LOCUS       sequence-31668-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, 
            McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA
  TITLE     Measuring mechanical tension across vinculin reveals regulation of 
            focal adhesion dynamics.
  JOURNAL   Nature. 2010 Jul 8. 466(7303):263-6.
  PUBMED    20613844
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jul 8. 466(7303):263-6."
COMMENT     Sequence Label: TS module
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             148..855
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta ccgagctcgg
      121 atccactagt ccagtgtggt ggaattcatg gtgagcaagg gcgaggagac cacaatgggc
      181 gtaatcaagc ccgacatgaa gatcaagctg aagatggagg gcaacgtgaa tggccacgcc
      241 ttcgtgatcg agggcgaggg cgagggcaag ccctacgacg gcaccaacac catcaacctg
      301 gaggtgaagg agggagcccc cctgcccttc tcctacgaca ttctgaccac cgcgttcgcc
      361 tacggcaaca gggccttcac caagtacccc gacgacatcc ccaactactt caagcagtcc
      421 ttccccgagg gctactcttg ggagcgcacc atgaccttcg aggacaaggg catcgtgaag
      481 gtgaagtccg acatctccat ggaggaggac tccttcatct acgagataca cctcaagggc
      541 gagaacttcc cccccaacgg ccccgtgatg cagaagaaga ccaccggctg ggacgcctcc
      601 accgagagga tgtacgtgcg cgacggcgtg ctgaagggcg acgtcaagca caagctgctg
      661 ctggagggcg gcggccacca ccgcgttgac ttcaagacca tctacagggc caagaaggcg
      721 gtgaagctgc ccgactatca ctttgtggac caccgcatcg agatcctgaa ccacgacaag
      781 gactacaaca aggtgaccgt ttacgagagc gccgtggccc gcaactccac cgacggcatg
      841 gacgagctgt acaaggggcc aggtggtgca gggccaggtg gtgc
//
