LOCUS       sequence-31676-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Gavet O, Pines J
  TITLE     Progressive activation of CyclinB1-Cdk1 coordinates entry to 
            mitosis.
  JOURNAL   Dev Cell. 2010 Apr 20. 18(4):533-43.
  PUBMED    20412769
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2010 Apr 20. 18(4):533-43."
COMMENT     Sequence Label: inactive cdk1 FRET sensor (2328)
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(140..163)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(164..415)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(206..227)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     555..574
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     813..834
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
       61 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagttatc
      121 tagatccggt ggatccttac ttatcgtcgt catccttgta atccttgtac agctcgtcca
      181 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
      241 cgttggggtc tttgctcagc ttggactggg tgctcaggta gtggttgtcg ggcagcagca
      301 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
      361 cctcgatgtt gtggcggatc ttgaagttgg ccttgatgcc gttcttctgc ttgtcggcgg
      421 tgatatagac gttgtcgctg atggcgttgt actccagctt gtgccccagg atgttgccgt
      481 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
      541 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
      601 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
      661 gggcgaagca ctgcacgccc cangtcaggg tggtcacgag ggtgggccag ggcacgggca
      721 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtagtggcat cgccctcgcc
      781 ctcgccggan acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccagga
//
