LOCUS       sequence-316680-         425 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within 
            the protein kinase domain of both ABL1 isoforms 1a and 1b..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Beetham H, Griffith BGC, Murina O, Loftus AE, Parry DA, Temps C, 
            Culley J, Muir M, Unciti-Broceta A, Sims AH, Byron A, Brunton VG
  TITLE     Loss of Integrin-linked kinase sensitizes breast cancer to SRC 
            inhibitors.
  JOURNAL   Cancer Res. 2021 Dec 17. pii: 0008-5472.CAN-21-0373. doi: 
            10.1158/0008-5472.CAN-21-0373.
  PUBMED    34921014
REFERENCE   2  (bases 1 to 425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2021 Dec 17. pii: 0008-5472.CAN-21-0373. doi: 
            10.1158/0008-5472.CAN-21-0373."
COMMENT     Sequence Label: eSpCas9(1.1)-ABL1-gRNA2-exon4
FEATURES             Location/Qualifiers
     source          1..425
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     108..127
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        206..281
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
       61 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
      121 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      181 acaccgaaga aatacagcct gacgggtttt agagctagaa atagcaagtt aaaataaggc
      241 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt ttagagctag
      301 aaatagcaag ttaaaataag gctagtccgt ttttagcgcg tgcgccaatt ctgcagacaa
      361 atggctctag aggtacccgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      421 aacga
//
