LOCUS       sequence-316916-        6443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of human Elk-1 with an HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Lee SM, Vasishtha M, Prywes R
  TITLE     Activation and repression of cellular immediate early genes by serum
            response factor cofactors.
  JOURNAL   J Biol Chem. 2010 Jul 16. 285(29):22036-49.
  PUBMED    20466732
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Jul 16. 285(29):22036-49."
COMMENT     Sequence Label: pCGN-ELK-1
FEATURES             Location/Qualifiers
     source          1..6443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..46)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(200..788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(280..299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(959..1750,1751..1819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1582..1601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1820..1924)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1992..2010
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2048..2070)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2170..2189
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2256..2711)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2393..2414)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2605..2624
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2837..2859
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2851..2868
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2852..2868
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2875..2894
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2875..2893
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        2912..3215
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3216..3419
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3370..3390
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3416..3440
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           3509..3548
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             3578..3604
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3584..3610
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     intron          5053..5625
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5757..5776)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5822..5877
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5876..5895)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      6231..6366
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
       61 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1921 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     1981 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     2041 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     2101 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     2161 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     2221 cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt
     2281 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     2341 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     2401 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     2461 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     2521 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     2581 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     2641 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     2701 acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2761 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2821 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
     2881 gactcactat agggcgaatt cgagctcgcc ccgttacata acttacggta aatggcccgc
     2941 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     3001 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     3061 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     3121 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     3181 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     3241 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     3301 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     3361 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     3421 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
     3481 gacaccggga ccgatccagc ctccggggga tcttggtggc gtgaaactcc cgcacctctt
     3541 cggccagcgc cttgtagaag cgcgtatggc ttctagctat ccttatgacg tgcctgacta
     3601 tgccagcctg ggaggacctt ctagaatgga cccatctgtg acgctgtggc agtttctgct
     3661 gcagctgctg agagagcaag gcaatggcca catcatctcc tggacttcac gggatggtgg
     3721 tgaattcaag ctggtggatg cagaggaggt ggcccggctg tggggactac gcaagaacaa
     3781 gaccaacatg aattacgaca agctcagccg ggccttgcgg tactactatg acaagaacat
     3841 catccgcaag gtgagcggcc agaagttcgt ctacaagttt gtgtcctacc ctgaggtcgc
     3901 agggtgctcc actgaggact gcccgcccca gccagaggtg tctgttacct ccaccatgcc
     3961 aaatgtggcc cctgctgcta tacatgccgc cccaggggac actgtctctg gaaagccagg
     4021 cacacccaag ggtgcaggaa tggcaggccc aggcggtttg gcacgcagca gccggaacga
     4081 gtacatgcgc tcgggcctct attccacctt caccatccag tctctgcagc cgcagccacc
     4141 ccctcatcct cggcctgctg tggtgctccc caatgcagct cctgcagggg cagcagcgcc
     4201 cccctcgggg agcaggagca ccagtccaag ccccttggag gcctgtctgg aggctgaaga
     4261 ggccggcttg cctctgcagg tcatcctgac cccgcccgag gccccaaacc tgaaatcgga
     4321 agagcttaat gtggagccgg gtttgggccg ggctttgccc ccagaagtga aagtagaagg
     4381 gcccaaggaa gagttggaag ttgcggggga gagagggttt gtgccagaaa ccaccaaggc
     4441 cgagccagaa gtccctccac aggagggcgt gccagcccgg ctgcccgcgg ttgttatgga
     4501 caccgcaggg caggcgggcg gccatgcggc ttccagccct gagatctccc agccgcagaa
     4561 gggccggaag ccccgggacc tagagcttcc actcagcccg agcctgctag gtgggccggg
     4621 acccgaacgg accccaggat cgggaagtgg ctccggcctc caggctccgg ggccggcgct
     4681 gaccccatcc ctgcttccta cgcatacatt gaccccggtg ctgctgacac ccagctcgct
     4741 gcctcctagc attcacttct ggagcaccct gagtcccatt gcgccccgta gcccggccaa
     4801 gctctccttc cagtttccat ccagtggcag cgcccaggtg cacatccctt ctatcagcgt
     4861 ggatggcctc tcgacccccg tggtgctctc cccagggccc cagaagccat gactactacc
     4921 accaccacca ccaccccttc tggggtcact ccatccatgc tctctccagc cagccatctc
     4981 aaggagaaac atagttcaac tgaaagactc atgctctgat tgtggtgggg tggggatcct
     5041 gagaacttca gggtgagttt ggggaccctt gattgttctt tctttttcgc tattgtaaaa
     5101 ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg aagatgtccc
     5161 ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact ctgttgacaa
     5221 ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa ctttagcttg
     5281 catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa gtactttctc
     5341 taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc acagttttag
     5401 agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa ttctggctgg
     5461 cgtggaaata ttcttattgg tagaaacaac tacaccctgg tcatcatcct gcctttctct
     5521 ttatggttac aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg
     5581 ggcccctctg ctaaccatgt tcatgccttc ttctctttcc tacagctcct gggcaacgtg
     5641 ctggttgttg tgctgtctca tcattttggc aaagaattca ctcctcaggt gcaggctgcc
     5701 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     5761 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     5821 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     5881 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     5941 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     6001 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     6061 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     6121 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     6181 gtccctcttc tcttatggag atccctcgag gagctttttg caaaagccta ggcctccaaa
     6241 aaagcctctt cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
     6301 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     6361 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgctgc attaatgaat
     6421 cggccaacgc gcggggagag gcg
//
