LOCUS       sequence-316842-        2523 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Direct-expressing EGFP AAV Virus. Alias: AiP981 - 
            pAAV-AiE2004m-minBG-EGFP-WPRE-HGHpA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Graybuck LT, Daigle TL, Sedeno-Cortes AE, Walker M, Kalmbach B, Lenz
            GH, Morin E, Nguyen TN, Garren E, Bendrick JL, Kim TK, Zhou T, 
            Mortrud M, Yao S, Siverts LA, Larsen R, Gore BB, Szelenyi ER, Trader
            C, Balaram P, van Velthoven CTJ, Chiang M, Mich JK, Dee N, Goldy J, 
            Cetin AH, Smith K, Way SW, Esposito L, Yao Z, Gradinaru V, Sunkin 
            SM, Lein E, Levi BP, Ting JT, Zeng H, Tasic B
  TITLE     Enhancer viruses for combinatorial cell-subclass-specific labeling.
  JOURNAL   Neuron. 2021 Mar 23. pii: S0896-6273(21)00159-8. doi: 
            10.1016/j.neuron.2021.03.011.
  PUBMED    33789083
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2021 Mar 23. pii: S0896-6273(21)00159-8. doi: 
            10.1016/j.neuron.2021.03.011."
COMMENT     Sequence Label: AiP981-pAAV-mscRE4-minBGpromoter-EGFP-WPRE-hGHpA
FEATURES             Location/Qualifiers
     source          1..2523
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      638..647
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(644..646,647..649,650..1363)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(644..646,647..649,650..1360)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(689..710)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(950..969)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1297..1318
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1388..1976
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1441..1461)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1859..1870)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2008..2484
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2207..2226)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 gcggccgcac gcgtataagc cttgggggca atcaaactat tacattgagt ccttggattt
       61 gctacaaatt acattttaaa tgcaatcatt ttataaaagc ttcaacactc acacttggaa
      121 gcgttaccct gttgaatatc actgactcac taacttgcat tgccatgcta acttgctttc
      181 agagagatct cagaacacat catcttctgc tatttcaata catgcacatt aatttcctat
      241 caacgtgtgc tgatcaggaa ctctgtaatc tggcaccggt gtttattttt attcctgtct
      301 attcctgttg gctcacgaaa agattgtttg agcaagtgtt ttatggtgag ttgtatcata
      361 tgtacattga tttaatctgc ccacattcag ttctacaagc ggagccaaaa aaatagagac
      421 aagcataatt ttcattcaac atgagcccct caatgcaagc caagtacctc atctggtgct
      481 cagctaaagc aacagcaatc tgttccaccc tggagacaca actggccaca gaaaacttag
      541 tgaaaagagg caatgctatg cacaggacaa atgagctcgg gctgggcata aaagtcaggg
      601 cagagccatc tattgcttac atttgcttct gggatccgcc accatggtga gcaagggcga
      661 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      721 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      781 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
      841 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
      901 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      961 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1021 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1081 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1141 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1201 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1261 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1321 cgccgccggg atcactctcg gcatggacga gctgtacaag taagaattcg atatcaagct
     1381 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1441 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     1501 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     1561 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     1621 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     1681 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     1741 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     1801 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     1861 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     1921 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     1981 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     2041 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     2101 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     2161 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     2221 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     2281 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     2341 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     2401 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     2461 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc
     2521 cgc
//
