LOCUS       sequence-31689-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty backbone for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown 
            NA, Gileadi O
  TITLE     High-throughput production of human proteins for crystallization: 
            the SGC experience.
  JOURNAL   J Struct Biol. 2010 Oct;172(1):3-13. doi: 10.1016/j.jsb.2010.06.008.
            Epub 2010 Jun 10.
  PUBMED    20541610
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jsb.2010.06.008"; journalName: "J Struct Biol"; date: 
            "2010-10"; volume: "172"; issue: "1"; pages: "3-13"
COMMENT     Sequence Label: pNH-TrxT
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..389
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             396..416
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     promoter        432..877
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtatgagcga taaaattatt cacctgactg acgacagttt tgacacggat gtactcaaag
      121 cggacggggc gatcctcgtc gatttctggg cagagtggtg cggtccgtgc aaaatgatcg
      181 ccccgattct ggatgaaatc gctgacgaat atcagggcaa actgaccgtt gcaaaactga
      241 acatcgatca aaaccctggc actgcgccga aatatggcat ccgtggtatc ccgactctgc
      301 tgctgttcaa aaacggtgaa gtggcggcaa ccaaagtggg cgcactgtct aaaggtcagt
      361 tgaaagagtt cctcgacgct aacctggccg gtaccgagaa cttgtacttc caatccatgg
      421 agaccgacgt ccacatatac ctgccgttca ctattattta gtgaaatgag atattatgat
      481 attttctgaa ttgtgattaa aaaggcaact ttatgcccat gcaacagaaa ctataaaaaa
      541 tacagagaat gaaaagaaac agatagattt tttagttctt taggcccgta gtctgcaaat
      601 ccttttatga ttttctatca aacaaaagag gaaaatagac cagttgcaat ccaaacgaga
      661 gtctaataga atgaggtcga aaagtaaatc gcgcgggttt gttactgata aagcaggcaa
      721 gacctaaaat gtgtaaaggg caaagtgtat actttggcgt caccccttac atattttagg
      781 tcttttttta ttgtgcgtaa ctaacttgcc atcttcaaac aggagggctg gaagaagcag
      841 accgctaaca cagtacataa aaaaggagac atgaacgatg aacatcaaaa agtttgcaaa
      901 acaagca
//
