LOCUS       sequence-31671-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, 
            McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA
  TITLE     Measuring mechanical tension across vinculin reveals regulation of 
            focal adhesion dynamics.
  JOURNAL   Nature. 2010 Jul 8. 466(7303):263-6.
  PUBMED    20613844
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jul 8. 466(7303):263-6."
COMMENT     Sequence Label: Tailless VinculinTS
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(62..79)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    68..292
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      338..766
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(425..444)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     635..656
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(775..795)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 acgagctgta caagtaggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca
       61 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
      121 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
      181 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
      241 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
      301 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
      361 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      421 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      481 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      541 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      601 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
      661 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
      721 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
      781 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      841 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
      901 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
      961 tcccgnccct aactccgccc agttccnccc attc
//
