LOCUS       sequence-31679-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Gavet O, Pines J
  TITLE     Progressive activation of CyclinB1-Cdk1 coordinates entry to 
            mitosis.
  JOURNAL   Dev Cell. 2010 Apr 20. 18(4):533-43.
  PUBMED    20412769
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2010 Apr 20. 18(4):533-43."
COMMENT     Sequence Label: cdk1 FRET sensor (2327)
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(83..85,86..88,89..799)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(389..408)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgct agcgctaccg gtatggtgag caaaggcgaa gagctgttca ccggcgtggt
      121 gcccatcctg gtggagctgg acggcgacgt gaacggccac aagttcagcg tgagcggcga
      181 gggcgagggc gacgccacct acggcaagct gaccctgaag ctgctgtgca ccaccggcaa
      241 gctgcccgtg ccctggccca ccctggtgac caccctgggc tacggcgtgc agtgcttcgc
      301 ccggtacccc gaccacatga agcagcacga cttcttcaag agcgccatgc ccgagggcta
      361 cgtgcaggag cggaccatct tcttcaagga cgacggcaac tacaagaccc gggccgaggt
      421 gaagttcgag ggcgacaccc tggtgaaccg gatcgagctg aagggcatcg acttcaagga
      481 ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca acgtgtacat
      541 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccggc acaacatcga
      601 ggacggcggc gtgcagctgg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      661 cgtgctgctg cccgacaacc actacctgag ctaccagagc gccctgttca aggaccccaa
      721 cgagaagcgg gaccacatgg tgctgctgga gttcctgacc gccgccggca tcaccgaggg
      781 catgaacgag ctctataaga gatctcacct cagtgacatg ctgcagcagc tgcacagtgt
      841 caatgcctcc aagccctcgg
//
