LOCUS       sequence-316843-        2564 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Direct-expressing EGFP AAV Virus. Alias: AiP995 - 
            pAAV-AiE2010m-minBG-EGFP-WPRE-HGHpA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Graybuck LT, Daigle TL, Sedeno-Cortes AE, Walker M, Kalmbach B, Lenz
            GH, Morin E, Nguyen TN, Garren E, Bendrick JL, Kim TK, Zhou T, 
            Mortrud M, Yao S, Siverts LA, Larsen R, Gore BB, Szelenyi ER, Trader
            C, Balaram P, van Velthoven CTJ, Chiang M, Mich JK, Dee N, Goldy J, 
            Cetin AH, Smith K, Way SW, Esposito L, Yao Z, Gradinaru V, Sunkin 
            SM, Lein E, Levi BP, Ting JT, Zeng H, Tasic B
  TITLE     Enhancer viruses for combinatorial cell-subclass-specific labeling.
  JOURNAL   Neuron. 2021 Mar 23. pii: S0896-6273(21)00159-8. doi: 
            10.1016/j.neuron.2021.03.011.
  PUBMED    33789083
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2021 Mar 23. pii: S0896-6273(21)00159-8. doi: 
            10.1016/j.neuron.2021.03.011."
COMMENT     Sequence Label: AiP995-pAAV-mscRE10-minBGpromoter-EGFP-WPRE-hGHpA
FEATURES             Location/Qualifiers
     source          1..2564
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      679..688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(685..687,688..690,691..1404)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(685..687,688..690,691..1401)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(730..751)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(991..1010)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1338..1359
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1429..2017
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1482..1502)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1900..1911)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2049..2525
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2248..2267)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 gcggccgcac gcgtatgtgt cttttactct gatcctcctg tttttacctt ccaagtgctg
       61 gaatcacaga catataccac tgtgcatagc atcattacaa tgttatagtt tttcacacta
      121 tgccttgact ttttggaaag gcaaaccacc tcttggattt ctccttcctt ctctatctct
      181 ctctctctct cttcctccct ccgtccctcc atctcttcct ccttcccatt ttcttctctc
      241 cctatttgga cacaatataa aataatttag atgaggtgag ttaaattgtg aacaaagtat
      301 gtgcctatac atggttgtaa atcagcttat caaagtgtaa tattagaaga atttataaaa
      361 atgataaaat tcatactcaa agttctgtgt aaagcaataa tagctttatc tccttttagt
      421 tatcttgagt ctttctatga ctaacaactc cctcataggc atcttaaaga gcagtaagca
      481 taagtagatt ccaaatggga agggagaagt gtgaaccatc actttcatcc agacttgtag
      541 atatatctgc tgcattttca gaaaccagaa acagacagtg ttctttatct ccattgagtc
      601 tagtgtagca acagagctcg ggctgggcat aaaagtcagg gcagagccat ctattgctta
      661 catttgcttc tgggatccgc caccatggtg agcaagggcg aggagctgtt caccggggtg
      721 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      781 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      841 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      901 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      961 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1021 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1081 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1141 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     1201 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1261 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     1321 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1381 ggcatggacg agctgtacaa gtaagaattc gatatcaagc ttatcgataa tcaacctctg
     1441 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     1501 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     1561 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     1621 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     1681 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     1741 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     1801 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc
     1861 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     1921 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     1981 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgagc gctgctcgag
     2041 agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg
     2101 ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact
     2161 aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg
     2221 ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac
     2281 aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc
     2341 ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt
     2401 agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct
     2461 acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg
     2521 tccttctgat tttgtaggta accacgtgcg gaccgagcgg ccgc
//
