LOCUS       sequence-31686-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Hagting A, Jackman M, Simpson K, Pines J
  TITLE     Translocation of cyclin B1 to the nucleus at prophase requires a 
            phosphorylation-dependent nuclear import signal.
  JOURNAL   Curr Biol. 1999 Jul 1. 9(13):680-9.
  PUBMED    10395539
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            1999 Jul 1. 9(13):680-9."
COMMENT     Sequence Label: pCMX/cyclinB1 (17)
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             100..129
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgtgggcttt agcgagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa
       61 ttaatacgac tcactatagg gagacccaag cttatcatgg agcaaaaact tatatctgag
      121 gaggaccttt ccatggcgct ccgagccacc aggaactcga aaattaatgc tgaaaataag
      181 gcgaagatca acatggcagg cgcaaagcgc gttcctacgg cccctgctgc aacctccaag
      241 cccggactga ggccaagaac agctcttggg gacattggta acaaagtcag tgaacaactg
      301 caggccaaaa tgcctatgaa gaaggaagca aaaccttcag ctactggaaa agtcattgat
      361 aaaaaactac caaaacctct tgaaaaggta cctatgctgg tgccagtgcc agtgtctgag
      421 ccagtgccag agccagaacc tgagccagaa cctgagcctg ttaaagaaga aaaactttcg
      481 cctgagccta ttttggttga tactgcctct ccaagcccaa tggaaacatc tggatgtgcc
      541 cctgcagaag aagacctgtg tcaggctttc tctgatgtaa ttcttgcagt aaatgatgtg
      601 gatgcagaag atggagctga tccaaacctt tgtagtgaat atgtgaaaga tatttatgct
      661 tatctgagac aacttgagga agagcaagca gtcagaccaa aatacctact gggtcgggaa
      721 gtcactggaa acatgagagc catcctaatt gactggctag tacaggttca aatgaaattc
      781 aggttgttgc aggagaccat gtacatgact gtctccatta ttgatcggtt catgcagaat
      841 aattgtgtgc ccaagaagat gctgcagctg gttggtgtca ctgccatgtt tttgca
//
