LOCUS       sequence-316907-       10133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral vector expressing tTA-VP16 and EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10133)
  AUTHORS   Kafri T, van Praag H, Gage FH, Verma IM
  TITLE     Lentiviral vectors: regulated gene expression.
  JOURNAL   Mol Ther. 2000 Jun . 1(6):516-21.
  PUBMED    10933976
REFERENCE   2  (bases 1 to 10133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2000 Jun . 1(6):516-21."
COMMENT     Sequence Label: pCL-CTIG
FEATURES             Location/Qualifiers
     source          1..10133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(38..56,57..79,80..98,99..121,122..140,141..163,
                     164..182,183..205,206..224,225..247,248..266,267..289,
                     290..308)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        341..379
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      525..534
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(531..533,534..536,537..1247)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(531..533,534..536,537..1247)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(576..597)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(837..856)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1184..1205
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             1464..2097
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2123..2140)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2129..2353
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2399..2827
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2486..2505)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2696..2717
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2836..2856)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2841..3170
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3021..3156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3083..3102
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3218..3265
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3284..3658
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3788..3909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3825..3844)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3879..3898
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3958..3974)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3958..3974)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3971..3993)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3982..3998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4006..4012,4013..4030,4031..4036))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4051..4072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4189..4206)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4360..4948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4440..4459)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5119..5910,5911..5979))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5742..5761
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5980..6084)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6159..6178)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6350..6729
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6731..6929
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             6947..7127
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7174..7299
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7792..8025
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8210..8254
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        8578..8881
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8882..9085
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             join(9116..9736,9737..10123)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(9189..9208)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gatcccccgg gctgcaggaa ttcctcgagt ttaccactcc ctatcagtga tagagaaaag
       61 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
      121 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      181 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      241 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      301 gatagagaaa agtgaaagtc gagctcggta cccgggtcga ggtaggcgtg tacggtggga
      361 ggcctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
      421 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccgaattcga
      481 tcgttggttt aaaccaacaa ccggtacctc tagcgctacc ggtcgccacc atggtgagca
      541 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      601 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      661 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      721 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
      781 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      841 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      901 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      961 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1021 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1081 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1141 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1201 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat
     1261 cctactagta ggatctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     1321 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     1381 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     1441 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     1501 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     1561 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     1621 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     1681 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     1741 agcatttcat cacatggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     1801 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     1861 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     1921 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     1981 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     2041 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     2101 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     2161 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     2221 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     2281 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     2341 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     2401 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     2461 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2521 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2581 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     2641 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2701 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     2761 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2821 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     2881 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     2941 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     3001 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3061 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     3121 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     3181 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     3241 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     3301 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     3361 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     3421 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     3481 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     3541 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     3601 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     3661 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3841 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3901 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     3961 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4021 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4081 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4141 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4201 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4261 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4321 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4381 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4441 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4501 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4561 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4621 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4681 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4741 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4801 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4861 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4921 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4981 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5041 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5101 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5161 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5221 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5281 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5341 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5401 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5461 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5521 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5581 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5641 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5701 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5761 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5821 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5881 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5941 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6001 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6061 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     6121 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     6181 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     6241 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     6301 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     6361 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     6421 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     6481 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     6541 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     6601 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     6661 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     6721 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     6781 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     6841 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     6901 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     6961 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7021 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7081 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     7141 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     7201 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     7261 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     7321 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     7381 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     7441 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     7501 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     7561 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     7621 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     7681 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     7741 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     7801 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     7861 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     7921 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     7981 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     8041 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     8101 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     8161 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     8221 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     8281 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     8341 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     8401 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     8461 agagagagac agagacagat ccattcgatt agtgaacgga tcccccgggc tgcaggaatt
     8521 cgattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     8581 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     8641 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     8701 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     8761 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     8821 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     8881 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     8941 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     9001 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     9061 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agaccatggc
     9121 tagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat
     9181 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta
     9241 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag
     9301 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa
     9361 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg
     9421 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca
     9481 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt
     9541 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac
     9601 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc
     9661 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa
     9721 atgtgaaagt gggtccgcgt acagccgcgc gcgtacgaaa aacaattacg ggtctaccat
     9781 cgagggcctg ctcgatctcc cggacgacga cgcccccgaa gaggcggggc tggcggctcc
     9841 gcgcctgtcc tttctccccg cgggacacac gcgcagactg tcgacggccc ccccgaccga
     9901 tgtcagcctg ggggacgagc tccacttaga cggcgaggac gtggcgatgg cgcatgccga
     9961 cgcgctagac gatttcgatc tggacatgtt gggggacggg gattccccgg gtccgggatt
    10021 taccccccac gactccgccc cctacggcgc tctggatatg gccgacttcg agtttgagca
    10081 gatgtttacc gatgcccttg gaattgacga gtacggtggg tagggggcgc gag
//
