LOCUS       sequence-316819-       13014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dosage control and tracing of reprogramming episomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13014)
  AUTHORS   Schmitt CE, Morales BM, Schmitz EMH, Hawkins JS, Lizama CO, Zape JP,
            Hsiao EC, Zovein AC
  TITLE     Fluorescent tagged episomals for stoichiometric induced pluripotent 
            stem cell reprogramming.
  JOURNAL   Stem Cell Res Ther. 2017 Jun 5;8(1):132. doi: 
            10.1186/s13287-017-0581-7.
  PUBMED    28583172
REFERENCE   2  (bases 1 to 13014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther."; date: "2017-06-5"; volume: "8(1):132. doi"; pages: " 
            10.1186/s13287-017-0581-7"
COMMENT     Sequence Label: PCXLE-hUL + mTAGBFP2
FEATURES             Location/Qualifiers
     source          1..13014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          618..1634
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1558..1580
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1642..1661
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2815..2880
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     misc_feature    3585..4173
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3638..3658)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4056..4067)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4264..4283)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4329..4384
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4383..4402)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4745..4761)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4745..4761)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4758..4780)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4769..4785
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4793..4799,4800..4817,4818..4823))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4838..4859
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    4948..4981
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(5293..5994)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             complement(6004..6021)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6122..6146)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6143..6346)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6172..6192)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6347..6650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    8539..8572
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      8759..10548
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(11089..11106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11260..11848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11340..11359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12019..12810,12811..12879))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12642..12661
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12880..12984)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
      541 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
      601 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg
      661 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
      721 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
      781 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
      841 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc
      901 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg
      961 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag
     1021 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc
     1081 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
     1141 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
     1201 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg
     1261 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     1321 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     1381 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     1441 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     1501 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     1561 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     1621 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     1681 aagaattcgc ccttcaccat ggactacgac tcgtaccagc actatttcta cgactatgac
     1741 tgcggggagg atttctaccg ctccacggcg cccagcgagg acatctggaa gaaattcgag
     1801 ctggtgccat cgccccccac gtcgccgccc tggggcttgg gtcccggcgc aggggacccg
     1861 gcccccggga ttggtccccc ggagccgtgg cccggagggt gcaccggaga cgaagcggaa
     1921 tcccggggcc actcgaaagg ctggggcagg aactacgcct ccatcatacg ccgtgactgc
     1981 atgtggagcg gcttctcggc ccgggaacgg ctggagagag ctgtgagcga ccggctcgct
     2041 cctggcgcgc cccgggggaa cccgcccaag gcgtccgccg ccccggactg cactcccagc
     2101 ctcgaagccg gcaacccggc gcccgccgcc ccctgtccgc tgggcgaacc caagacccag
     2161 gcctgctccg ggtccgagag cccaagcgac tcggagaatg aagaaattga tgttgtgaca
     2221 gtagagaaga ggcagtctct gggtattcgg aagccggtca ccatcacggt gcgagcagac
     2281 cccctggatc cctgcatgaa gcatttccac atctccatcc atcagcaaca gcacaactat
     2341 gctgcccgtt ttcctccaga aagctgctcc caagaagagg cttcagagag gggtccccaa
     2401 gaagaggttc tggagagaga tgctgcaggg gaaaaggaag atgaggagga tgaagagatt
     2461 gtgagtcccc cacctgtaga aagtgaggct gcccagtcct gccaccccaa acctgtcagt
     2521 tctgatactg aggatgtgac caagaggaag aatcacaact tcctggagcg caagaggcgg
     2581 aatgacctgc gttcgcgatt cttggcgctg agggaccagg tgcccaccct ggccagctgc
     2641 tccaaggccc ccaaagtagt gatcctaagc aaggccttgg aatacttgca agccctggtg
     2701 ggggctgaga agaggatggc tacagagaaa agacagctcc gatgccggca gcagcagttg
     2761 cagaaaagaa ttgcatacct cactggctac ggagatctca aaattgtcgc tcctgtcaaa
     2821 caaactctta actttgattt actcaaactg gctggggatg tagaaagcaa tccaggtcca
     2881 ctcatgaaca attcgccctt caccatgggc tccgtgtcca accagcagtt tgcaggtggc
     2941 tgcgccaagg cggcagaaga ggcgcccgag gaggcgccgg aggacgcggc ccgggcggcg
     3001 gacgagcctc agctgctgca cggtgcgggc atctgtaagt ggttcaacgt gcgcatgggg
     3061 ttcggcttcc tgtccatgac cgcccgcgcc ggggtcgcgc tcgacccccc agtggatgtc
     3121 tttgtgcacc agagtaagct gcacatggaa gggttccgga gcttgaagga gggtgaggca
     3181 gtggagttca cctttaagaa gtcagccaag ggtctggaat ccatccgtgt caccggacct
     3241 ggtggagtat tctgtattgg gagtgagagg cggccaaaag gaaagagcat gcagaagcgc
     3301 agatcaaaag gagacaggtg ctacaactgt ggaggtctag atcatcatgc caaggaatgc
     3361 aagctgccac cccagcccaa gaagtgccac ttctgccaga gcatcagcca tatggtagcc
     3421 tcatgtccgc tgaaggccca gcagggccct agtgcacagg gaaagccaac ctactttcga
     3481 gaggaagaag aagaaatcca cagccctacc ctgctcccgg aggcacagaa ttgaaagggc
     3541 gaattgcctg caggaattca agcttcgggg actagtcata tgataatcaa cctctggatt
     3601 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3661 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3721 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3781 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3841 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3901 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3961 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     4021 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4081 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4141 cgagtcggat ctccctttgg gccgcctccc cgcatcggta caattcactc ctcaggtgca
     4201 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     4261 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     4321 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     4381 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     4441 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     4501 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     4561 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     4621 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     4681 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
     4741 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4801 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4861 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatctcaa
     4921 ttccgatcat attcaataac ccttaatata acttcgtata atgtatgcta tacgaagtta
     4981 ttaggtctga agaggagttt acgtccagcc aagcttagga tcaattctca tgtttgacag
     5041 cttatcatcg atggccggcc gccatagagc ccaccgcatc cccagcatgc ctgctattgt
     5101 cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc
     5161 tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac
     5221 cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag
     5281 gcacaaccgg tcttaattaa gcttgtgccc cagtttgcta gggaggtcgc agtatctggc
     5341 cactgccacc tcgtgctgct cgacgtaggt ctcgttgttg gcctccttga ttctttccag
     5401 tctgtagtcc acatagtaga cgccaggcat cttgaggttc ttagcgggtt tcttggatct
     5461 atatgtggtc ttggcgtttg cgatcagatg gctcccgccc acgagcttca gggccatgtc
     5521 gtttctgcct tccaggccgc cgtcagcggg gtacagcgtc tcggtgaagg cctcccagcc
     5581 gagtgttttc ttctgcatca cagggccgtt ggatgtgaag ttcacccctc tgatcttgac
     5641 gttgtagatg aggcagccgt cctggaggct ggtgtcctgg gtagcggtca gcacgccccc
     5701 gtcttcgtat gtggtgactc tctcccatgt gaagccctca gggaaggact gcttgaagaa
     5761 gtcggggatg ccctgggtgt ggttgatgaa ggtcttgctg ccgtagagga agctagtagc
     5821 caggatgtcg aaggcgaagg ggagagggcc gccctcgacc accttgattc tcatggtctg
     5881 ggtgccctcg tagggcttgc cttcgccctc ggatgtgcac ttgaagtgat ggttgtccac
     5941 ggtgccctcc atgtacagct tcatgtgcat gttctcctta atcagctcgc tcatagatct
     6001 gccatggtga tggtgatggt ggctccccat ctcgagatct gagtccggta gcgctagcga
     6061 tcctctagta gagtcggtgt cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg
     6121 cgatctgacg gttcactaaa cgagctctgc ttatatagac ctcccaccgt acacgcctac
     6181 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
     6241 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     6301 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
     6361 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
     6421 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     6481 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     6541 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     6601 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactccc
     6661 aagcttgcga tcgcatcgat aagctgatcc tcacaggccg cacccagctt ttcttccgtt
     6721 gccccagtag catctctgtc tggtgacctt gaagaggaag aggaggggtc ccgagaatcc
     6781 ccatccctac cgtccagcaa aaagggggac gaggaatttg aggcctggct tgaggctcag
     6841 gacgcaaatc ttgaggatgt tcagcgggag ttttccgggc tgcgagtaat tggtgatgag
     6901 gacgaggatg gttcggagga tggggaattt tcagacctgg atctgtctga cagcgaccat
     6961 gaaggggatg agggtggggg ggctgttgga gggggcagga gtctgcactc cctgtattca
     7021 ctgagcgtcg tctaataaag atgtctattg atctctttta gtgtgaatca tgtctgacga
     7081 ggggccaggt acaggacctg gaaatggcct aggagagaag ggagacacat ctggaccaga
     7141 aggctccggc ggcagtggac ctcaaagaag agggggtgat aaccatggac gaggacgggg
     7201 aagaggacga ggacgaggag gcggaagacc aggagccccg ggcggctcag gatcagggcc
     7261 aagacataga gatggtgtcc ggagacccca aaaacgtcca agttgcattg gctgcaaagg
     7321 gacccacggt ggaacaggag caggagcagg agcgggaggg gcaggagcag gaggggcagg
     7381 agcaggagga ggggcaggag caggaggggc aggagcagga ggtggaggcc ggggtcgagg
     7441 aggcagtgga ggccggggtc gaggaggtag tggaggccgg ggtcgaggag gtagtggagg
     7501 ccgccggggt agaggacgtg aaagagccag ggggggaagt cgtgaaagag ccagggggag
     7561 aggtcgtgga cgtggagaaa agaggcccag gagtcccagt agtcagtcat catcatccgg
     7621 gtctccaccg cgcaggcccc ctccaggtag aaggccattt ttccaccctg taggggaagc
     7681 cgattatttt gaataccacc aagaaggtgg cccagatggt gagcctgacg tgcccccggg
     7741 agcgatagag cagggccccg cagatgaccc aggagaaggc ccaagcactg gaccccgggg
     7801 tcagggtgat ggaggcaggc gcaaaaaagg agggtggttt ggaaagcatc gtggtcaagg
     7861 aggttccaac ccgaaatttg agaacattgc agaaggttta agagctctcc tggctaggag
     7921 tcacgtagaa aggactaccg acgaaggaac ttgggtcgcc ggtgtgttcg tatatggagg
     7981 tagtaagacc tccctttaca acctaaggcg aggaactgcc cttgctattc cacaatgtcg
     8041 tcttacacca ttgagtcgtc tcccctttgg aatggcccct ggacccggcc cacaacctgg
     8101 cccgctaagg gagtccattg tctgttattt catggtcttt ttacaaactc atatatttgc
     8161 tgaggttttg aaggatgcga ttaaggacct tgttatgaca aagcccgctc ctacctgcaa
     8221 tatcagggtg actgtgtgca gctttgacga tggagtagat ttgcctccct ggtttccacc
     8281 tatggtggaa ggggctgccg cggagggtga tgacggagat gacggagatg aaggaggtga
     8341 tggagatgag ggtgaggaag ggcaggagtg atgtaacttg ttaggagacg ccctcaatcg
     8401 tattaaaagc cgtgtattcc cccgcactaa agaataaatc cccagtagac atcatgcgtg
     8461 ctgttggtgt atttctggcc atctgtcttg tcaccatttt cgtcctccca acatggggca
     8521 attgccggaa cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtccc
     8581 tcgaagaggt tcactagcgg atctcaattg ggcataccca tgttgtcacg tcactcagct
     8641 ccgcgctcaa caccttctcg cgttggaaaa cattagcgac atttacctgg tgagcaatca
     8701 gacatgcgac ggctttagcc tggcctcctt aaattcacct aagaatggga gcaaccagca
     8761 ggaaaaggac aagcagcgaa aattcacgcc cccttgggag gtggcggcat atgcaaagga
     8821 tagcactccc actctactac tgggtatcat atgctgactg tatatgcatg aggatagcat
     8881 atgctacccg gatacagatt aggatagcat atactaccca gatatagatt aggatagcat
     8941 atgctaccca gatatagatt aggatagcct atgctaccca gatataaatt aggatagcat
     9001 atactaccca gatatagatt aggatagcat atgctaccca gatatagatt aggatagcct
     9061 atgctaccca gatatagatt aggatagcat atgctaccca gatatagatt aggatagcat
     9121 atgctatcca gatatttggg tagtatatgc tacccagata taaattagga tagcatatac
     9181 taccctaatc tctattagga tagcatatgc tacccggata cagattagga tagcatatac
     9241 tacccagata tagattagga tagcatatgc tacccagata tagattagga tagcctatgc
     9301 tacccagata taaattagga tagcatatac tacccagata tagattagga tagcatatgc
     9361 tacccagata tagattagga tagcctatgc tacccagata tagattagga tagcatatgc
     9421 tatccagata tttgggtagt atatgctacc catggcaaca ttagcccacc gtgctctcag
     9481 cgacctcgtg aatatgagga ccaacaaccc tgtgcttggc gctcaggcgc aagtgtgtgt
     9541 aatttgtcct ccagatcgca gcaatcgcgc ccctatcttg gcccgcccac ctacttatgc
     9601 aggtattccc cggggtgcca ttagtggttt tgtgggcaag tggtttgacc gcagtggtta
     9661 gcggggttac aatcagccaa gttattacac ccttatttta cagtccaaaa ccgcagggcg
     9721 gcgtgtgggg gctgacgcgt gcccccactc cacaatttca aaaaaaagag tggccacttg
     9781 tctttgttta tgggccccat tggcgtggag ccccgtttaa ttttcggggg tgttagagac
     9841 aaccagtgga gtccgctgct gtcggcgtcc actctctttc cccttgttac aaatagagtg
     9901 taacaacatg gttcacctgt cttggtccct gcctgggaca catcttaata accccagtat
     9961 catattgcac taggattatg tgttgcccat agccataaat tcgtgtgaga tggacatcca
    10021 gtctttacgg cttgtcccca ccccatggat ttctattgtt aaagatattc agaatgtttc
    10081 attcctacac tagtatttat tgcccaaggg gtttgtgagg gttatattgg tgtcatagca
    10141 caatgccacc actgaacccc ccgtccaaat tttattctgg gggcgtcacc tgaaaccttg
    10201 ttttcgagca cctcacatac accttactgt tcacaactca gcagttattc tattagctaa
    10261 acgaaggaga atgaagaagc aggcgaagat tcaggagagt tcactgcccg ctccttgatc
    10321 ttcagccact gcccttgtga ctaaaatggt tcactaccct cgtggaatcc tgaccccatg
    10381 taaataaaac cgtgacagct catggggtgg gagatatcgc tgttccttag gaccctttta
    10441 ctaaccctaa ttcgatagca tatgcttccc gttgggtaac atatgctatt gaattagggt
    10501 tagtctggat agtatatact actacccggg aagcatatgc tacccgttta gggttaacaa
    10561 gggggcctta taaacactat tgctaatgcc ctcttgaggg tccgcttatc ggtagctaca
    10621 caggcccctc tgattgacgt tggtgtagcc tcccgtagtc ttcctgggcc cctgggaggt
    10681 acatgtcccc cagcattggt gtaagagctt cagccaagag ttacacataa aggcaatgtt
    10741 gtgttgcagt ccacagactg caaagtctgc tccaggatga aagccactca gtgttggcaa
    10801 atgtgcacat ccatttataa ggatgtcaac tacagtcaga gaaccccttt gtgtttggtc
    10861 cccccccgtg tcacatgtgg aacagggccc agttggcaag ttgtaccaac caactgaagg
    10921 gattacatgc actgccccgc gaagaagggg cagagatgtc gtagtcaggt ttagttcgtc
    10981 cggggcgggg atcgatcctc tagagtcgac gctagcggat ccgctgcatt aatgaatcgg
    11041 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    11101 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    11161 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    11221 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    11281 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    11341 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    11401 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    11461 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    11521 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    11581 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    11641 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    11701 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    11761 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    11821 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    11881 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    11941 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    12001 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    12061 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    12121 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    12181 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    12241 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    12301 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    12361 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    12421 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    12481 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    12541 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    12601 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    12661 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    12721 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    12781 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    12841 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    12901 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    12961 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtc
//
