LOCUS       sequence-31677-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Gavet O, Pines J
  TITLE     Progressive activation of CyclinB1-Cdk1 coordinates entry to 
            mitosis.
  JOURNAL   Dev Cell. 2010 Apr 20. 18(4):533-43.
  PUBMED    20412769
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2010 Apr 20. 18(4):533-43."
COMMENT     Sequence Label: inactive cdk1 FRET sensor (2328)
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(92..94,95..97,98..808)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(398..417)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tctctggcta ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagcttgcta gcgctaccgg tatggtgagc aaaggcgaag agctgttcac
      121 cggcgtggtg cccatcctgg tggagctgga cggcgacgtg aacggccaca agttcagcgt
      181 gagcggcgag ggcgagggcg acgccaccta cggcaagctg accctgaagc tgctgtgcac
      241 caccggcaag ctgcccgtgc cctggcccac cctggtgacc accctgggct acggcgtgca
      301 gtgcttcgcc cggtaccccg accacatgaa gcagcacgac ttcttcaaga gcgccatgcc
      361 cgagggctac gtgcaggagc ggaccatctt cttcaaggac gacggcaact acaagacccg
      421 ggccgaggtg aagttcgagg gcgacaccct ggtgaaccgg atcgagctga agggcatcga
      481 cttcaaggag gacggcaaca tcctgggcca caagctggag tacaactaca acagccacaa
      541 cgtgtacatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccggca
      601 caacatcgag gacggcggcg tgcagctggc cgaccactac cagcagaaca cccccatcgg
      661 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagagcg ccctgttcaa
      721 ggaccccaac gagaagcggg accacatggt gctgctggag ttcctgaccg ccgccggcat
      781 caccgagggc atgaacgagc tctataagag atctcacctc agtgacatgc tgcagcagct
      841 gcacagtgtc aatgcctcca agccctcgg
//
