LOCUS       sequence-316919-        5226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Hornung V, Ablasser A, Charrel-Dennis M, Bauernfeind F, Horvath G, 
            Caffrey DR, Latz E, Fitzgerald KA
  TITLE     AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating 
            inflammasome with ASC.
  JOURNAL   Nature. 2009 Mar 26. 458(7237):514-8.
  PUBMED    19158675
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Mar 26. 458(7237):514-8."
COMMENT     Sequence Label: pCI-Caspase 1
FEATURES             Location/Qualifiers
     source          1..5226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     73..94
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(298..317)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     417..439
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(477..495)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        563..667
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(668..736,737..1528)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(886..905)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1699..2287
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2188..2207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2498..2877
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2878..3081
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3032..3052
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3078..3102
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          3217..3349
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3332..3351
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        3394..3412
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4644..4673
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    4700..4821
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4711..4730)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4765..4784
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      join(5002..5226,1..231)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5089..5108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
       61 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
      121 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
      181 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg
      241 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
      301 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
      361 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
      421 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
      481 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
      541 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
      601 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      661 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      721 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
      781 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
      841 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
      901 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      961 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     1021 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     1081 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     1141 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     1201 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     1261 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     1321 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     1381 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     1441 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     1501 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     1561 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     1621 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1681 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1741 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1801 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1861 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1921 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1981 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2041 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2101 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2161 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2221 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2281 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2341 tcacatggct cgacagatct tcaatattgg ccattagcca tattattcat tggttatata
     2401 gcataaatca atattggcta ttggccattg catacgttgt atctatatca taatatgtac
     2461 atttatattg gctcatgtcc aatatgaccg ccatgttggc attgattatt gactagttat
     2521 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     2581 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     2641 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     2701 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg
     2761 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     2821 ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     2881 atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca
     2941 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     3001 ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg
     3061 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcactagaag ctttattgcg
     3121 gtagtttatc acagttaaat tgctaacgca gtcagtgctt ctgacacaac agtctcgaac
     3181 ttaagctgca gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag
     3241 gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga
     3301 taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc
     3361 cagttcaatt acagctctta aggctagagt acttaatacg actcactata ggctagcctc
     3421 gagaattcgc catggccgac aaggtcctga aggagaagag aaagctgttt atccgttcca
     3481 tgggtgaagg tacaataaat ggcttactgg atgaattatt acagacaagg gtgctgaaca
     3541 aggaagagat ggagaaagta aaacgtgaaa atgctacagt tatggataag acccgagctt
     3601 tgattgactc cgttattccg aaaggggcac aggcatgcca aatttgcatc acatacattt
     3661 gtgaagaaga cagttacctg gcagggacgc tgggactctc agcagatcaa acatctggaa
     3721 attaccttaa tatgcaagac tctcaaggag tactttcttc ctttccagct cctcaggcag
     3781 tgcaggacaa cccagctatg cccacatcct caggctcaga agggaatgtc aagctttgct
     3841 ccctagaaga agctcaaagg atatggaaac aaaagtcggc agagatttat ccaataatgg
     3901 acaagtcaag ccgcacacgt cttgctctca ttatctgcaa tgaagaattt gacagtattc
     3961 ctagaagaac tggagctgag gttgacatca caggcatgac aatgctgcta caaaatctgg
     4021 ggtacagcgt agatgtgaaa aaaaatctca ctgcttcgga catgactaca gagctggagg
     4081 catttgcaca ccgcccagag cacaagacct ctgacagcac gttcctggtg ttcatgtctc
     4141 atggtattcg ggaaggcatt tgtgggaaga aacactctga gcaagtccca gatatactac
     4201 aactcaatgc aatctttaac atgttgaata ccaagaactg cccaagtttg aaggacaaac
     4261 cgaaggtgat catcatccag gcctgccgtg gtgacagccc tggtgtggtg tggtttaaag
     4321 attcagtagg agtttctgga aacctatctt taccaactac agaagagttt gaggatgatg
     4381 ctattaagaa agcccacata gagaaggatt ttatcgcttt ctgctcttcc acaccagata
     4441 atgtttcttg gagacatccc acaatgggct ctgtttttat tggaagactc attgaacata
     4501 tgcaagaata tgcctgttcc tgtgatgtgg aggaaatttt ccgcaaggtt cgattttcat
     4561 ttgagcagcc agatggtaga gcgcagatgc ccaccactga aagagtgact ttgacaagat
     4621 gtttctacct cttcccagga catgaacaga aattgatctc cgaagaagac ttgtaggcgg
     4681 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     4741 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     4801 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     4861 ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     4921 aaggatccgg gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4981 cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5041 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5101 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5161 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5221 gtgatg
//
