LOCUS       sequence-316901-        9627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A:T-to-G:C base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Koblan LW, Doman JL, Wilson C, Levy JM, Tay T, Newby GA, Maianti JP,
            Raguram A, Liu DR
  TITLE     Improving cytidine and adenine base editors by expression 
            optimization and ancestral reconstruction.
  JOURNAL   Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.
  PUBMED    29813047
REFERENCE   2  (bases 1 to 9627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172."
COMMENT     Sequence Label: pCMV_ABEmax_P2A_GFP
FEATURES             Location/Qualifiers
     source          1..9627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             62..82
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             92..148
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(149..151,152..154,155..865)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(149..151,152..154,155..865)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(455..474)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     802..823
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             878..898
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             907..924
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(947..964)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    953..1177
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1248..1264)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1248..1264)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1261..1283)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1272..1288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1296..1302,1303..1320,1321..1326))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1341..1362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1479..1496)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1650..2238)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1730..1749)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2409..3200,3201..3269))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3032..3051
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3270..3374)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3453..3472)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3644..4023
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4024..4227
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4178..4198
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4269..4288
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4269..4287
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4337..4357
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5546..5569,5570..5572,5573..9627,1..19)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gtctcagctg ggaggtgact ctggcggctc aaaaagaacc gccgacggca gcgaattcga
       61 gcccaagaag aagaggaaag tcggaagcgg agctactaac ttcagcctgc tgaagcaggc
      121 tggagacgtg gaggagaacc ctggacctat ggtgagcaag ggcgaggagc tgttcaccgg
      181 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      241 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      301 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctatg gagtgcagtg
      361 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      421 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      481 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      541 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
      601 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
      661 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      721 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
      781 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      841 tctcggcatg gacgagctgt acaagtctgg tggttctccc aagaagaaga ggaaagtcta
      901 accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct
      961 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     1021 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     1081 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     1141 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     1201 tggggctcga taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     1261 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     1321 tgtaaagcct agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1381 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1441 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1501 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1561 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1621 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1681 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1741 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1801 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1861 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1921 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1981 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2041 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2101 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2161 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2221 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cactcagtgg
     2281 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2341 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2401 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2461 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2521 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2581 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2641 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2701 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2761 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2821 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2881 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2941 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3001 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3061 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3121 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3181 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3241 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3301 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3361 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     3421 cgatctcccg atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta
     3481 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     3541 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     3601 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     3661 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     3721 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     3781 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     3841 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     3901 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     3961 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     4021 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     4081 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     4141 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     4201 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagagatcc
     4261 gcggccgcta atacgactca ctatagggag agccgccacc atgaaacgga cagccgacgg
     4321 aagcgagttc gagtcaccaa agaagaagcg gaaagtctct gaagtcgagt ttagccacga
     4381 gtattggatg aggcacgcac tgaccctggc aaagcgagca tgggatgaaa gagaagtccc
     4441 cgtgggcgcc gtgctggtgc acaacaatag agtgatcgga gagggatgga acaggccaat
     4501 cggccgccac gaccctaccg cacacgcaga gatcatggca ctgaggcagg gaggcctggt
     4561 catgcagaat taccgcctga tcgatgccac cctgtatgtg acactggagc catgcgtgat
     4621 gtgcgcagga gcaatgatcc acagcaggat cggaagagtg gtgttcggag cacgggacgc
     4681 caagaccggc gcagcaggct ccctgatgga tgtgctgcac caccccggca tgaaccaccg
     4741 ggtggagatc acagagggaa tcctggcaga cgagtgcgcc gccctgctga gcgatttctt
     4801 tagaatgcgg agacaggaga tcaaggccca gaagaaggca cagagctcca ccgactctgg
     4861 aggatctagc ggaggatcct ctggaagcga gacaccaggc acaagcgagt ccgccacacc
     4921 agagagctcc ggcggctcct ccggaggatc ctctgaggtg gagttttccc acgagtactg
     4981 gatgagacat gccctgaccc tggccaagag ggcacgcgat gagagggagg tgcctgtggg
     5041 agccgtgctg gtgctgaaca atagagtgat cggcgagggc tggaacagag ccatcggcct
     5101 gcacgaccca acagcccatg ccgaaattat ggccctgaga cagggcggcc tggtcatgca
     5161 gaactacaga ctgattgacg ccaccctgta cgtgacattc gagccttgcg tgatgtgcgc
     5221 cggcgccatg atccactcta ggatcggccg cgtggtgttt ggcgtgagga acgcaaaaac
     5281 cggcgccgca ggctccctga tggacgtgct gcactacccc ggcatgaatc accgcgtcga
     5341 aattaccgag ggaatcctgg cagatgaatg tgccgccctg ctgtgctatt tctttcggat
     5401 gcctagacag gtgttcaatg ctcagaagaa ggcccagagc tccaccgact ccggaggatc
     5461 tagcggaggc tcctctggct ctgagacacc tggcacaagc gagagcgcaa cacctgaaag
     5521 cagcgggggc agcagcgggg ggtcagacaa gaagtacagc atcggcctgg ccatcggcac
     5581 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
     5641 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
     5701 cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
     5761 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
     5821 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
     5881 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
     5941 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
     6001 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
     6061 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
     6121 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
     6181 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
     6241 gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
     6301 gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga
     6361 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
     6421 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
     6481 caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac
     6541 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
     6601 ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta
     6661 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
     6721 gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca
     6781 gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct
     6841 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
     6901 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
     6961 caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga
     7021 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
     7081 gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg
     7141 aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt
     7201 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
     7261 gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
     7321 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
     7381 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
     7441 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
     7501 gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
     7561 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
     7621 catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
     7681 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
     7741 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg
     7801 gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
     7861 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
     7921 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
     7981 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
     8041 gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga
     8101 caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga
     8161 agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac
     8221 ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
     8281 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
     8341 gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt
     8401 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
     8461 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
     8521 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
     8581 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
     8641 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
     8701 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt
     8761 gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa
     8821 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa
     8881 gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
     8941 cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
     9001 gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag
     9061 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
     9121 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
     9181 gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
     9241 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
     9301 gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat
     9361 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc
     9421 ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt
     9481 taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg
     9541 gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac
     9601 cggcctgtac gagacacgga tcgacct
//
