LOCUS       sequence-316912-        9514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9514)
  AUTHORS   Zhang XY, La Russa VF, Reiser J
  TITLE     Transduction of bone-marrow-derived mesenchymal stem cells by using 
            lentivirus vectors pseudotyped with modified RD114 envelope 
            glycoproteins.
  JOURNAL   J Virol. 2004 Feb . 78(3):1219-29.
  PUBMED    14722277
REFERENCE   2  (bases 1 to 9514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2004 Feb . 78(3):1219-29."
COMMENT     Sequence Label: pCD/NL-BH*DDD
FEATURES             Location/Qualifiers
     source          1..9514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(33..50)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(204..792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(284..303)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(963..1754,1755..1823))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1586..1605
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1824..1928)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2003..2022)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2194..2573
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2574..2777
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2728..2748
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2774..2798
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2877..4379
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             4172..7183
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    6868..6985
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     rep_origin      7765..7900
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    8068..8301
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8486..8530
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8824..8850
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(9229..9246)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9235..9459
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
       61 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      121 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      181 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      241 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      301 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      361 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      421 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      481 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      541 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      601 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      661 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      721 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      781 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
      841 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
      901 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
      961 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1021 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1081 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     1141 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1201 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1261 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1321 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1381 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1441 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1501 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1561 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1621 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1681 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1741 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1801 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     1861 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     1921 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     1981 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     2041 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     2101 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     2161 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     2221 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     2281 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2341 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     2401 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     2461 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     2521 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     2581 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     2641 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     2701 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
     2761 tctatataag cagagctcgt ttagtgaacc gtcagatctc tagaagctct ctcgacgcag
     2821 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagagatgg
     2881 gtgcgagagc gtcggtatta agcgggggag aattagataa atgggaaaaa attcggttaa
     2941 ggccaggggg aaagaaacaa tataaactaa aacatatagt atgggcaagc agggagctag
     3001 aacgattcgc agttaatcct ggccttttag agacatcaga aggctgtaga caaatactgg
     3061 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacaa
     3121 tagcagtcct ctattgtgtg catcaaagga tagatgtaaa agacaccaag gaagccttag
     3181 ataagataga ggaagagcaa aacaaaagta agaaaaaggc acagcaagca gcagctgaca
     3241 caggaaacaa cagccaggtc agccaaaatt accctatagt gcagaacctc caggggcaaa
     3301 tggtacatca ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga
     3361 aggctttcag cccagaagta atacccatgt tttcagcatt atcagaagga gccaccccac
     3421 aagatttaaa taccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa
     3481 aagagaccat caatgaggaa gctgcagaat gggatagatt gcatccagtg catgcagggc
     3541 ctattgcacc aggccagatg agagaaccaa ggggaagtga catagcagga actactagta
     3601 cccttcagga acaaatagga tggatgacac ataatccacc tatcccagta ggagaaatct
     3661 ataaaagatg gataatcctg ggattaaata aaatagtaag aatgtatagc cctaccagca
     3721 ttctggacat aagacaagga ccaaaggaac cctttagaga ctatgtagac cgattctata
     3781 aaactctaag agccgagcaa gcttcacaag aggtaaaaaa ttggatgaca gaaaccttgt
     3841 tggtccaaaa tgcgaaccca gattgtaaga ctattttaaa agcattggga ccaggagcga
     3901 cactagaaga aatgatgaca gcatgtcagg gagtgggggg acccggccat aaagcaagag
     3961 ttttggctga agcaatgagc caagtaacaa atccagctac cataatgata cagaaaggca
     4021 attttaggaa ccaaagaaag actgttaagt gtttcaattg tggcaaagaa gggcacatag
     4081 ccaaaaattg cagggcccct aggaaaaagg gctgttggaa atgtggaaag gaaggacacc
     4141 aaatgaaaga ttgtactgag agacaggcta attttttagg gaagatctgg ccttcccaca
     4201 agggaaggcc agggaatttt cttcagagca gaccagagcc aacagcccca ccagaagaga
     4261 gcttcaggtt tggggaagag acaacaactc cctctcagaa gcaggagccg atagacaagg
     4321 aactgtatcc tttagcttcc ctcagatcac tctttggcag cgacccctcg tcacaataaa
     4381 gatagggggg caattaaagg aagctctatt agatacagga gcagatgata cagtattaga
     4441 agaaatgaat ttgccaggaa gatggaaacc aaaaatgata gggggaattg gaggttttat
     4501 caaagtaaga cagtatgatc agatactcat agaaatctgc ggacataaag ctataggtac
     4561 agtattagta ggacctacac ctgtcaacat aattggaaga aatctgttga ctcagattgg
     4621 ctgcacttta aattttccca ttagtcctat tgagactgta ccagtaaaat taaagccagg
     4681 aatggatggc ccaaaagtta aacaatggcc attgacagaa gaaaaaataa aagcattagt
     4741 agaaatttgt acagaaatgg aaaaggaagg aaaaatttca aaaattgggc ctgaaaatcc
     4801 atacaatact ccagtatttg ccataaagaa aaaagacagt actaaatgga gaaaattagt
     4861 agatttcaga gaacttaata agagaactca agatttctgg gaagttcaat taggaatacc
     4921 acatcctgca gggttaaaac agaaaaaatc agtaacagta ctggatgtgg gcgatgcata
     4981 tttttcagtt cccttagata aagacttcag gaagtatact gcatttacca tacctagtat
     5041 aaacaatgag acaccaggga ttagatatca gtacaatgtg cttccacagg gatggaaagg
     5101 atcaccagca atattccagt gtagcatgac aaaaatctta gagcctttta gaaaacaaaa
     5161 tccagacata gtcatctatc aatacatgga tgatttgtat gtaggatctg acttagaaat
     5221 agggcagcat agaacaaaaa tagaggaact gagacaacat ctgttgaggt ggggatttac
     5281 cacaccagac aaaaaacatc agaaagaacc tccattcctt tggatgggtt atgaactcca
     5341 tcctgataaa tggacagtac agcctatagt gctgccagaa aaggacagct ggactgtcaa
     5401 tgacatacag aaattagtgg gaaaattgaa ttgggcaagt cagatttatg cagggattaa
     5461 agtaaggcaa ttatgtaaac ttcttagggg aaccaaagca ctaacagaag tagtaccact
     5521 aacagaagaa gcagagctag aactggcaga aaacagggag attctaaaag aaccggtaca
     5581 tggagtgtat tatgacccat caaaagactt aatagcagaa atacagaagc aggggcaagg
     5641 ccaatggaca tatcaaattt atcaagagcc atttaaaaat ctgaaaacag gaaagtatgc
     5701 aagaatgaag ggtgcccaca ctaatgatgt gaaacaatta acagaggcag tacaaaaaat
     5761 agccacagaa agcatagtaa tatggggaaa gactcctaaa tttaaattac ccatacaaaa
     5821 ggaaacatgg gaagcatggt ggacagagta ttggcaagcc acctggattc ctgagtggga
     5881 gtttgtcaat acccctccct tagtgaagtt atggtaccag ttagagaaag aacccataat
     5941 aggagcagaa actttctatg tagatggggc agccaatagg gaaactaaat taggaaaagc
     6001 aggatatgta actgacagag gaagacaaaa agttgtcccc ctaacggaca caacaaatca
     6061 gaagactgag ttacaagcaa ttcatctagc tttgcaggat tcgggattag aagtaaacat
     6121 agtgacagac tcacaatatg cattgggaat cattcaagca caaccagata agagtgaatc
     6181 agagttagtc agtcaaataa tagagcagtt aataaaaaag gaaaaagtct acctggcatg
     6241 ggtaccagca cacaaaggaa ttggaggaaa tgaacaagta gataaattgg tcagtgctgg
     6301 aatcaggaaa gtactatttt tagatggaat agataaggcc caagaagaac atgagaaata
     6361 tcacagtaat tggagagcaa tggctagtga ttttaaccta ccacctgtag tagcaaaaga
     6421 aatagtagcc agctgtgata aatgtcagct aaaaggggaa gccatgcatg gacaagtaga
     6481 ctgtagccca ggaatatggc agctagattg tacacattta gaaggaaaag ttatcttggt
     6541 agcagttcat gtagccagtg gatatataga agcagaagta attccagcag agacagggca
     6601 agaaacagca tacttcctct taaaattagc aggaagatgg ccagtaaaaa cagtacatac
     6661 agacaatggc agcaatttca ccagtactac agttaaggcc gcctgttggt gggcggggat
     6721 caagcaggaa tttggcattc cctacaatcc ccaaagtcaa ggagtaatag aatctatgaa
     6781 taaagaatta aagaaaatta taggacaggt aagagatcag gctgaacatc ttaagacagc
     6841 agtacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     6901 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     6961 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     7021 gaaaggacca gcaaagctcc tctggaaagg tgaaggggca gtagtaatac aagataatag
     7081 tgacataaaa gtagtgccaa gaagaaaagc aaagatcatc agggattatg gaaaacagat
     7141 ggcaggtgat gattgtgtgg caagtagaca ggatgaggat taacacatgg aaaagattag
     7201 taaaataata agaattctgc aacaactgct gtttatccat ttcagaattg ggtgtcgaca
     7261 tagcagaata ggcgttactc gacagaggag agcaagaaat ggagccagta gatcctagac
     7321 tagagccctg gaagcatcca ggaagtcagc ctaaaactgc ttgtaccaat tgctattgta
     7381 aaaagtgttg ctttcattgc caagtttgtt tcatgacaaa agccttaggc atctcctatg
     7441 gcaggaagaa gcggagacag cgacgaagag ctcatcagaa cagtcagact catcaagctt
     7501 ctctatcaaa gcagtaagta gtacatgtaa tttaattgat agactaatag aaagagcaga
     7561 agacagtggc aatgagagtg aaggagaagt atcagcactt gtggagatgg gggtggaaat
     7621 ggggcaccat gctccttggg atattgatga tctgtagtgc tacagaaaaa ttgtgggtca
     7681 cagtctatta tggggtacct gtgtggaagg aagcaaccac cactctattt tgtgcatcag
     7741 atgctaaagc atatgactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7801 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7861 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaatgcata
     7921 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     7981 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     8041 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     8101 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     8161 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     8221 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     8281 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     8341 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaataacat
     8401 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     8461 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     8521 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     8581 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctgtagtgaa
     8641 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa tcccgagggg
     8701 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     8761 tcgattagtg aacggatcct tggcacttat ctgggacgat ctgcggagcc tgtgcctctt
     8821 cagctaccac cgcttgagag acttactctt gattgtaacg aggattgtgg aacttctggg
     8881 acgcaggggg tgggaagccc tcaaatattg gtggaatctc ctacagtatt ggagtcagga
     8941 actaaagaat agtgctgtta gcttgctcaa tgccacagcc atagcagtag ctgaggggac
     9001 agatagggtt atagaagtag tacaaggagc ttgtagagct attcgccaca tacctagaag
     9061 aataagacag ggcttggaaa ggattttgct ataagatggg tggcaagtgg tcaaaaagta
     9121 gtgtgattgg atggcctact gtaagggaaa gaatgagacg agctgagcca gcagcagata
     9181 gggtgggagc agcatctcga gtctagagcg tttaaacccg ctgatcagcc tcgactgtgc
     9241 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     9301 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     9361 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     9421 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     9481 gctgcattaa tgaatcggcc aacgcgcggg gaga
//
