LOCUS       sequence-31678-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Gavet O, Pines J
  TITLE     Progressive activation of CyclinB1-Cdk1 coordinates entry to 
            mitosis.
  JOURNAL   Dev Cell. 2010 Apr 20. 18(4):533-43.
  PUBMED    20412769
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2010 Apr 20. 18(4):533-43."
COMMENT     Sequence Label: cdk1 FRET sensor (2327)
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(136..159)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(join(160..870,871..873,874..876))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(202..223)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     551..570
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     810..831
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca gttatctaga
      121 tccggtggat ccttacttat cgtcgtcatc cttgtaatcc ttgtacagct cgtccatgcc
      181 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
      241 ggggtctttg ctcagcttgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg
      301 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc
      361 gatgttgtgg cggatcttga agttggcctt gatgccgttc ttctgcttgt cggcggtgat
      421 atagacgttg tcgctgatgg cgttgtactc cagcttgtgc cccaggatgt tgccgtcctc
      481 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
      541 ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta
      601 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc
      661 gaagcactgc acgccccagg tcagggtggt cacgagggtg ggccagggca cgggcagctt
      721 gccggtggtg cagatgaact tcagggtcag cttgccgtan gtggcatcgc cctcgccctc
      781 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
      841 caccccggtg aacagctcct cgcccttgct caccatctcg agtgtttcca ttgggcttgg
      901 agaggaagta tcaaccaaaa t
//
