LOCUS       sequence-316806-       10208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transposon-based SARS-CoV-2 secretable trimeric Spike stable 
            expression vector for protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Arias-Arias JL, Molina-Castro SE, Monturiol-Gross L, Lomonte B, 
            Corrales-Aguilar E
  TITLE     Stable production of recombinant SARS-CoV-2 receptor-binding domain 
            in mammalian cells with co-expression of a fluorescent reporter and 
            its validation as antigenic target for COVID-19 serology testing.
  JOURNAL   Biotechnol Rep (Amst). 2023 Mar;37:e00780. doi: 
            10.1016/j.btre.2022.e00780. Epub 2022 Dec 31.
  PUBMED    36619904
REFERENCE   2  (bases 1 to 10208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Rep (Amst)."; date: "2023-03"; volume: "37:e00780. doi"; pages: " 
            10.1016/j.btre.2022.e00780. Epub 2022 Dec 31"
COMMENT     Sequence Label: pSBbi-RP-CoV2/stSpike-puro
FEATURES             Location/Qualifiers
     source          1..10208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1130..1150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1208..1217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4862..4879
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5000..5017
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5040..5057)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5046..5270
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5702..5806
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5807..5875,5876..6667)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6025..6044)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6838..7426
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7327..7346
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7580..7597
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7971..7990
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    7989..8044
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     8090..8109
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(8209..8808)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8292..8312)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8789..8808
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(8809..8865)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(8875..9576)
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MLSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(8958..8981)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(9156..9175)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9425..9443
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        join(10208,1..1182)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
       61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
      961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1021 atggagtttc cccatactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1141 acagtggttc aaagtttttt tcttccattt aaggtgtcgt gaaaactacc ccaagctggc
     1201 ctctgaggcc accatgggct tcgtgtttct ggtgctgctg cctctggtgt ctagccaggc
     1261 cgcttgtgtg aacctgacca ccaggaccca gctgcctcct gcctacacca atagcttcac
     1321 ccgaggagtt tactaccccg ataaggtgtt ccgaagcagc gtgctgcaca gcacacagga
     1381 cctgtttctg ccattcttca gtaacgtcac ctggttccac gccatccacg tgtccggcac
     1441 aaatggcaca aagagattcg acaaccccgt gctgcctttc aacgacggcg tgtacttcgc
     1501 ctccacggag aagagcaaca tcatccgggg ctggatcttc ggcaccactc tcgacagcaa
     1561 aacacagagc ctgctcatcg ttaacaacgc cacaaacgtg gtgattaagg tgtgtgaatt
     1621 ccagttttgt aatgatcctt ttctgggcgt gtactaccac aagaacaaca agtcctggat
     1681 ggaatctgag ttcagggtgt acagcagcgc caacaactgc actttcgagt acgtgtcaca
     1741 gcccttcctg atggacctgg aaggaaagca aggcaatttc aagaatctgc gggaattcgt
     1801 gttcaaaaac atcgacggct acttcaagat ctacagcaag cacaccccta tcaacctggt
     1861 gcgggacctg cctcagggct ttagcgcttt ggagcctctg gttgatctgc ctatcggtat
     1921 caacatcacc agattccaaa ccctgctggc cctgcacaga tcttacctga caccaggcga
     1981 cagcagcagc ggatggaccg ccggagccgc cgcttattac gtgggctacc tgcagcctag
     2041 aacctttctg ctgaaatata atgaaaacgg caccatcacc gatgccgtgg actgcgccct
     2101 ggaccctctg agcgagacaa agtgtaccct taagagcttc acagttgaga agggcatcta
     2161 ccagacatct aacttccggg tccagcctac agagtccatc gttagattcc ccaacatcac
     2221 caacctgtgt cctttcggag aagttttcaa cgctacccgc tttgcctctg tatacgcctg
     2281 gaatcggaag agaatctcca actgcgtggc cgattactct gtgctgtata actctgcctc
     2341 cttcagcaca ttcaagtgct acggagtcag ccctaccaag ctgaacgacc tgtgctttac
     2401 caacgtgtac gccgactcgt tcgtgattag aggcgatgag gtgagacaga tcgcccctgg
     2461 ccagaccggc aagatcgctg actacaacta caagctgcct gacgacttta ccggctgcgt
     2521 gatcgcctgg aacagcaaca atctcgactc taaggtgggc ggcaactaca actacctgta
     2581 cagactgttc cgtaagagca acctcaagcc tttcgagcgg gacatcagca ccgaaatcta
     2641 ccaggccggc agtaccccgt gcaacggcgt ggaaggcttc aattgctact tccccctcca
     2701 gagctacggc ttccagccca ccaatggcgt cggctaccag ccgtaccggg tcgtggtgct
     2761 gagcttcgaa ctgctgcacg ctcctgccac cgtgtgcggt cctaagaaaa gcaccaacct
     2821 ggtcaagaac aaatgcgtga acttcaactt caacgggctg acaggcaccg gcgtactgac
     2881 agaaagcaac aaaaagttcc tgcccttcca gcagttcggc agagacatcg ccgacaccac
     2941 cgatgccgtg cgggaccccc agaccctgga aatcctggac atcacccctt gcagctttgg
     3001 cggagtttcc gtgatcaccc ccggaacaaa caccagcaat caggtggccg tgctttacca
     3061 ggacgtgaac tgtaccgagg tgccagtggc tatccacgct gatcagctga cccccacctg
     3121 gcgggtgtac agcaccggct ctaatgtgtt ccagacgaga gccggatgtc tgatcggcgc
     3181 tgagcacgtg aacaacagct acgagtgtga cattcctata ggagccggca tctgcgccag
     3241 ctaccagacc cagacaaact ctcctgcctc cgtggcctcc cagtctatca tcgcctacac
     3301 catgtcactg ggcgccgaga atagcgtggc ctacagcaac aacagtatcg ccattccaac
     3361 caactttact atcagcgtga ccacggagat cctgcccgtg agcatgacca agacaagcgt
     3421 ggattgcaca atgtacattt gcggagacag caccgaatgc tctaacttac tgctgcagta
     3481 cggctccttc tgcacccagc tcaaccgagc tctgaccggc atcgcagtgg aacaggataa
     3541 gaacacccag gaggtgttcg ctcaggtgaa gcagatctac aagacccctc ctatcaagga
     3601 cttcggagga ttcaacttca gccagatcct gcccgaccca agcaagccca gcaaacggag
     3661 cttcattgaa gacctgctgt tcaacaaggt gaccctggct gatgccggct tcatcaagca
     3721 atacggcgat tgcctgggcg acatcgccgc cagagacctg atctgtgccc agaagttcaa
     3781 cgggcttaca gtactccctc ctctgctgac agatgagatg atcgcccagt acacaagtgc
     3841 cctgttagca gggaccatca cctctggatg gacattcggc gccggcgccg ctctgcagat
     3901 cccctttgcc atgcagatgg cctatagatt taacggcatc ggcgtgacac agaacgtgct
     3961 gtacgagaac cagaagctga tcgccaacca gttcaattcc gcaatcggaa aaatccagga
     4021 cagcctgtcc agcacggcct cagctctggg gaaactgcaa gatgtcgtga atcagaacgc
     4081 ccaagccctg aacaccctgg tgaagcaact gagctcgaat tttggcgcca tctctagcgt
     4141 gctgaatgac atcctgagca gactggaccc tcctgaggcc gaggtgcaga tcgacagact
     4201 aatcactgga agactgcaga gcctgcagac ctacgtgaca caacagctga tcagagccgc
     4261 cgaaatcaga gcctccgcca atctggccgc caccaagatg agcgagtgcg tgctgggcca
     4321 aagcaagcgg gtggacttct gtggcaaggg ctaccacctg atgagctttc ctcaaagcgc
     4381 cccccacggc gtggtgttcc tgcacgtgac ctacgtgcct gctcaagaaa aaaacttcac
     4441 cacagcccct gctatatgtc acgacggcaa ggcccacttc ccaagagagg gcgtctttgt
     4501 gagtaacggc acacactggt tcgtaaccca aagaaacttc tacgaacccc aaatcatcac
     4561 aacagataac acgttcgtgt ctggcaactg cgacgtggtc atcggcatcg tgaacaatac
     4621 cgtctacgac cctctgcagc ccgagctgga ctcatttaag gaggaactgg acaagtattt
     4681 caagaaccac acctcccctg atgtggacct gggcgacatc agcggcatta acgcctccgt
     4741 ggtgaacatc cagaaagaga ttgatagact gaacgaagtg gccaagaacc tgaacgagag
     4801 cctgatcgac ctgcaagagc tgggcaagta tgagcagtac attaaatggc ctagcggtag
     4861 actggtccct agaggcagcg gcagcggcag tggcagcggc tatatccctg aagcccctag
     4921 agatggccag tgctacgtgc ggtgcgatgg agagtgggtg ctgctgagca cattcctggg
     4981 cagcggtagc ggcagcggcc atcatcacca tcaccactga tctagagctc gctgatcagc
     5041 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     5101 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     5161 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     5221 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     5281 ggaaagaacc agctgggcct actagatagc ggacccctta ccgaaacatc gccgcattct
     5341 gcagaggagt cgaacttagt gtatgtaaac ttctgaccca ctggaattgt gatacagtga
     5401 attataagtg aaataatctg tctgtaaaca attgttggaa aaatgacttg tgtcatgcac
     5461 aaagtagatg tcctaactga cttgccaaaa ctattgtttg ttaacaagaa atttgtggag
     5521 tagttgaaaa acgagtttta atgactccaa cttaagtgta tgtaaacttc cgacttcaac
     5581 tgtataggga tccaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt
     5641 ctccttacgc atctgtgcgg tatttcacac cgcatcaggt ggcacttttc ggggaaatgt
     5701 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5761 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5821 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5881 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5941 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6001 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6061 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6121 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6181 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6241 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6301 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6361 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6421 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6481 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6541 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6601 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6661 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6721 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6781 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6841 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6901 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6961 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7021 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7081 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7141 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7201 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7261 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7321 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7381 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7441 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7501 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7561 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggatccct atacagttga
     7621 agtcggaagt ttacatacac cttagccaaa tacatttaaa ctcacttttt cacaattcct
     7681 gacatttaat cctagtaaaa attccctgtc ttaggtcagt taggatcacc actttatttt
     7741 aagaatgtga aatatcagaa taatagtaga gagaatgatt catttcagct tttatttctt
     7801 tcatcacatt cccagtgggt cagaagttta catacactcg actcctctgc agaatgcggc
     7861 gatgtttcgg taaggggtcc cgagtagcta gttcatggca gccagcatat ggcatatgtt
     7921 gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct cccatatgtc
     7981 cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat aaatttcctt
     8041 tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt tttggcagag
     8101 ggaaaaagat ctcagtggta tttgtgagcc agggcattgg ccacaccagc caccaccttc
     8161 tgataggcag cctgcacctg aggagtgaat tcacgacagg ccttcgaatc aggcaccggg
     8221 cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt
     8281 gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg
     8341 caccccggcg cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt
     8401 gccctggtgg tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg
     8461 gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc
     8521 ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt
     8581 ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac
     8641 gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac
     8701 ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg
     8761 gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg ggccaggatt
     8821 ctcctcgacg tcaccagcct gcttcagcag gctgaagtta gtagctccac tgcccttgta
     8881 cagctcgtcc atgccgtaca ggaacaggtg gtggcggccc tcggagcgct cgtactgttc
     8941 cacgatggtg tagtcctcgt tgtgggaggt gatgtccagc ttggtgtcca cgtagtagta
     9001 gccgggcagt tgcacgggct tcttggccat gtagatggtc ttgaactcca ccaggtagtg
     9061 gccgccgtcc ttcagcttca gggcctggtg gatctcgccc ttcagcacgc cgtcgcgggg
     9121 gtacaggcgc tcggtggagg cctcccagcc cattgtcttc ttctgcatta cggggccgtc
     9181 gggggggaag ttggtgccgc gcatcttcac cttgtagatc agcgtgccgt cctgcaggga
     9241 ggagtcctgg gtcacggtca ccagaccgcc gtcctcgaag ttcatcacgc gctcccactt
     9301 gaagccctcg gggaaggaca gcttcttgta atcggggatg tcggcggggt gcttcacgta
     9361 cgccttggag ccgtacatga actgggggga caggatgtcc caggcgaagg gcagggggcc
     9421 gcccttggtc accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc
     9481 gccctcgatc tcgaactcat ggccgttcat ggagccctcc atgcgcacct tgaagcgcat
     9541 gaactctttg atgacctcct cgcccttgct caacatggtg gcctcaggtg cagaggtttc
     9601 tacagggaaa gagagaaagg ctacagttag tttgctgtga tgatgctaag tgccgaggtc
     9661 agatgtctat ggattgtaca cggaatccca acgcaggaac caaaaaacac tcacccaaaa
     9721 aaatggcgct aaggccgaga gaaaggcgaa gttccgtcta cggctatata ccttgaaaca
     9781 ggggcggcag aagtgacgca acgcggttgc gcggacggct tcctgtgttt tcattggtcg
     9841 aagcgttctg cgcgtgcgca gagattcccc agggaaggcg ggccctcttc cgaatgtcca
     9901 atccggacat tcttccggtt ggacctccgc ggtcagcgct gtgcccggcg agagatcacg
     9961 tggggcgcgg aggcggtgct gctggggcac ggccgtccag cctcggcggc catatttttg
    10021 aggggctgtt catctcgttc acacgctctg tccgccatgt ttgtgagtgg aagcgccatt
    10081 accccttcaa gcgactgaag gctgcagggc ctctggtggc ccgcatgggg agaccagacc
    10141 cgccaggccc gcctttccgc actcagtccg ggcttacttt attttgtgag acagggtctc
    10201 gcctagag
//
