LOCUS       sequence-31692-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty backbone for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown 
            NA, Gileadi O
  TITLE     High-throughput production of human proteins for crystallization: 
            the SGC experience.
  JOURNAL   J Struct Biol. 2010 Oct;172(1):3-13. doi: 10.1016/j.jsb.2010.06.008.
            Epub 2010 Jun 10.
  PUBMED    20541610
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jsb.2010.06.008"; journalName: "J Struct Biol"; date: 
            "2010-10"; volume: "172"; issue: "1"; pages: "3-13"
COMMENT     Sequence Label: pNIC-CTHF
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             710..730
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             731..748
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             749..772
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             822..839
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tacttagtat ttgaagcaaa cactggaact gaagatggct accaaggcga agaatcttta
       61 tttaacaaag catactatgg caaaagcaca tcattcttcc gtcaagaaag tcaaaaactt
      121 ctgcaaagcg ataaaaaacg tacggctgag ttagcaaacg gcgctctcgg tatgattgag
      181 ctaaacgatg attacacact gaaaaaagtg atgaaaccgc tgattgcatc taacacagta
      241 acagatgaaa ttgaacgcgc gaacgtcttt aaaatgaacg gcaaatggta cctgttcact
      301 gactcccgcg gatcaaaaat gacgattgac ggcattacgt ctaacgatat ttacatgctt
      361 ggttatgttt ctaattcttt aactggccca tacaagccgc tgaacaaaac tggccttgtg
      421 ttaaaaatgg atcttgatcc taacgatgta acctttactt actcacactt cgctgtacct
      481 caagcgaaag gaaacaatgt cgtgattaca agctatatga caaacagagg attctacgca
      541 gacaaacaat caacgtttgc gcctagcttc ctgctgaaca tcaaaggcaa gaaaacatct
      601 gttgtcaaag acagcatcct tgaacaagga caattaacag ttaacaaata aaaacgcaaa
      661 agaaaatgcc gatatcctat tggcattgac gtcaggtggc acacctgcag agaacctcta
      721 cttccaatcg caccatcatc accaccatga ttacaaggat gacgacgata agtgaggatc
      781 cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccac
//
