LOCUS       sequence-316830-        3101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Direct-expressing FlpO AAV Virus. Alias: AiP978 - 
            pAAV-AiE2004m-minBG-FlpO-WPRE-HGHpA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Graybuck LT, Daigle TL, Sedeno-Cortes AE, Walker M, Kalmbach B, Lenz
            GH, Morin E, Nguyen TN, Garren E, Bendrick JL, Kim TK, Zhou T, 
            Mortrud M, Yao S, Siverts LA, Larsen R, Gore BB, Szelenyi ER, Trader
            C, Balaram P, van Velthoven CTJ, Chiang M, Mich JK, Dee N, Goldy J, 
            Cetin AH, Smith K, Way SW, Esposito L, Yao Z, Gradinaru V, Sunkin 
            SM, Lein E, Levi BP, Ting JT, Zeng H, Tasic B
  TITLE     Enhancer viruses for combinatorial cell-subclass-specific labeling.
  JOURNAL   Neuron. 2021 Mar 23. pii: S0896-6273(21)00159-8. doi: 
            10.1016/j.neuron.2021.03.011.
  PUBMED    33789083
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2021 Mar 23. pii: S0896-6273(21)00159-8. doi: 
            10.1016/j.neuron.2021.03.011."
COMMENT     Sequence Label: AiP978-pAAV-mscRE4-minBGpromoter-FlpO-WPRE-hGHpA
FEATURES             Location/Qualifiers
     source          1..3101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      637..646
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(643..648,649..669,670..1941)
                     /codon_start=1
                     /product="nuclear-targeted site-specific recombinase"
                     /label=FLPo
                     /note="FLPo is a mouse codon-optimized fusion between the 
                     SV40 nuclear localization signal and a thermostable version
                     of the Saccharomyces cerevisiae site-specific recombinase 
                     FLP.  Recombination occurs at FRT sequences."
                     /translation="MAPKKKRKVMSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAA
                     ELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKK
                     LIPAWEFTIIPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESI
                     WEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGV
                     IIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEY
                     QLLKDNLVRSYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWS
                     DKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQL
                     KGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
     misc_feature    1966..2554
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2019..2039)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2437..2448)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2586..3062
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2785..2804)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 gcggccgcac gcgtataagc cttgggggca atcaaactat tacattgagt ccttggattt
       61 gctacaaatt acattttaaa tgcaatcatt ttataaaagc ttcaacactc acacttggaa
      121 gcgttaccct gttgaatatc actgactcac taacttgcat tgccatgcta acttgctttc
      181 agagagatct cagaacacat catcttctgc tatttcaata catgcacatt aatttcctat
      241 caacgtgtgc tgatcaggaa ctctgtaatc tggcaccggt gtttattttt attcctgtct
      301 attcctgttg gctcacgaaa agattgtttg agcaagtgtt ttatggtgag ttgtatcata
      361 tgtacattga tttaatctgc ccacattcag ttctacaagc ggagccaaaa aaatagagac
      421 aagcataatt ttcattcaac atgagcccct caatgcaagc caagtacctc atctggtgct
      481 cagctaaagc aacagcaatc tgttccaccc tggagacaca actggccaca gaaaacttag
      541 tgaaaagagg caatgctatg cacaggacaa atgagctcgg gctgggcata aaagtcaggg
      601 cagagccatc tattgcttac atttgcttct ggcgtggcca ccatggctcc taagaagaag
      661 aggaaggtga tgagccagtt cgacatcctg tgcaagaccc cccccaaggt gctggtgcgg
      721 cagttcgtgg agagattcga gaggcccagc ggcgagaaga tcgccagctg tgccgccgag
      781 ctgacctacc tgtgctggat gatcacccac aacggcaccg ccatcaagag ggccaccttc
      841 atgagctaca acaccatcat cagcaacagc ctgagcttcg acatcgtgaa caagagcctg
      901 cagttcaagt acaagaccca gaaggccacc atcctggagg ccagcctgaa gaagctgatc
      961 cccgcctggg agttcaccat catcccttac aacggccaga agcaccagag cgacatcacc
     1021 gacatcgtgt ccagcctgca gctgcagttc gagagcagcg aggaggccga caagggcaac
     1081 agccacagca agaagatgct gaaggccctg ctgtccgagg gcgagagcat ctgggagatc
     1141 accgagaaga tcctgaacag cttcgagtac accagcaggt tcaccaagac caagaccctg
     1201 taccagttcc tgttcctggc cacattcatc aactgcggca ggttcagcga catcaagaac
     1261 gtggacccca agagcttcaa gctggtgcag aacaagtacc tgggcgtgat cattcagtgc
     1321 ctggtgaccg agaccaagac aagcgtgtcc aggcacatct actttttcag cgccagaggc
     1381 aggatcgacc ccctggtgta cctggacgag ttcctgagga acagcgagcc cgtgctgaag
     1441 agagtgaaca ggaccggcaa cagcagcagc aacaagcagg agtaccagct gctgaaggac
     1501 aacctggtgc gcagctacaa caaggccctg aagaagaacg ccccctaccc catcttcgct
     1561 atcaagaacg gccctaagag ccacatcggc aggcacctga tgaccagctt tctgagcatg
     1621 aagggcctga ccgagctgac aaacgtggtg ggcaactgga gcgacaagag ggcctccgcc
     1681 gtggccagga ccacctacac ccaccagatc accgccatcc ccgaccacta cttcgccctg
     1741 gtgtccaggt actacgccta cgaccccatc agcaaggaga tgatcgccct gaaggacgag
     1801 accaacccca tcgaggagtg gcagcacatc gagcagctga agggcagcgc cgagggcagc
     1861 atcagatacc ccgcctggaa cggcatcatc agccaggagg tgctggacta cctgagcagc
     1921 tacatcaaca ggcggatctg agaattcgat atcaagctta tcgataatca acctctggat
     1981 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     2041 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     2101 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     2161 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     2221 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     2281 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     2341 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgccta tgttgccacc
     2401 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     2461 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     2521 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgagcgct gctcgagaga
     2581 tctacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca
     2641 ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg
     2701 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga
     2761 agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat
     2821 cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg
     2881 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga
     2941 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc
     3001 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
     3061 ttctgatttt gtaggtaacc acgtgcggac cgagcggccg c
//
