LOCUS       sequence-316910-        7776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human beta-catenin with Flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Kolligs FT, Hu G, Dang CV, Fearon ER
  TITLE     Neoplastic transformation of RK3E by mutant beta-catenin requires 
            deregulation of Tcf/Lef transcription but not activation of c-myc 
            expression.
  JOURNAL   Mol Cell Biol. 1999 Aug . 19(8):5696-706.
  PUBMED    10409758
REFERENCE   2  (bases 1 to 7776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Aug . 19(8):5696-706."
COMMENT     Sequence Label: human beta-catenin pcDNA3
FEATURES             Location/Qualifiers
     source          1..7776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(57..75)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(58..75)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(95..112)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    101..325
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      371..799
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(458..477)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     668..689
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(808..828)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        813..1142
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      993..1128
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1055..1074
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1209..2003
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1263..1282)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1873..1892
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2177..2298
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2214..2233)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2268..2287
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2347..2363)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2347..2363)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2360..2382)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2371..2387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2395..2401,2402..2419,2420..2425))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2440..2461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2578..2595)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2749..3337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2829..2848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3508..4299,4300..4368))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4131..4150
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4369..4473)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4548..4567)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4739..5118
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5119..5322
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5273..5293
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5367..5386
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5367..5385
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5429..7771
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     CDS             5429..7771
                     /codon_start=1
                     /gene="Homo sapiens CTNNB1"
                     /product="human cadherin-associated protein that also plays
                     a role in the canonical Wnt signaling pathway"
                     /label=beta-catenin
                     /note="also known as catenin beta-1"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     CDS             join(7772..7776,1..19)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 caaggacgac gatgacaagt aagcggccgc tcgagcatgc atctagaggg ccctattcta
       61 tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
      121 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
      181 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
      241 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
      301 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
      361 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      421 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      481 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      541 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      601 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
      661 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
      721 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
      781 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
      841 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
      901 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
      961 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     1021 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     1081 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     1141 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     1201 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     1261 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     1321 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     1381 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     1441 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     1501 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     1561 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     1621 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     1681 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     1741 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     1801 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     1861 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     1921 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     1981 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     2041 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     2101 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     2161 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2221 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2281 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     2341 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     2401 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     2461 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     2521 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2581 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2641 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2701 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2761 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2821 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2881 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2941 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3001 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3061 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3121 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3181 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3241 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3421 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3481 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3541 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3601 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3661 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3721 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3781 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3841 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3901 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3961 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4021 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4081 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4141 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4201 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4261 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4321 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4381 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4441 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4501 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     4561 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     4621 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     4681 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     4741 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     4801 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     4861 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     4921 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     4981 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     5041 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     5101 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     5161 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     5221 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     5281 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
     5341 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagcttgg
     5401 taccgagctc ggatccccag cgtggacaat ggctactcaa gctgatttga tggagttgga
     5461 catggccatg gaaccagaca gaaaagcggc tgttagtcac tggcagcaac agtcttacct
     5521 ggactctgga atccattctg gtgccactac cacagctcct tctctgagtg gtaaaggcaa
     5581 tcctgaggaa gaggatgtgg atacctccca agtcctgtat gagtgggaac agggattttc
     5641 tcagtccttc actcaagaac aagtagctga tattgatgga cagtatgcaa tgactcgagc
     5701 tcagagggta cgagctgcta tgttccctga gacattagat gagggcatgc agatcccatc
     5761 tacacagttt gatgctgctc atcccactaa tgtccagcgt ttggctgaac catcacagat
     5821 gctgaaacat gcagttgtaa acttgattaa ctatcaagat gatgcagaac ttgccacacg
     5881 tgcaatccct gaactgacaa aactgctaaa tgacgaggac caggtggtgg ttaataaggc
     5941 tgcagttatg gtccatcagc tttctaaaaa ggaagcttcc agacacgcta tcatgcgttc
     6001 tcctcagatg gtgtctgcta ttgtacgtac catgcagaat acaaatgatg tagaaacagc
     6061 tcgttgtacc gctgggacct tgcataacct ttcccatcat cgtgagggct tactggccat
     6121 ctttaagtct ggaggcattc ctgccctggt gaaaatgctt ggttcaccag tggattctgt
     6181 gttgttttat gccattacaa ctctccacaa ccttttatta catcaagaag gagctaaaat
     6241 ggcagtgcgt ttagctggtg ggctgcagaa aatggttgcc ttgctcaaca aaacaaatgt
     6301 taaattcttg gctattacga cagactgcct tcaaatttta gcttatggca accaagaaag
     6361 caagctcatc atactggcta gtggtggacc ccaagcttta gtaaatataa tgaggaccta
     6421 tacttacgaa aaactactgt ggaccacaag cagagtgctg aaggtgctat ctgtctgctc
     6481 tagtaataag ccggctattg tagaagctgg tggaatgcaa gctttaggac ttcacctgac
     6541 agatccaagt caacgtcttg ttcagaactg tctttggact ctcaggaatc tttcagatgc
     6601 tgcaactaaa caggaaggga tggaaggtct ccttgggact cttgttcagc ttctgggttc
     6661 agatgatata aatgtggtca cctgtgcagc tggaattctt tctaacctca cttgcaataa
     6721 ttataagaac aagatgatgg tctgccaagt gggtggtata gaggctcttg tgcgtactgt
     6781 ccttcgggct ggtgacaggg aagacatcac tgagcctgcc atctgtgctc ttcgtcatct
     6841 gaccagccga caccaagaag cagagatggc ccagaatgca gttcgccttc actatggact
     6901 accagttgtg gttaagctct tacacccacc atcccactgg cctctgataa aggctactgt
     6961 tggattgatt cgaaatcttg ccctttgtcc cgcaaatcat gcacctttgc gtgagcaggg
     7021 tgccattcca cgactagttc agttgcttgt tcgtgcacat caggataccc agcgccgtac
     7081 gtccatgggt gggacacagc agcaatttgt ggagggggtc cgcatggaag aaatagttga
     7141 aggttgtacc ggagcccttc acatcctagc tcgggatgtt cacaaccgaa ttgttatcag
     7201 aggactaaat accattccat tgtttgtgca gctgctttat tctcccattg aaaacatcca
     7261 aagagtagct gcaggggtcc tctgtgaact tgctcaggac aaggaagctg cagaagctat
     7321 tgaagctgag ggagccacag ctcctctgac agagttactt cactctagga atgaaggtgt
     7381 ggcgacatat gcagctgctg ttttgttccg aatgtctgag gacaagccac aagattacaa
     7441 gaaacggctt tcagttgagc tgaccagctc tctcttcaga acagagccaa tggcttggaa
     7501 tgagactgct gatcttggac ttgatattgg tgcccaggga gaaccccttg gatatcgcca
     7561 ggatgatcct agctatcgtt cttttcactc tggtggatat ggccaggatg ccttgggtat
     7621 ggaccccatg atggaacatg agatgggtgg ccaccaccct ggtgctgact atccagttga
     7681 tgggctgcca gatctggggc atgcccagga cctcatggat gggctgcctc caggtgacag
     7741 caatcagctg gcctggtttg atactgacct ggacta
//
