LOCUS       sequence-31672-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, 
            McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA
  TITLE     Measuring mechanical tension across vinculin reveals regulation of 
            focal adhesion dynamics.
  JOURNAL   Nature. 2010 Jul 8. 466(7303):263-6.
  PUBMED    20613844
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jul 8. 466(7303):263-6."
COMMENT     Sequence Label: VinculinTS
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(142..849)
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gctctcgccc ttgctcacca tagcaccacc tggacctgca ccacctggac ctgcaccacc
       61 tggacctgca ccaccgggcc ctgcaccacc tggccctgca ccacctggcc ctgcaccacc
      121 tggccctgca ccacctggcc ccttgtacag ctcgtccatg ccgtcggtgg agttgcgggc
      181 cacggcgctc tcgtaaacgg tcaccttgtt gtagtccttg tcgtggttca ggatctcgat
      241 gcggtggtcc acaaagtgat agtcgggcag cttcaccgcc ttcttggccc tgtagatggt
      301 cttgaagtca acgcggtggt ggccgccgcc ctccagcagc agcttgtgct tgacgtcgcc
      361 cttcagcacg ccgtcgcgca cgtacatcct ctcggtggag gcgtcccagc cggtggtctt
      421 cttctgcatc acggggccgt tgggggggaa gttctcgccc ttgaggtgta tctcgtagat
      481 gaaggagtcc tcctccatgg agatgtcgga cttcaccttc acgatgccct tgtcctcgaa
      541 ggtcatggtg cgctcccaag agtagccctc ggggaaggac tgcttgaagt agttggggat
      601 gtcgtcgggg tacttggtga aggccctgtt gccgtaggcg aacgcggtgg tcagaatgtc
      661 gtaggagaag ggcagggggg ctccctcctt cacctccagg ttgatggtgt tggtgccgtc
      721 gtagggcttg ccctcgccct cgccctcgat cacgaaggcg tggccattca cgttgccctc
      781 catcttcagc ttgatcttca tgtcgggctt gattacgccc attgtggtct cctcgccctt
      841 gctcaccatc tcgacctcat c
//
