LOCUS       sequence-316917-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses murine IFN gamma in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     Expression plasmid for production of murine IFN gamma
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mu-IFNg-pCIneo
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          140..272
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     255..274
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        317..335
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagaagct ttattgcggt agtttatcac agttaaattg ctaacgcagt cagtgcttct
       61 gacacaacag tctcgaactt aagctgcagt gactctctta aggtagcctt gcagaagttg
      121 gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
      181 gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct
      241 tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc
      301 ttaaggctag agtacttaat acgactcact ataggctagc ctcgagagga tccaccgcgg
      361 ccaggaccat gaacgctaca cactgcatct tggctttgca gctcttcctc atggctgttt
      421 ctggctgtta ctgccacggc acagtcattg aaagcctaga aagtctgaat aactatttta
      481 actcaagtgg catagatgtg gaagaaaaga gtctcttctt ggatatctgg aggaactggc
      541 aaaaggatgg tgacatgaaa atcctgcaga gccagattat ctctttctac ctcagactct
      601 ttgaagtctt gaaagacaat caggccatca gcaacaacat aagcgtcatt gaatcacacc
      661 tgattactac cttcttcagc aacagcaagg cgaaaaagga tgcattcatg agtattgcca
      721 agtttgaggt caacaaccca caggtccagc gccaagcatt caatgagctc atccgagtgg
      781 tccaccagct gttgccggaa tccagcctca ggaagcggaa aaggagtcgc tgctgattcg
      841 tcgcgactct aga
//
