LOCUS       sequence-316716-        6059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 610, Emission: 652.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6059)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mMaroon-Cx43-7
FEATURES             Location/Qualifiers
     source          1..6059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        84..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     542..562
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2008..2017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2014..2748
                     /codon_start=1
                     /product="bright, rapidly maturing, monomeric far-red 
                     fluorescent protein (Bajar et al., 2016)"
                     /label=mMaroon1
                     /translation="MVSKGEELIKENMHTKLYLTGTVNNHYFECTAEGEGKPYEGTQTN
                     RIKVVRGGPLPFAFDILAPCFMYGSKTFINHPPDIPDYFKQSFPEGFTWERTTVYEDGG
                     TLTATQDTSLQDGCLIYNVQVRGENFPSNGPVMQKKTLGWEASTETLYPADGSLEGRLD
                     WALKLVGGGHLHCRLETTYRSKKPAKNLKMPGVYFIDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     polyA_signal    2870..2991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2907..2926)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2961..2980
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2998..3453)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3135..3156)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3347..3366
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3480..3584
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3586..3943
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3586..3777
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3609..3629)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3794..3929
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3978..4772
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3978..4772
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4032..4051)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4642..4661
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4960..4979)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5004..5051
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5380..5968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5869..5888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataaccgtat taccgccatg cattagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
      601 aaccgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattcggc
      661 agcacttttc tttcattggg gggaaggcgt gaggaaagta ccaaacagca gcagactttt
      721 aaactttaaa cagacaggtc tgagagcctg aactctcatt tttcctttga cttcagcctc
      781 caaggagttc caccaacttt ggcgcgccgg cttcactttc attaagtgaa agagaggtgc
      841 ccagacatgg gtgactggag tgccttgggg aagcttctgg acaaggtcca agcctactcc
      901 accgctggag ggaaggtgtg gctgtcagtg ctcttcatat tcagaatcct gctcctgggg
      961 acagctgttg agtcagcttg gggtgatgaa cagtctgcct ttcgctgtaa cactcaacaa
     1021 cctggctgcg aaaacgtctg ctatgacaag tccttcccca tctctcacgt gcgcttctgg
     1081 gtccttcaga tcatattcgt gtctgtgccc acactcctgt acttggccca tgtgttctat
     1141 gtgatgagga aggaagagaa gctaaacaag aaagaagagg agctcaaagt ggcccagact
     1201 gacggggtca acgtggagat gcacctgaag cagattgaaa tcaagaagtt caagtacggg
     1261 attgaagagc acggcaaggt gaaaatgagg ggcggcttgc tgagaaccta catcatcagc
     1321 atcctcttca agtctgtctt cgaggtggcc ttcctgctca tccagtggta catctatggg
     1381 ttcagcttga gcgcggtcta cacctgcaag agagatccct gcccccacca ggtagactgc
     1441 ttcctctcac gtcccacgga gaaaaccatc ttcatcatct tcatgctggt ggtgtccttg
     1501 gtgtctctcg ctttgaacat cattgagctc ttctacgtct tcttcaaggg cgttaaggat
     1561 cgcgtgaagg gaagaagcga tccttaccac gccaccactg gcccactgag cccatcaaaa
     1621 gactgcggat ctccaaaata cgcctacttc aatggctgct cctcaccaac ggctccactc
     1681 tcgcctatgt ctcctcctgg gtacaagctg gttactggtg acagaaacaa ttcctcgtgc
     1741 cgcaattaca acaagcaagc tagcgagcaa aactgggcga actacagcgc agagcaaaat
     1801 cgcatggggc aggccggaag caccatctcc aactcgcacg cccagccgtt cgatttcccc
     1861 gacgacaacc agaatgccaa aaaagttgct gctggacatg aactccagcc attagccatc
     1921 gtggaccaac gaccttccag cagagccagc agccgcgcca gcagcaggcc tcggcctgat
     1981 gacctggaga ttgcggatcc accggtcgcc accatggtga gcaagggcga ggagctgatc
     2041 aaggagaaca tgcacaccaa gctgtacctg acaggcaccg tgaacaacca ctacttcgaa
     2101 tgcaccgccg aaggggaggg caagccctac gagggcaccc agaccaacag gatcaaggtg
     2161 gtgcgcggag gccccctgcc gttcgcattc gacatcctgg ccccctgctt tatgtacggg
     2221 agcaagacct tcattaacca tccacctgac attcccgatt attttaagca gtccttccct
     2281 gagggcttca catgggagag aaccaccgtt tacgaagacg ggggcacact cactgctaca
     2341 caggacacca gcctccagga cggctgcctg atctacaacg tccaagtcag aggggagaac
     2401 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc cagtaccgag
     2461 accctgtacc ccgctgacgg cagcctggaa ggcagactgg actgggccct gaagctcgtg
     2521 ggcgggggcc acctgcactg cagactggaa accacataca gatccaagaa acccgcaaag
     2581 aacctcaaga tgcccggcgt ctattttatt gacaggagac tggaaagaat caaggaggcc
     2641 gacaatgaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
     2701 agcaaactgg ggcacaaact taatggcatg gacgagctgt acaagtaagc ggccgcgact
     2761 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     2821 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     2881 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2941 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
     3001 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     3061 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     3121 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     3181 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     3241 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     3301 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     3361 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     3421 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     3481 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3541 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     3601 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     3661 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     3721 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     3781 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3841 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     3901 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     3961 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     4021 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     4081 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     4141 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     4201 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     4261 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     4321 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     4381 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     4441 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     4501 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     4561 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     4621 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     4681 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     4741 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     4801 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     4861 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     4921 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     4981 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     5041 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     5101 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     5161 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     5221 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5281 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5341 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5401 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5461 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5521 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5581 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5641 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5701 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5761 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5821 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5881 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5941 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6001 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgg
//
