LOCUS       sequence-316752-       14788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS 
            Reagent Program Cat #11051) and Q63A,Q67A mutation in capsid (CA 
            Q63A,Q67A)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14788)
  AUTHORS   Christensen DE, Ganser-Pornillos BK, Johnson JS, Pornillos O, 
            Sundquist WI
  TITLE     Reconstitution and visualization of HIV-1 capsid-dependent 
            replication and integration in vitro.
  JOURNAL   Science. 2020 Oct 9;370(6513). pii: 370/6513/eabc8420. doi: 
            10.1126/science.abc8420.
  PUBMED    33033190
REFERENCE   2  (bases 1 to 14788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2020-10-9"; volume: "370(6513). pii: 370/6513/eabc8420. doi";
            pages: " 10.1126/science.abc8420"
COMMENT     Sequence Label: SG3-Delta-ENV CA Q63A,Q67A
FEATURES             Location/Qualifiers
     source          1..14788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             69..77
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(357..376)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(358..376)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(395..411)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(395..411)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(408..430)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    419..435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(443..449,450..467,468..473))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    488..509
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(626..643)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(797..1385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(877..896)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1556..2347,2348..2416))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2179..2198
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2417..2521)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2548..3003
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2635..2654)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2845..2866
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3129..3151
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3143..3160
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3144..3160
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             5303..5559
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5606..5731
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     CDS             7010..10021
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSSTRRELQVWGGDNNSPSEAG
                     AGRQGNVSLSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQIPIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYSGIKVKQLCKLLRGTKALTEVVTLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARTRGAHT
                     NDVKQLTEAVQKITTECIIIWGKTPKFRLPIQKETWETWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIEGAETFYVDGAANRETKLGKAGYVTNKGRQKVVTLTDTTNQKTELE
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGVRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKIILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTIHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVVESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDNKDPLWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    9706..9823
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     RBS             11613..11624
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    12706..12939
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13124..13168
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     LTR             14400..14656
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgctgctgct gaaatgctaa gatgaaattt atagccttaa gtctatgcat tggaaaagaa
       61 agctttttta aggaggtaga aaaggaatag gaaatcaaac ctaaagacag tagaagtaaa
      121 gaaataatga agacaagagc agaaattagt gaatagaaag taatcttaca tagagaaaat
      181 tgacaaagct aagaaaaagt tggttctttg aaaagattaa tgtaattgac cacttagcaa
      241 aactgataaa aggaggatgg ggagggggag agagaacata aatttaaata ataggaattg
      301 aaaaatgtca catcactacg gatcctctag agtcgacctg caggcatgca agctttccct
      361 atagtgagtc gtattagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      421 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      481 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      541 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      601 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      661 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      721 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      781 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      841 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      901 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      961 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1021 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1081 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1141 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1201 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1261 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1321 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1381 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1441 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1501 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1561 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1621 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1681 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1741 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1801 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1861 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1921 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1981 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2041 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2101 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2161 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2221 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2281 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2341 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2401 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2461 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2521 gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg
     2581 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     2641 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     2701 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     2761 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     2821 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     2881 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     2941 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     3001 tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     3061 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     3121 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattccttc tatggaatac
     3181 tttttaaata tatagtctga taatatataa ctgacacgtt ttctgttgtt tcataatggt
     3241 tgattttctc agggctacgt ttctctattt tttcctgttc tcaaatctta actctccatc
     3301 tctgtcctca agtggtaagt gatcttgtgt cttattttga aattaaagtc atcaacaaat
     3361 tatttcaact catagattta tagttgtgga agacttacta ttcaaatgga attagaacgt
     3421 tttgggggat aggatgcagt agttcttggt agtccaagac tcttaataaa acaactagaa
     3481 aagccaggta atttacaaaa tttatatatt tagcgataga gaacaatggg tgtaaaaatg
     3541 attaaagaaa gcaaaatttt gcaccctggg ggaatacctt tcacaagtga gctgagaggt
     3601 ctgcagctga tcattgtttt tcctgggtac agagtagagg aggcctttgg gctgcagcct
     3661 gagtggcaga ttggaaatat gaggggcctt taaacacaga gaacgccctc agtatattcc
     3721 taaattctgt agttcagctg ggcggaagag ataaaccaaa atctcagaaa aggagattgg
     3781 gatattttct agtcctatgg tgttgagata aagattctct aggggaagcg acttgagaac
     3841 aataggtgag agttgaaagc gtttgaggaa gtggtgaata cacactgatg attttcaaca
     3901 cacccagttt cttcccccgt agacatttgc caaattctga agctgcttag gctagagagc
     3961 taaaccagaa gtctcttaaa aacaaagcaa ttcttatagt atctaggtac ctagaagata
     4021 acgatctgct agcttttgaa atgaaggctc tgcagagtct catcctagga acagggacaa
     4081 accagaggta gtctccttca tagcaaaact gctactcagc agtgttctat gattctggca
     4141 ttatagagga agtagtaaaa gtatatgtat gaaaattgtg ttaagtgaag gatgaataag
     4201 aaaatatcta gggtaaccac taaaagaaaa gaatgtgtaa caaatgaata cagaaaaaaa
     4261 ttggatatag aatatgtgat aaattcagaa gaagacaaga aagaaaatat aaaacaagca
     4321 ggcaactaaa ttagtaatta tgttacatgg aaatgggcta aatatttcaa gtaaatggca
     4381 aaaatcatca gactggacaa ataataatta cctaaatata agtacctaga aagattgaga
     4441 gaaaatgatg cgaaaaggta taccatccaa atgctaactg aaggaaataa gtgtggctat
     4501 accaatcagg caaaggagac tttaatacaa taaacagtag tagaaaaaga gaactacatt
     4561 ttatagtgta aggggattga tccaccagag ttaccacaat cctttatgtg tacccagtaa
     4621 aatagcccaa acatctataa accaaaaatt ttattttgaa ctaaaagaaa cagacaaata
     4681 tgccctcaca atggatgcac atatatagga catacataca tcacaattag caggcttgat
     4741 ctaattgtca tacgtagaac attgaaacta ttaatggtgg tattaaccat cttttccctt
     4801 gcacatggaa attgtactag aattgaccat aagctagggc catgaacaaa gcctcagcaa
     4861 atatcattga ttttaataat tgaatatgtt gtttgtatta tagtaaataa aaataactag
     4921 aaaattggaa gggctaatta tctcccaaag aagacaagat atccttgatc tgtggaccta
     4981 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     5041 tccactgacc tttggatggt gtttcaagct agtaccagtt gatccagaga agatagaaga
     5101 ggccaatgaa ggagaaaaca acagcttgct acaccctatg agccagcatg ggatggatga
     5161 cccggagaga gaagtgctag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     5221 ccgagagctg catccggagt actacaagaa ccgatgacat cgcgctttct acaagggact
     5281 ttccgctggg gactttccag gggaggcgtg gcctgggcgg gaccggggag tggcgagccc
     5341 tcagatgctg catataagca gctgcttttg cctgtactgg gtctctctgg ttagaccaga
     5401 tctgagcctg ggagctctct ggctagctag ggaacccact gcttaagcct caataaagct
     5461 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt agctagagat
     5521 ccctcatacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggaccg
     5581 gaaagcgaaa gagaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     5641 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     5701 ctagaaggag agagatgggt gcgagagcgt cagtattgag cgggggaaaa ttggataagt
     5761 gggagaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catctagtat
     5821 gggcaagcag ggagctagaa cgattcgcag ttaatcccgg cctgttagaa acagcagaag
     5881 gctgtagaca aatactggga cagctacaac cgtcccttca gacaggatca gaagaactta
     5941 aatcattata taatacaata gcagtcctct attgtgtgca tcaaatgata gatgtaaaag
     6001 acaccaagga agctttagag aagatagagg aagagcaaaa caacagtaag aaaaaagcac
     6061 agcaagcagc agctgacaca ggaaacagca gccaggtcag ccgaaattac cctatagtgc
     6121 agaacatcca ggggcaaatg gtacatcagg ccatatcacc cagaacttta aatgcatggg
     6181 taaaagtagt agaagagaag gctttcagcc cagaagtaat acccatgttt tcagcattat
     6241 cagaaggagc caccccacaa gatttaaaca ccatgctaaa cacagtgggg ggacatgcag
     6301 cagctatggc aatgttaaaa gagaccatca atgaggaagc tgcagaatgg gatagattgc
     6361 atccagtgca agcagggcct gttgcaccag gccagatgag agaaccaagg ggaagtgaca
     6421 tagcaggaac tactagtacc cttcaggaac aaataggatg gatgacaaat aatccaccta
     6481 tcccagtagg agaaatctat aaaagatgga taatcctggg attaaataaa atagtaagga
     6541 tgtatagtcc ttctagcatt ctggacataa gacaaggacc aaaggaatcc tttagagact
     6601 atgtagacag gttctataaa actttaagag ccgagcaagc ttcacaggag gtaaaaaatt
     6661 ggatgacaga aaccttgttg gtccaaaatg cgaacccaga ttgtaagacc attttaaaag
     6721 cattgggacc aggagctaca ctagaggaaa tgatgacagc atgtcaggga gtgggaggac
     6781 ccggccacaa agcaagagtt ttggcggaag caatgagcca agtaacaaat tcagccacca
     6841 taatgatgca gagaggcaat tttaggaacc aaagaaagac tgttaagtgt ttcaattgtg
     6901 gcaaagaagg gcatatagcc agaaattgca aggcccctag gaaaaggggc tgctggaaat
     6961 gtggaaagga aggacaccaa atgaaagatt gtacagagag acaggctaat tttttaggga
     7021 agatctggcc ttcccacaag ggaaggccag ggaattttcc tcagagcaga ctagagccaa
     7081 cagctccacc agaagagagc ttcaggtttg gggaggagac aacaactccc cctcagaagc
     7141 aggagcggga agacaaggaa atgtatccct tagcttccct cagatcactc tttggcaacg
     7201 acccctcgtc acaataaaga taggggggca actaaaggag gctctattag atacaggagc
     7261 agatgataca gtattagaag aaatgaattt gccagggaga tggaagccaa aaatgatagg
     7321 gggaattgga ggttttatca aagtaagaca gtatgatcag atacccatag aaatctgtgg
     7381 acataaagct ataggtacag tattagtagg acctacacct gtcaacataa ttggaagaaa
     7441 tctgttgact cagattggtt gcactctaaa ttttcccatt agtcctattg aaactgtacc
     7501 agtaaaatta aagccaggaa tggatggccc aaaagttaaa caatggccat tgacagaaga
     7561 aaaaataaaa gcattagtag aaatttgtac agaaatggaa aaggaaggga agatttcaaa
     7621 aattgggcct gaaaatccat acaatactcc agtatttgct ataaagaaaa aagacagtac
     7681 taaatggaga aaattagtag atttcagaga acttaataag aggactcaag acttctggga
     7741 agttcaatta ggaataccac atcccgctgg attaaaaaag aaaaaatcag taacagtact
     7801 agatgtgggt gatgcatatt tctcagttcc cttagataaa gacttcagga aatatactgc
     7861 atttaccata cctagtataa acaatgagac accagggatt agatatcagt acaatgtgct
     7921 cccacaggga tggaaaggat caccagcaat attccaaagt agcatgacaa aaatcttaga
     7981 gccttttaga aagcaaaatc cagacatagt tatctatcag tacatggatg atttgtatgt
     8041 aggatctgac ttagaaatag ggcagcatag aacaaaaata gaggaactga gacaacatct
     8101 gttaaggtgg ggatttacca caccagacaa aaaacatcag aaagaacctc cattcctttg
     8161 gatgggttat gaactccatc ctgataaatg gacagtacag cctatagtgc tgccagaaaa
     8221 agacagctgg actgtcaatg acatacagaa gttagtggga aaattgaatt gggcaagtca
     8281 gatttactca gggatcaaag tgaagcagtt atgtaaactc cttaggggaa ccaaagcact
     8341 aacagaagta gtaacactaa cagaagaagc agagctagaa ctggcagaaa acagggaaat
     8401 tctaaaagaa ccagtacatg gagtgtatta tgacccatca aaagacttaa tagcagaaat
     8461 acagaaacag gggcaaggcc aatggacata tcaaatttat caagagccat ttaaaaatct
     8521 gaaaacagga aaatatgcaa gaacgagggg tgcccacact aatgatgtaa aacaattaac
     8581 agaggcagtg caaaaaataa ccacagaatg cataataata tggggaaaaa ctcctaaatt
     8641 tagactgccc atacaaaaag aaacatggga aacatggtgg acagagtatt ggcaagccac
     8701 ctggattcct gaatgggagt ttgtcaatac ccctccctta gtgaaattat ggtaccagtt
     8761 agagaaagag cccatagaag gcgcagaaac tttctatgta gatggagcag ctaacaggga
     8821 gactaaatta ggaaaagcag gatatgttac taacaaagga agacaaaaag ttgtcaccct
     8881 aactgacaca acaaatcaga agactgagtt agaagcaatt catctagctt tgcaggattc
     8941 tggattagaa gtaaacatag taacagactc acaatatgca ttaggaatca ttcaagcaca
     9001 accagataaa agtgaatcag aattagtcag tcaaataata gagcagttaa taaaaaagga
     9061 aaaggtctac ctggcatggg taccagcaca caaaggaatt ggaggaaatg aacaagtaga
     9121 taaattagtc agtgctgggg tcaggaaagt actattttta gatggaatag ataaggccca
     9181 agaagaacat gagaaatatc acagtaattg gagagccatg gctagtgatt ttaacttacc
     9241 acctgtagta gcaaaagaaa tagtagccag ctgtgataaa tgtcagctaa aaggagaagc
     9301 catgcatgga caagtagact gtagtccagg aatatggcaa ctagattgca cacatctaga
     9361 aggaaaaatt atcctggtgg cggttcatgt agccagtgga tatatagaag cagaagttat
     9421 tccagcagag acagggcagg aaacagcata ctttctctta aaattagcag gaagatggcc
     9481 agtaaaaaca atacatacag acaatggcag caatttcacc agtaccacgg ttaaggccgc
     9541 ctgttggtgg gcagggatca agcaggaatt tggcattccc tacaatcccc aaagtcaagg
     9601 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     9661 tgaacatctt aaaacagcag tacaaatggc agtatttatc cacaatttta aaagaaaagg
     9721 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     9781 aactaaagaa ctacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     9841 caacaaagat ccactttgga aaggaccagc aaagcttctc tggaaaggtg aaggggcagt
     9901 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     9961 agattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
    10021 gaacatggat aagtttagta aaacaccata tgtatatttc aaggaaagca aagggatggt
    10081 tttatagaca tcactatgaa agcactcacc caaaaataag ttcagaagta cacatcccac
    10141 taggggatgc tagattggta ataacaacat attggggtct gcatacagga gaaagagatt
    10201 ggcatttggg tcatggagtc tccgtagaat ggaggaaaaa gagatatagc acacaagtag
    10261 accctgacct agcagaccaa ctaattcatc tgtattactt tgattgtttt tcagaatctg
    10321 ccataagaaa tgccatatta ggacatatag ttagtcctag gtgtgaatat caagcaggac
    10381 ataacaaggt aggatctcta cagtacctag cactagcagc attaataaca ccaaaaagga
    10441 taaagccacc tttgcctagt gttacaaaac taacagagga tagatggaac aagccccaga
    10501 agaccaaggg ccacagaggg agccatacaa tgaatggaca ctagagcttt tagaagaact
    10561 taagaatgaa gctgttagac attttcctag gatatggctc catggcttag ggcaatatat
    10621 ctatgaaact tatggggata cttgggcagg agtggaagcc ctagtaagaa ctctgcaaca
    10681 actgctgttt actcttttca gaattgggtg tcgacatagc agaataggca ttactcaacg
    10741 aagaagagca agaaatggag ccagtagatc ctagactaga gccctggaag catccaggaa
    10801 gccagcctaa aactgcttgt accaaatgct attgtaaaaa gtgttgctta cattgccaag
    10861 tttgtttcat gacaaaaggc ttaggcatct cctatggcag gaagaagcgg agacagcgac
    10921 gaagagctcc tcaagacagt caggctcatc aagcttctct atcaaagcag taagtagtgc
    10981 atgtaatgca acctatacaa atagcagcaa tagtagcatt agtagtggta ggaataatag
    11041 caatagttgt gtggtccata gtattaatag aatataggaa aatattaaga caaagaaaaa
    11101 tagacaggtt aattaaaaga ataagtaaaa gagcagaaga cagtggcaat gagagtgaag
    11161 gagatcagga agaattatca gcacttgtgg agatggggca ccatgctctt tgggatattg
    11221 atgatctata gtgctgcagg gaatttgtgg gtcacagtat attatggggt acctgtatgg
    11281 aaagaagcaa ccaccactct attttgtgca tcagatgcta aagcatatga tacagaggta
    11341 cataatgttt gggccacaca tgcctgtata cccacagacc ccaacccaca agagatagta
    11401 ttagaaaatg tgacagaaga ttttaacatg tggaaaaata acatggtaga acagatgcag
    11461 gaggatataa tcagtttatg ggatcaaagc ctaaagccat gtgtaaaatt aaccccactc
    11521 tgtgttactc taaattgcac tgatgagttg aagaatgcta ctaataccac tagctagtac
    11581 taatacccct agtggtagct ggaaaaagat ggaaagagga gaaataaaga actgctcttt
    11641 caatgtctta ggagataaga agcagaaagc atatgcactc ttttataaac ttgatgtagt
    11701 accaatagat aatgataaga atagtaccaa atataggttg ataagttgta acacctcagt
    11761 cactacacag gcctgtccaa agatatcctt tgagccaatt cccatacatt attgtgcccc
    11821 ggctggtttt gcgattctaa agtgtaaaga taagaagttc aatggaacag gatcatgtaa
    11881 aaatgtcagc acagtacaat gtacacatgg aattaagcca gtagtatcaa ctcaactgct
    11941 gttaaatggc agcctagcag aagaagaggt agtaattaga tctgccaatt tctcggacaa
    12001 tgctaaaacc ataatagtac agctgaaaga agctgtagaa attaattgca caagacccaa
    12061 caaaaaaaca agaaaacgta taactacggg accagggaga gtatattata caacaggaga
    12121 aatagtagga gatataagac aagcacattg taaccttagt agagcaaaat ggaatgacac
    12181 tttaagacag atagttataa aattaagaga acaatttgag aataaaacaa tagtctttaa
    12241 tcaatcctca ggaggggacc cagaaattgt aatgcatagt tttaattgtg gaggggaatt
    12301 tttctactgt aagtcaacac aactgtttaa tagtacttgg gctgggaata atacttggaa
    12361 tagtagtgct gaaaggtcag atgacactgg aggaaatatc acactcccat gcagaataaa
    12421 acaaattata aacatgtggc aggaagtagg aaaagcaatg tatgcccctc ccatcagcgg
    12481 acaaattaga tgtacatcaa atattacagg gctgctatta acaagagatg gtggtacgag
    12541 cgataccaat accaccgaga ccttcagacc tggaggagga gatatgaggg acaattggag
    12601 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccac
    12661 ggcaaagaga agagtggtgc aaagagaaaa aagagcagtg ggaataggag ctctgttcct
    12721 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgt tgacggtaca
    12781 ggccagacaa ttattgtctg gtatagtgca acagcagaac aatttgctga gggctattga
    12841 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagagt
    12901 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
    12961 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgaa
    13021 tcagatttgg gataacatga cttggatgca gtgggaaaga gaaattgaaa attacacaga
    13081 cttaatatac aacttaattg aagaatcgca gaaccagcaa gaaaagaatg aacaagaatt
    13141 attggaatta gataaatggg caagtttgtg gaattggttt acaataacaa actggctgtg
    13201 gtatataaaa atattcataa tgatagtagg aggcttgata ggtttaagaa tagtttttac
    13261 tgtactttct atagtgaata gagttaggca gggatactca ccattgtcgt ttcagaccca
    13321 cctcccaacc ccgaggggac ccgacaggcc cgaaggaatc gaagaagaag gtggagagag
    13381 agacagagac agatccggtc gattagtgaa cggattctta gcacttttct gggacgatct
    13441 gcggagcctg tgcctcttca gctaccaccg cttgagagac ttaatcttgg ttgtaacgag
    13501 gattgtggaa cttctgggac gcagggggtg ggaagccctc aagtattggt ggagtctcct
    13561 acagtattgg agccaggaac taaagaatag tgctgttaac ttgcttaatg tcacagccat
    13621 agcagtagct gagggaacag atagggttat agaagtagta caaagaactt atagagctat
    13681 tctccacata cctagaagaa taagacaggg cttggaaagg cttttgctat aagatgggtg
    13741 gcaagtggtc aaaacgtatg gagggtggat ggcatgctgt aagggaaaga atgagacgag
    13801 ctgagccagc agcagatggg gtgggagcag tatctagaga cctggaaaaa catggagcaa
    13861 tcacaagtag caatacagca gctaacaatg ctgattgtgc ctggctggaa gcacaagagg
    13921 aggaggaggt gggttttcca gtcagacctc aggtaccttt aagaccaata acttacaagg
    13981 cagctgtaga tattagccac tttttaaaag aaaagggggg actggaaggg ctaattttct
    14041 cccaaagaag acaagatatc cttgatctgt ggacctacca cacacaaggc tacttccctg
    14101 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgtt
    14161 tcaagctagt accagttgat ccagagaaga tagaagaggc caatgaagga gaaaacaaca
    14221 gcttgctaca ccctatgagc cagcatggga tggatgaccc ggagagagaa gtgctagagt
    14281 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggagtact
    14341 acaagaaccg atgacatcgc gctttctaca agggactttc cgctggggac tttccagggg
    14401 aggcgtggcc tgggcgggac cggggagtgg cgagccctca gatgctgcat ataagcagct
    14461 gcttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
    14521 tagctaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
    14581 tgtgcccgtc tgttgtgtga ctctggtagc tagagatccc tcataccctt ttagtcagtg
    14641 tggaaaatct ctagcaaaaa ttcctagctg cctagaaata aaacactatg cttttgaata
    14701 acatatgact taaaagaaga atttatgatg gctattagaa aatattttaa actgattgaa
    14761 aatgaaggtg aactacaatg atctagat
//
