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     6121 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     6181 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     6241 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     6301 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     6361 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6421 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     6481 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6541 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6601 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6661 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6721 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6781 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6841 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6901 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6961 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7021 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7081 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7141 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7201 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7261 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7321 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7381 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7441 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7501 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7561 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7621 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7681 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7741 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7801 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7861 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7921 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7981 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8041 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8101 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8161 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8221 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8281 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8341 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8401 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8461 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8521 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8581 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8641 ccgaaaagtg ccacctgacg
//
