LOCUS       sequence-31691-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty backbone for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown 
            NA, Gileadi O
  TITLE     High-throughput production of human proteins for crystallization: 
            the SGC experience.
  JOURNAL   J Struct Biol. 2010 Oct;172(1):3-13. doi: 10.1016/j.jsb.2010.06.008.
            Epub 2010 Jun 10.
  PUBMED    20541610
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jsb.2010.06.008"; journalName: "J Struct Biol"; date: 
            "2010-10"; volume: "172"; issue: "1"; pages: "3-13"
COMMENT     Sequence Label: pNIC28-Bsa4
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             144..161
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             186..206
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     promoter        222..667
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacat atgcaccatc atcatcatca ttcttctggt gtagatctgg
      181 gtaccgagaa cctgtacttc caatccatgg agaccgacgt ccacatatac ctgccgttca
      241 ctattattta gtgaaatgag atattatgat attttctgaa ttgtgattaa aaaggcaact
      301 ttatgcccat gcaacagaaa ctataaaaaa tacagagaat gaaaagaaac agatagattt
      361 tttagttctt taggcccgta gtctgcaaat ccttttatga ttttctatca aacaaaagag
      421 gaaaatagac cagttgcaat ccaaacgaga gtctaataga atgaggtcga aaagtaaatc
      481 gcgcgggttt gttactgata aagcaggcaa gacctaaaat gtgtaaaggg caaagtgtat
      541 actttggcgt caccccttac atattttagg tcttttttta ttgtgcgtaa ctaacttgcc
      601 atcttcaaac aggagggctg gaagaagcag accgctaaca cagtacataa aaaaggagac
      661 atgaacgatg aacatcaaaa agtttgcaaa acaagcaaca gtattaacct ttactaccgc
      721 actgctggca ggaggcgcaa ctcaagcgtt tgcgaaagaa acgaaccaaa agccatataa
      781 ggaaacatac ggcatttccc atattacacg ccatgatatg ctgcaaatcc ctgaacagca
      841 aaaaaatgaa aaatataaag t
//
