LOCUS       sequence-316899-        1163 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to construct lentivirus to express membrane-targeted mCherry in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1163)
  TITLE     Expression plasmid for production of murine IFN gamma
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mem-mCherry-pTRIP
FEATURES             Location/Qualifiers
     source          1..1163
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        60..159
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     110..130
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     156..180
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             354..1061
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(499..517)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     767..786
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
       61 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      121 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
      181 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact ctagaggccg
      241 gccgatagtt taaacggccg gccatgctgt gctgtatgag aagaaccaaa caggttgaaa
      301 agaatgatga ggaccaaaag atcggctgat cgatgctagc gctaccggtc accatggtga
      361 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
      421 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      481 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
      541 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
      601 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
      661 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
      721 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
      781 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
      841 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
      901 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
      961 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     1021 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtccggactc agatctcgag
     1081 aacccgggaa ggatccaccg gatctagata actgatcata attctaccgg gtaggggagg
     1141 cgcttttccc aaggcagtct gga
//
