LOCUS       sequence-316701-       10295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bioluminescent repair reporter (BLRR) for DNA double strand break 
            (DSB) repairs: homology-directed repair (HDR) and non-homologous end
            joining (NHEJ)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10295)
  AUTHORS   Chien JC, Tabet E, Pinkham K, da Hora CC, Chang JC, Lin S, Badr CE, 
            Lai CP
  TITLE     A multiplexed bioluminescent reporter for sensitive and non-invasive
            tracking of DNA double strand break repair dynamics in vitro and in 
            vivo.
  JOURNAL   Nucleic Acids Res. 2020 Aug 14. pii: 5892751. doi: 
            10.1093/nar/gkaa669.
  PUBMED    32797168
REFERENCE   2  (bases 1 to 10295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 14. pii: 5892751. doi: 10.1093/nar/gkaa669."
COMMENT     Sequence Label: pLenti-BLRR
FEATURES             Location/Qualifiers
     source          1..10295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1941..2244
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2245..2448
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2399..2419
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2445..2469
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2553..2577
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3173..3226
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(4900..4924)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4955..5543
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5008..5028)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5426..5437)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5557..6056
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5592..5615)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5989..6008
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6077..6676
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6077..6096)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6573..6593
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6814..7047
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7119..7240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7156..7175)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7210..7229
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7280..7415
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7435..7454)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7436..7454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7464..7481)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7464..7480)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7473..7495)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7622..8077
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7709..7728)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7919..7940
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8103..8207
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8208..8276,8277..9068)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8426..8445)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9239..9827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9728..9747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9981..9998
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10115..10136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10151..10156,10157..10174,10175..10181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10189..10205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10194..10216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10213..10229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10213..10229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10248..10268
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10250..10268
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     1981 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2041 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2101 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2161 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2221 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2281 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2341 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2401 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2461 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc
     2521 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctcca
     2581 ccatgggagt caaagttctg tttgccctga tctgcatcgc tgtggccgag gccaagccca
     2641 ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag caacttcgcg accacggatc
     2701 tcgatgctga ccgcgggaag ttgcccggca agaagctgcc gctggaggtg ctcaaagaga
     2761 tggaagccaa tgcccggaaa gctggctgca ccaggggctg tctgatctgc ctgtcccaca
     2821 tcaagtgcac gcccaagatg aagaagttca tcccaggacg ctgccacacc tacgaaggcg
     2881 acaaagagtc cgcagatcga attaccctgt tatccctaca cctataacat ctcgcgatcg
     2941 tcgacattcc cgagattcct gggttcaagg acttggagcc catggagcag ttcatcgcac
     3001 aggtcgatct gtgtgtggac tgcacaactg gctgcctcaa agggcttgcc aacgtgcagt
     3061 gttccgacct gctcaagaag tggctgccgc aacgctgtgc gacctttgcc agcaagatcc
     3121 agggccaggt ggacaagatc aagggggccg gtggcgacta gcttattccg gtgagggcag
     3181 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccg ggccctatga agataattat
     3241 cctttctgtg attctggctt actgtgttac agtgaattgt caggatgcat gtccagtaga
     3301 ggcggaaccg ccatcttcta ccccgaccgt accaacctcc tgcgaagcta aagaagggga
     3361 gtgcatcgat acaaggtgcg ctacctgcaa acgggatatc ctgtccgacg gactttgcga
     3421 aaataaaccc gggaagacct gctgtcgaat gtgtcagtat gtcatcgaat gccgggtcga
     3481 ggccgccggt tattttagaa cattttacgg taaacggttt aatttccagg aacccggcaa
     3541 atacgtactg gctcgcggca ccaagggtgg cgactggagc gtcaccctga caatggaaaa
     3601 cctggacggg cagaaaggag ccgtgcttac taaaactacc ctggaggtgg cgggagacgt
     3661 aattgacatc actcaggcaa cggctgaccc aataaccgtg aacggaggag ctgatcccgt
     3721 gattgcaaac cctttcacta ttggcgaggt cacgattgcc gtcgtcgaaa ttccaggctt
     3781 caacatcaca gtgatcgagt tcttcaagct gatcgtcatt gatatcctcg gcggacggtc
     3841 cgttcgcatc gcacctgaca cagccaacaa gggcctgatc tctggcattt gtggtaactt
     3901 ggaaatgaat gatgctgatg actttacaac ggacgccgac caactggcta ttcaacctaa
     3961 tatcaacaaa gagtttgatg gatgtccctt ttacggaaat ccttcagaca tcgaatactg
     4021 caaaggcctc atggaaccgt accgggccgt ttgcagaaat aacatcaact tctactatta
     4081 tactctgagc tgcgcatttg catactgtat gggcggtgag gagagagcca aacatgtgct
     4141 tttcgactat gtggagacct gcgccgcccc ggagactcgc ggtacctgcg tcctgagcgg
     4201 ccataccttc tatgacacct tcgataaggc taggtaccag ttccaagggc cttgcaaaga
     4261 gctcctgatg gccgcagatt gttactggaa cacttgggac gtcaaagttt cccatcggga
     4321 cgtagagagc tacacggaag ttgagaaggt gaccatcagg aagcagagta ccgtcgtaga
     4381 cctgatcgtc gacggcaagc aggtaaaggt aggaggcgtg gacgttagta ttccgtattc
     4441 ttctgaaaat acgagcatct actggcagga tggagacatt ctgacaaccg ccatccttcc
     4501 agaagctctg gtggtgaagt ttaacttcaa gcagctgctg gtagtgcaca ttcgcgaccc
     4561 attcgacggg aaaacctgtg ggatttgcgg caactacaac caggactcaa ctgacgattt
     4621 ctttgacgcc gaaggggctt gcgctcttac cccaaatccg cctggatgca ccgaagagca
     4681 aaagcctgaa gcggaacggc tgtgcaattc actgtttgat tcttcaatag atgagaaatg
     4741 caacgtgtgt tacaaacctg accgcatcgc acgctgcatg tatgagtatt gcctgagagg
     4801 tcaacaaggg ttctgcgatc acgcgtggga atttaagaaa gaatgctaca taaagcacgg
     4861 ggatacattg gaggtgccgc cagaatgcca gtagtctaga cccagctttc ttgtacaaag
     4921 tggttgatat ccagcacagt ggcggccgct cgacaatcaa cctctggatt acaaaatttg
     4981 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5041 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     5101 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     5161 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5221 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     5281 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     5341 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     5401 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     5461 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     5521 ctccctttgg gccgcctccc cgcctggaat tctaccgggt aggggaggcg cttttcccaa
     5581 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     5641 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     5701 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     5761 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     5821 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     5881 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     5941 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     6001 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     6061 gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     6121 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     6181 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     6241 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     6301 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     6361 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     6421 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     6481 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     6541 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     6601 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     6661 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
     6721 accccatgca tctcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag
     6781 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac tcccaacgaa
     6841 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6901 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6961 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7021 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     7081 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     7141 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7201 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     7261 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7321 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7381 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     7441 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     7501 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     7561 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     7621 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7681 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7741 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7801 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7861 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7921 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7981 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8041 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     8101 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8161 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8221 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8281 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8341 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8401 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8461 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8521 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8581 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8641 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8701 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8761 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8821 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8881 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8941 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9001 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9061 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9121 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9181 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9241 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9301 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9361 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9421 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9481 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9541 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9601 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9661 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9721 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9781 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9841 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9901 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9961 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    10021 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    10081 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    10141 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    10201 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    10261 actaaaggga acaaaagctg gagctgcaag cttaa
//
