LOCUS       sequence-316900-        8447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Nallamsetty S, Austin BP, Penrose KJ, Waugh DS
  TITLE     Gateway vectors for the production of combinatorially-tagged 
            His6-MBP fusion proteins in the cytoplasm and periplasm of 
            Escherichia coli.
  JOURNAL   Protein Sci. 2005 Dec . 14(12):2964-71.
  PUBMED    16322578
REFERENCE   2  (bases 1 to 8447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2005 Dec . 14(12):2964-71."
COMMENT     Sequence Label: pDEST-HisMBP
FEATURES             Location/Qualifiers
     source          1..8447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(129..434)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(231..250)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(776..1435)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1350..1369
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1436..1538)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(1712..1836)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1849..1872)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     regulatory      1980..1989
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2938..2955)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3023..3045)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3034..3050
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3058..3064,3065..3080,3081..3086))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             complement(3329..4411)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4373..4392
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4412..4489)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4985..5176
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(5170..5192)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5278..5418
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5333..5352
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5433..5450)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5604..6192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5684..5703)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      6303..6816
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6390..6409)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6600..6621
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(6858..7649,7650..7718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7481..7500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7719..7810)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(7829..7856)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(7948..8034)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     8187..8204
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     8368..8390
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8382..8399
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8383..8399
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(8411..8447,1..88)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
       61 gtaaaacaca acatatccag tcactatggt cgacctgcag actggctgtg tataagggag
      121 cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg
      181 tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg
      241 gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggagact
      301 ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca
      361 ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa
      421 accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt tcatttcaat
      481 aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacgcag
      541 acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga catcatatat
      601 gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg cttcatagca
      661 tacctctttt tgacatactt cgggtataca tatcagtata tattcttata ccgcaaaaat
      721 cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccggatcca cgcgtttacg
      781 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
      841 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
      901 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
      961 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
     1021 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     1081 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     1141 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     1201 ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     1261 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     1321 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     1381 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     1441 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     1501 cattatggtg aaagttggaa cctcttgcgt gccgatcaac gtctcatttt cgccaaaagt
     1561 tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat
     1621 cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc aacttagcgg
     1681 ccgctaagtt ggcagcatca cccgcggccg ccatagtgac tggatatgtt gtgttttaca
     1741 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
     1801 ttacgtttct cgttcagctt ttttgtacaa acttgtgatc gaattagtct gcgcgtcttt
     1861 cagggcttca tcgacagtct gacgaccgct ggcggcgttg atcaccgcag tacgcacggc
     1921 ataccagaaa gcggacatct gcgggatgtt cggcatgatt tcacctttct gggcgttttc
     1981 catggtggcg gcaatacgtg gatctttcgc caactcttcc tcgtaagact tcagcgctac
     2041 ggcacccagc ggtttgtctt tattaaccgc ttccagacct tcatcagtca gcagatagtt
     2101 ttcgaggaac tcttttgcca gctctttgtt cggactggcg gcgttaatac ctgcgctcag
     2161 cacgccaacg aacggtttgg atggttgacc cttgaaggtc ggcagtaccg ttacaccata
     2221 attcactttg ctggtgtcga tgttggacca tgcccacggg ccgttgatgg tcatcgctgt
     2281 ttcgccttta ttaaaggcag cttctgcgat ggagtaatcg gtgtctgcat tcatgtgttt
     2341 gtttttaatc aggtcaacca ggaaggtcag acccgctttc gcgccagcgt tatccacgcc
     2401 cacgtcttta atgtcgtact tgccgttttc atacttgaac gcataacccc cgtcagcagc
     2461 aatcagcggc caggtgaagt acggttcttg caggttgaac atcagcgcgc tcttaccttt
     2521 cgctttcagt tctttatcca gcgccgggat ctcttcccag gtttttggcg ggttcggcag
     2581 cagatctttg ttataaatca gcgataacgc ttcaacagcg atcgggtaag caatcagctt
     2641 gccgttgtaa cgtacggcat cccaggtaaa cggatacagc ttgtcctgga acgctttgtc
     2701 cggggtgatt tcagccaaca ggccagattg agcgtagcca ccaaagcggt cgtgtgccca
     2761 gaagataatg tcagggccat cgccagttgc cgcaacctgt gggaatttct cttccagttt
     2821 atccggatgc tcaacggtga ctttaattcc ggtatctttc tcgaatttct taccgacttc
     2881 agcgagaccg ttatagcctt tatcgccgtt aatccagatt accagtttac cttcttcgtg
     2941 atggtgatgg tgatggattt tcataatcta tggtccttgt tggtgaagtg ctcgtgaaaa
     3001 cacctaaacg gactggctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacat
     3061 tatacgagcc gatgattaat tgtcaacagc tcatttcaga atatttgcca gaaccgttat
     3121 gatgtcggcg caaaaaacat tatccagaac gggagtgcgc cttgagcgac acgaattatg
     3181 cagtgattta cgacctgcac agccatacca cagcttccga tggctgcctg acgccagaag
     3241 cattggtgca ccgtgcagtc gatgataagc tgtcaaacat gagaattgtg cctaatgagt
     3301 gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3361 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3421 ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct
     3481 ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct
     3541 gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca
     3601 ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca
     3661 gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt
     3721 gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct
     3781 gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag
     3841 aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca
     3901 cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag
     3961 agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct
     4021 ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca
     4081 ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac
     4141 ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca
     4201 gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc
     4261 ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag
     4321 aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact
     4381 ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg
     4441 ggcgctatca tgccataccg cgaaaggttt tgcaccattc gatggtgtcg gaatttcggg
     4501 cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct
     4561 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg
     4621 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt
     4681 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat
     4741 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg
     4801 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt
     4861 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat
     4921 cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc
     4981 atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac
     5041 attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga
     5101 atcgctgcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga
     5161 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     5221 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     5281 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     5341 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     5401 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5461 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5521 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5581 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5641 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5701 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5761 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5821 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5881 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5941 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6001 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     6061 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6121 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6181 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6241 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6301 ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6361 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6421 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6481 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     6541 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6601 tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt
     6661 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     6721 taacgcgaat tttaacaaaa tattaacgtt tacaatttaa atatttgctt atacaatctt
     6781 cctgtttttg gggcttttct gattatcaac cggggtaaat caatctaaag tatatatgag
     6841 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6901 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6961 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7021 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7081 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7141 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7201 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7261 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7321 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7381 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7441 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     7501 agaactttaa aagtgctcat cattgggaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7561 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7621 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7681 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7741 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7801 aataaacaaa agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     7861 aatttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     7921 cgcaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     7981 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct
     8041 ggcagttccc tactctcgca tggggagacc ccacactacc atcggcgcta cggcgtttca
     8101 cttctgagtt cggcatgggg tcaggtggga ccaccgcgct actgccgcca ggcaaattct
     8161 gttttatcag accgcttctg cgttctgatt taatctgtat caggctgaaa atcttatctc
     8221 atccgccaaa acagccaagc tgccattcgc cattcaggct gcgcaactgt tgggaagggc
     8281 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     8341 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     8401 ccaagctatc accactttgt acaagaaagc tgaacgagaa acgtaaa
//
