LOCUS       sequence-316815-        9412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral multicistronic vector for mammalian 
            expression of mCherry, H2B-mTagBFP2, and one gene of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9412)
  TITLE     pUltra series
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUltra-hot-H2B-mTagBFP2
FEATURES             Location/Qualifiers
     source          1..9412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1245..1254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1251..1958
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1396..1414)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1664..1683
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1968..2024
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2031..2408
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             3141..3194
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    3238..3826
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3291..3311)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3709..3720)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3917..4150
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4222..4343
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4259..4278)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4313..4332
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4383..4518
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4538..4557)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4539..4557)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4567..4584)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4567..4583)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4576..4598)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4725..5180
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4812..4831)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5022..5043
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5206..5310
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5311..5379,5380..6171)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5529..5548)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6342..6930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6831..6850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7084..7101
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7218..7239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7254..7259,7260..7277,7278..7284)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7292..7308
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7297..7319
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7316..7332
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7316..7332
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7351..7371
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7353..7371
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7399..7625
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7626..7806
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7853..7978
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8471..8704
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8889..8933
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9231..9348
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt ccgatccacc ggtcgccacc atggtgagca
     1261 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     1321 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     1381 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     1441 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     1501 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     1561 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     1621 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     1681 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1741 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1801 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1861 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1921 gccgccactc caccggcggc atggacgagc tgtacaaggg atctggagca acaaacttct
     1981 cactactcaa acaagcaggt gacgtggagg agaatcccgg gccttctaga atgccagagc
     2041 cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga
     2101 agaaaggcgg caagaagcgc aagcgcagcc gcaaggagag ctattccatc tatgtgtaca
     2161 aggttctgaa gcaggtccac cctgacaccg gcatttcgtc caaggccatg ggcatcatga
     2221 actcgtttgt gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt
     2281 acaacaagcg ctcgaccatc acctccaggg agatccagac ggccgtgcgc ctgctgctgc
     2341 ctggggagtt ggccaagcac gccgtgtccg agggtactaa ggccatcacc aagtacacca
     2401 gcgctaagga ccctccagtc gccaccatga gcgagttgat caaggaaaac atgcatatga
     2461 agctctacat ggaaggcacc gtggacaacc accatttcaa gtgtacctcc gagggagagg
     2521 gaaagcctta cgagggaact cagaccatgc gcatcaaggt cgtggaaggc ggtccactgc
     2581 ccttcgcttt cgacattctc gccacctcat tcctctacgg ctccaagact ttcatcaatc
     2641 atacccaggg tattcccgat ttcttcaagc aatcgttccc ggaagggttt acctgggaac
     2701 gcgtgaccac ttacgaggac ggaggggtcc tgaccgcaac tcaggacact agcctgcagg
     2761 acggttgcct gatctataac gtgaagattc ggggagtgaa cttcacctcc aacggacctg
     2821 tcatgcaaaa gaaaaccctg ggctgggaag cctttaccga gactctgtat ccggccgacg
     2881 gcggacttga ggggagaaat gatatggcgc tgaaactggt cggcggctcc cacctgatcg
     2941 ccaacgccaa gacgacctac cggtcgaaga agcccgccaa gaacctgaag atgccgggag
     3001 tgtactacgt ggactacagg ctggagcgga tcaaggaagc caacaacgaa acatacgtgg
     3061 aacagcacga agtggccgtg gcgcgctact gcgatctccc tagcaaactg gggcacaaac
     3121 tcaatggatc cggctccggc gagggcaggg gaagtctact aacatgcggg gacgtggagg
     3181 aaaatcccgg cccagctagc gtgtgatcag aattcgttcc ggagtcgtcg actcgacaat
     3241 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3301 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3361 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3421 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3481 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3541 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3601 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     3661 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3721 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcatcttcgc
     3781 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattcg
     3841 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     3901 aaagaaaagg ggggactgga agggctacgt aactcccaac gaagacaaga tctgcttttt
     3961 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     4021 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     4081 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4141 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     4201 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     4261 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4321 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     4381 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4441 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     4501 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     4561 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     4621 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     4681 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     4741 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4801 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4861 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4921 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4981 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5041 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5101 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5161 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5221 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5281 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5341 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5401 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5461 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5521 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5581 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5641 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5701 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5761 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5821 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5881 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5941 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6001 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6061 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6121 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6181 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6241 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6301 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6361 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6421 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6481 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6541 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6601 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6661 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6721 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6781 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6841 tggtatcttt atagtcctgt cgggtttcgc cacctctcac ttgagcgtcg atttttgtga
     6901 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6961 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7021 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7081 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7141 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7201 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7261 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7321 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     7381 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     7441 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     7501 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     7561 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     7621 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7681 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7741 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7801 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     7861 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     7921 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     7981 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     8041 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     8101 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     8161 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     8221 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     8281 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     8341 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     8401 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     8461 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     8521 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     8581 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     8641 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     8701 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     8761 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     8821 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     8881 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     8941 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     9001 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     9061 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     9121 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     9181 cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga
     9241 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     9301 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     9361 agggacagca gagatccagt ttggttaatt aacccgtgtc ggctccagat ct
//
