LOCUS       sequence-316676-         500 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within 
            the protein kinase domain of both ABL1 isoforms 1a and 1b..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Beetham H, Griffith BGC, Murina O, Loftus AE, Parry DA, Temps C, 
            Culley J, Muir M, Unciti-Broceta A, Sims AH, Byron A, Brunton VG
  TITLE     Loss of Integrin-linked kinase sensitizes breast cancer to SRC 
            inhibitors.
  JOURNAL   Cancer Res. 2021 Dec 17. pii: 0008-5472.CAN-21-0373. doi: 
            10.1158/0008-5472.CAN-21-0373.
  PUBMED    34921014
REFERENCE   2  (bases 1 to 500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2021 Dec 17. pii: 0008-5472.CAN-21-0373. doi: 
            10.1158/0008-5472.CAN-21-0373."
COMMENT     Sequence Label: eSpCas9(1.1)-ABL1-gRNA1-exon4
FEATURES             Location/Qualifiers
     source          1..500
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        160..235
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
       61 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
      121 gtggaaagga cgaaacaccg ggggacacac catagacagg ttttagagct agaaatagca
      181 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
      241 tgttttagag ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc
      301 aattctgcag acaaatggct ctagaggtac ccgttacata acttacggta aatggcccgc
      361 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg
//
