LOCUS       sequence-316884-        6697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6697)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-SSTR3-N-17
FEATURES             Location/Qualifiers
     source          1..6697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1960..2637
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             2686..3363
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     polyA_signal    3485..3606
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3522..3541)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3576..3595
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3613..4068)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3750..3771)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3962..3981
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4095..4199
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4201..4558
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4201..4392
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4224..4244)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4409..4544
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4593..5387
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4593..5387
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4647..4666)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5257..5276
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5575..5594)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5619..5666
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5995..6583
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6484..6503
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagac cgccatggcc actgttacct atccttcatc tgagcctacg
      661 accttggacc ctgggaacgc atcctcgacc tggcccctgg ataccaccct ggggaacaca
      721 tccgctggcg ctagcctgac aggcctggct gtcagtggca tcttgatctc tctggtgtac
      781 ctggtggtgt gcgtggtggg tttgctgggc aactcgctgg tgatctacgt ggtcctgcgg
      841 cacacgtcca gcccatcagt gaccagtgtc tatatcctca acctggctct ggctgatgag
      901 ctcttcatgc tagggctacc cttcctggct gctcagaacg ccctgtccta ctggcccttc
      961 ggatctctca tgtgccgtct ggtcatggcc gtggatggca tcaaccagtt caccagcatc
     1021 ttctgcctca ccgtcatgag tgtggaccgc tatctggctg tggtgcaccc cacacgctca
     1081 gcccgctggc gcacggcacc agtggctcgc acggtcagtg cagctgtctg ggtggcctcg
     1141 gctgtggtgg tgctgcctgt ggttgtgttc tcaggagtgc cccggggcat gagcacgtgc
     1201 cacatgcagt ggccagagcc agcggctgcc tggcgaacag ccttcatcat ctacacggcc
     1261 gcactgggct tctttgggcc cctgctggtc atctgcttgt gctacttgct cattgtggta
     1321 aaggtgcggt cgaccacccg gcgggtgcgg gcgccctcgt gtcagtgggt acaggcaccc
     1381 gcatgccagc ggcgacgccg ctctgagcgc agggtcacac gcatggtggt ggccgtggtg
     1441 gcactcttcg tcctctgctg gatgcctttc tatctgctca acatcgtcaa tgtggtgtgc
     1501 ccgctgccgg aggagcccgc cttcttcggc ctctacttcc tggtggtggc gctgccctac
     1561 gccaacagct gcgcaaaccc catcctctac ggcttcctct cctaccgctt caagcagggc
     1621 tttcgcagga tcctgctaag accatcacgt cgcattcgga gccaggagcc agggtcggga
     1681 cctccagaga agactgaaga ggaggaggat gaagaagaag aagagagaag ggaagaggag
     1741 gagcggagga tgcagagagg gcaggagatg aacgggaggc tcagtcagat cgcacaggct
     1801 ggcactagtg gacaacagcc acggccctgc acagggactg ctaaggagca gcagcttctg
     1861 ccccaggagg ccacagctgg ggacaaggcc agcacactga gccatctggg aattctgcag
     1921 tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tgagtgcgat taagccagac
     1981 atgaagatca acctccgtat ggaaggcaac gtaaacgggc accactttgt gatcgacgga
     2041 gatggtacag gcaagccttt tgagggaaaa cagagtatgg atcttgaagt caaagagggc
     2101 ggacctctgc cttttgcctt tgatatcctg accactgcat tccattacgg caacagggta
     2161 ttcgccgaat atccagacca catacaagac tattttaagc agtcgtttcc taaggggtat
     2221 tcgtgggaac gaagcttgac tttcgaagac gggggcattt gcattgccag aaacgacata
     2281 acaatggaag gggacacttt ctataataaa gttcgatttc acggtgtaaa ctttcccgcc
     2341 aatggtccag ttatgcagaa gaagacgctg aaatgggagc cctccactga gaaaatgtat
     2401 gtgcgtgatg gagtgctgac tggtgatatt cgcatggctt tgttgcttga aggaaatgcc
     2461 cattaccgat gtgacttcag aactacttac aaagctaagg agaagggtgt caagttacca
     2521 ggctaccact ttgtggacca ctgcattgag attttaagcc atgacaaaga ttacaacaag
     2581 gttaagctgt atgagcatgc tgttgctcat tctggattgc ctgacaatgc cagacgatcc
     2641 ggtagcggtc acggtactgg ttctactggt tctggttctt ctgagagtgc gattaagcca
     2701 gacatgaaga tcaacctccg tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac
     2761 ggagatggta caggcaagcc ttttgaggga aaacagagta tggatcttga agtcaaagag
     2821 ggcggacctc tgccttttgc ctttgatatc ctgaccactg cattccatta cggcaacagg
     2881 gtattcgccg aatatccaga ccacatacaa gactatttta agcagtcgtt tcctaagggg
     2941 tattcgtggg aacgaagctt gactttcgaa gacgggggca tttgcattgc cagaaacgac
     3001 ataacaatgg aaggggacac tttctataat aaagttcgat ttcacggtgt aaactttccc
     3061 gccaatggtc cagttatgca gaagaagacg ctgaaatggg agccctccac tgagaaaatg
     3121 tatgtgcgtg atggagtgct gactggtgat attcgcatgg ctttgttgct tgaaggaaat
     3181 gcccattacc gatgtgactt cagaactact tacaaagcta aggagaaggg tgtcaagtta
     3241 ccaggctacc actttgtgga ccactgcatt gagattttaa gccatgacaa agattacaac
     3301 aaggttaagc tgtatgagca tgctgttgct cattctggat tgcctgacaa tgccagacga
     3361 taagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     3421 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     3481 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     3541 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     3601 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     3661 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     3721 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     3781 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     3841 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     3901 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     3961 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     4021 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     4081 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4141 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
     4201 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4261 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4321 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4381 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4441 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4501 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     4561 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     4621 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     4681 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     4741 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     4801 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     4861 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     4921 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     4981 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     5041 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     5101 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     5161 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     5221 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     5281 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     5341 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     5401 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     5461 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     5521 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     5581 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     5641 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     5701 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     5761 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     5821 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     5881 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5941 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6001 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6061 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6121 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6181 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6241 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6301 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6361 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6421 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6481 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6541 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6601 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6661 ccctgattct gtggataacc gtattaccgc catgcat
//
