LOCUS       sequence-316795-        3585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dihydroxyacetone synthase (DHAS) promoter module for episomal P. 
            pastoris promoter shuffling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Pullmann P, Weissenborn MJ
  TITLE     Improving the Heterologous Production of Fungal Peroxygenases 
            through an Episomal Pichia pastoris Promoter and Signal Peptide 
            Shuffling System.
  JOURNAL   ACS Synth Biol. 2021 Jun 2. doi: 10.1021/acssynbio.0c00641.
  PUBMED    34075757
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Jun 2. doi: 10.1021/acssynbio.0c00641."
COMMENT     Sequence Label: pAGM9121_pDHAS
FEATURES             Location/Qualifiers
     source          1..3585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             285..1076
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1417..2005
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1906..1925
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgccgaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
       61 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
      121 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
      181 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
      241 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg gaagcggtga
      301 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
      361 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
      421 gcgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
      481 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
      541 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
      601 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
      661 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
      721 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
      781 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
      841 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
      901 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
      961 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     1021 tggaagaatt tgtccattac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
     1081 tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
     1141 tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct tttattattt
     1201 ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgctgt cagaccaagt
     1261 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1321 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1381 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1441 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1501 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1561 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1621 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1681 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1741 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1801 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1861 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1921 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1981 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctgca
     2041 ctctgtggtc tcaaacgctc gagagccgtg gaacagagtc ccctgaatct cttgccaagc
     2101 ggcttgctgc tgcatctgcg gagatggagt acgccagggc agtggacacg acaaggtcat
     2161 tgtcaacgat gaccttgaga aggcgtactc tgagctgaag gagttcattt tcgccgagcc
     2221 catctaagca ttcataaatt tttaatatct agagctctca tacgggacag tatctcctcc
     2281 aaccttgcgt caagcttgtc ctcttcatgc tcctcaacag tcatggcatc cagctgctgc
     2341 tgcttttgct ccagcctggc atatatgtcg ccatacagct tgagttggat tttgatgaaa
     2401 ctctcaaagg tagggtccac cagtgacagt cgcagcgcaa tgaactgctc gatttcgttc
     2461 ttgagccgtg tgttgatgtc cgtgtagata ttttctgcct cgtcgtactc aactttgaac
     2521 ttctgcagct tgtccaggct cttctgtaac tggtctgttt tctcggtgtg atgctgctcg
     2581 gtcacctgtc gctcaatcgc ttcgtactcg ctctgcagct tcgaaacctt gaatcgtgaa
     2641 acgtcgtaat ccaccttttt gcgtgcgcgc ttcttgatca gcttgttgat ctcgtcgttg
     2701 tacttcttca gctcgttaat cggctccacg accgtgatgc tcattggctc cagaatttct
     2761 ggcagaatat tgtctttgat gtctaccacc atctgcagat aattcagaga aataccatct
     2821 ctggggttca ccttgtgctc ttctggccgt tccgcagctt ccgaccgctt atcagccttg
     2881 agctcaaagc tatagtctcc gtaaaacgag tccagtgttc tagccatatt tatctgagtc
     2941 tcgagcagat tctccgaaat tgcccacaaa acggcctagt tcctggtcca gctcgttggt
     3001 gtaagtctcg agtttgcgga aattggcctc ctggacgtca aactcaggat caacagaggg
     3061 ctcacctttg tttgtgcgta gtatcacatg tgctccggca cgattgacag cttttttaaa
     3121 cccaacccat gacatgtcga ggaaagggtc gtttcgggga gttaaatatt tttggctatg
     3181 tagcagacat gtttcgacgc tggcgtcgcg tcgatcggaa aatattaccc caggaacaag
     3241 cacttgcttg ggttagccac caccctgcgc aagccttttt gccggctcta cacagggcca
     3301 atgaaatctg ggcggaatct gaaaccgatg aaacggacga cactggcaac aagctcactg
     3361 cactatttta tttttctagt gaaatagcct atcctcgtct cgctcccctc atacctgtaa
     3421 aggggtgcaa tttagcctcg ttccagccat tcacgggcca ctcaacaaca cgtcggctac
     3481 catggggtgc ttgggcacca aaaggcctat aaataggccc ccatccgtct gctacacagt
     3541 catctctgtc ttttcttccc atcatacaaa atgcgagacc acgaa
//
