LOCUS       sequence-316921-       11623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of xylose utilization genes of Yarrowia lipolytica 
            using EasyCloneYALI. Cut with NotI to linearize repair fragment for 
            C3 locus, overexpressing XK, XDH, and XR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Konzock O, Zaghen S, Norbeck J
  TITLE     Tolerance of Yarrowia lipolytica to inhibitors commonly found in 
            lignocellulosic hydrolysates.
  JOURNAL   BMC Microbiol. 2021 Mar 8;21(1):77. doi: 10.1186/s12866-021-02126-0.
  PUBMED    33685391
REFERENCE   2  (bases 1 to 11623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2021-03-8"; volume: "21(1):77. doi"; pages: " 
            10.1186/s12866-021-02126-0"
COMMENT     Sequence Label: SZ_EC_02
FEATURES             Location/Qualifiers
     source          1..11623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(3532..3565)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(3772..3791)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(3797..3816)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        3858..3949
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3950..4018,4019..4810)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4168..4187)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4932..5520
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5421..5440
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5674..5691
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     5789..5806
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5790..5806
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6276..6309
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     RBS             10908..10919
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggttgaaatg aatcggccga cgctcggtag tcggaaagag ccgggaccgg ccggcgagca
       61 taaaccggac gcagtaggat gtcctgcacg ggtctttttg tggggtgtgg agaaaggggt
      121 gcttggagat ggaagccggt agaaccgggc tgcttggggg gatttggggc cgctgggctc
      181 caaagagggg taggcatttc gttggggtta cgtaattgcg gcatttgggt cctgcgcgca
      241 tgtcccattg gtcagaatta gtccggatag gagacttatc agccaatcac agcgccggat
      301 ccacctgtag gttgggttgg gtgggagcac ccctccacag agtagagtca aacagcagca
      361 gcaacatgat agttgggggt gtgcgtgtta aaggaaaaaa aaagaagctt gggttatatt
      421 cccgctctat ttagaggttg cgggatagac gccgacggag ggcaatggcg ccatggaacc
      481 ttgcggatat cgatacgccg cggcggactg cgtccgagcc agctccagca gcgttttttc
      541 cgggccattg agccgactgc gaccccgcca acgtgtcttg gcccacgcac tcatgtcatg
      601 ttggtgttgg gaggccactt tttaagtagc acaaggcacc tagctcgcag caaggtgtcc
      661 gaaccaaaga agcggctgca gtggtgcaaa cggggcggaa acggcgggaa aaagccacgg
      721 gggcacgaat tgaggcacgc cctcgaattt gagacgagtc acggccccat tcgcccgcgc
      781 aatggctcgc caacgcccgg tcttttgcac cacatcaggt taccccaagc caaacctttg
      841 tgttaaaaag cttaacatat tataccgaac gtaggtttgg gcgggcttgc tccgtctgtc
      901 caaggcaaca tttatataag ggtctgcatc gccggctcaa ttgaatcttt tttcttcttc
      961 tcttctctat attcattctt gaattaaaca cacatcaaca atgtcttcta acctgtcatt
     1021 tgttcttcga aagccattgg atctcgtctt tgaggatcgg cccgacccca agatccagga
     1081 cccccactcc gtcaaggtgg cagtcaaaaa gaccggagtt tgcggctcgg atgtccacta
     1141 ctatctgcat ggaggaatcg gcgacttcat tgtcaaggct cccatggttc taggccatga
     1201 aagtgccgga gaggtggttg aggttggtcc tgaagtcaag gacctcaagg tgggagatcg
     1261 agtggctctc gagcccggag tgccgtctcg attgtcacag gagtacaagg agggacgata
     1321 caacctgtgt ccttgcatgg tgtttgctgc cacccctccc tacgacggta ctctgtgtcg
     1381 tcactacatc attcccgagg acttttgtgt caagctgcct gatcatgtgt ctctcgagga
     1441 gggagctctt gtggagcctc tgtccgtggc tgtccactgc aacaagctgg ccaagaccac
     1501 tgcccaggac gtggttattg tatttggagc tggcccagtc ggactgctag ccgtgggagt
     1561 ggccaatgcc tttggatcat ctaccattgt gtgtgttgat cttgttcccg agaagctgga
     1621 gctcgccaag aagttcggtg ccactcatac gtttgtaccc actaagggag acagtcccaa
     1681 cgagtctgct gacaagatcc gagctctgat caagggcgct ggtctctctg actcgcccaa
     1741 tgtggctttg gagtgcaccg gagctgagcc ttctattcag actgctgttt ctgtgctggc
     1801 cacttccggt cgacttgtgc aggtcggcat gggcaaggat gacgtcaact tccctatcac
     1861 caaatgcatt gtaaaggaga ttaccgtgct cggatcgttc cgatactgcc atggtgacta
     1921 tcccctggct gttcagctgg ttgcttctgg caagattgac gtcaagaagc tggtgaccaa
     1981 ccggttcacc ttcaaggagg ctgagcaggc gtacaagacg gcggccgagg gcaaggccat
     2041 caagatcatc attgacggtc ccgaggagga gtagtacgcg tgcattcctt ctgttcggaa
     2101 tcaacctcaa ggttaacggc cacgatcccc tcgttgttac tcttggtcag cccattgtcg
     2161 gtaacgctgg ctttgctaac tgggtcgata aactcttctt tggccaggag aaccccgatg
     2221 tctccaaggt gtccaaagac cgaaagctct accgaatcac ccaccgagga gatatcgtcc
     2281 ctcaagtgcc cttctgggac ggttaccagc actgctctgg tgaggtcttt attgactggc
     2341 ccctgatcca ccctcctctc tccaacgttg tcatgtgcca gggccagagc aataaacagt
     2401 gctctgccgg taacactctg ctccagcagg tcaatgtgat tggaaaccat ctgcagtact
     2461 tcgtcaccga gggtgtctgt ggtatctaag ctatttatca ctctttacaa cttctacctc
     2521 aactatctac tttaataaat gaatatcgtt tattctctat gattactgta tatgcgttcc
     2581 tctaagacaa atcgaaacca gcatgcgatc gaatggcata caaaagtttc ttccgaagtt
     2641 gatcaatgtc ctgatagtca ggcagcttga gaagattgac acaggtggag gccgtaggga
     2701 accgatcaac ctgtctacca gcgttacgaa tggcaaatga cgggttcaaa gccttgaatc
     2761 cttgcaatgg tgccttggat actgatgtca caaacttaag aagcagccgc ttgtcctctt
     2821 cctcgaaact ctcaaacaca gtccagaagt cctttatagt ttgatctgta tccagatagc
     2881 ctccgtaatt ggtgtgtgtc ttcaaatccc agacgtccac attggcatgt cctccactga
     2941 taagcatttg aagttcatct gcgttgaaca ttgagaccca cgaagggtca atgagctggt
     3001 atagaccgcc caagaatgca tctgagtggc ctgccatagc actattgtag agtggccatt
     3061 gttcgtgtcg atccgtacga cgatcatgga gcgagcggcc acgaaattag atagccacta
     3121 aaactagtcc tgtccacgga aggccgtttg ttagccgctt atatcgttgg aacaatactt
     3181 ttgttgccgg catctaattt cataatttcg tgattaattt ttcccgccaa aaaataaaaa
     3241 atcaaaaaca aaaaataaaa aggaactccg gtaagagcgc tcccgaaacc ggaaatgcat
     3301 ggtttctctc atgtgtgcct cttgcttgtc tcaggccggt ccagaaacaa acctcgagct
     3361 tccgcctcca agaccctgct ttcggagaag cagccaatgg ctgttgtttg tcactgttcc
     3421 ctgtcttggt ttttttggcc cttcttttct tcttcccccc atgaaacctt gtgtgtgtcc
     3481 tgtgcaataa ttatcgcgtg cattcgcggc cgcatttaaa tccccacttc agaagttcct
     3541 atactttcta gagaatagga acttcactat agagtcgaat aagggcgaca ccccataatt
     3601 agcccgggcg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcag
     3661 ttccctactc tcgcatgggg agtccccaca ctaccatcgg cgctacggcg tttcacttct
     3721 gagttcggca tggggtcagg tgggaccacc gcgctactgc cgccaggcaa acaaggggtg
     3781 ttatgagcca tattcaggta taaatgggct cgcgataatg ttcagaattg gttaattggt
     3841 tgtaacactg acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3901 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagaata tgagtattca
     3961 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4021 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4081 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4141 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4201 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4261 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4321 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4381 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4441 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcgat
     4501 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4561 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4621 ggctggctgg tttattgctg ataaatccgg agccggtgag cgtggttctc gcggtatcat
     4681 cgcagcgctg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4741 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4801 gcattggtaa gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     4861 cccttaacgt gagttacgcg cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     4921 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4981 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5041 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5101 gcccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5161 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5221 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5281 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5341 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5401 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5461 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5521 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5581 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5641 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     5701 ggcgagagta gggaactgcc aggcatcaaa ctaagcagaa ggcccctgac ggatggcctt
     5761 tttgcgtttc tacaaactct ttctgtgttg taaaacgacg gccagtctta agctcgggcc
     5821 ccctgggcgg ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc
     5881 ggcgggtttt tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaagggcg
     5941 cctgtcactt tgcttgatat atgagaatta tttaacctta taaatgagaa aaaagcaacg
     6001 cactttaaat aagatacgtt gctttttcga ttgatgaaca cctataatta aactattcat
     6061 ctattattta tgattttttg tatatacaat atttctagtt tgttaaagag aattaagaaa
     6121 ataaatctcg aaaataataa agggaaaatc agtttttgat atcaaaatta tacatgtcaa
     6181 cgataataca aaatataata caaactataa gatgttatca gtatttatta tgcatttaga
     6241 ataaattttg tgtcgccctt attcgactca ctatagaagt tcctattctc tagaaagtat
     6301 aggaacttca cttcatttta tttaaatttg cggccgcgaa tgcgtgcgat aaaaaactgt
     6361 agtagtgtgg tgatggagtc ataacccgct ccggctatgt gcggatacag tattgtctca
     6421 gacagtggtc gcgagcaatg tcttgtgcat cttcatactt tttttttttt tattttgagt
     6481 ggggaggttg aggttgggtc agaggttggg tcatccatga agaggttaac agaacgagcc
     6541 acgatagggg gaaggatctt caactcgatc cgctattgtt ctcagtgatg gcaaatagat
     6601 tcatctaaga cctaccggta caagtaaagt acagcacacg taatttttct tgcggctagt
     6661 ctactctcag tgcatgtaac gtgcggatct agcccatgaa caggtggttt ttaccctcct
     6721 gctctccaca tttccagata gatgcaggct tctatggttg ttccacactc ttgtcagagg
     6781 agatgcccgt atacgaccac cataagcgac cctattacgg ggcccgaaca agccccgttg
     6841 atagacactg taacgtgcaa cgcttacgca actaacatga atgaatacga tatacatcaa
     6901 agactatgat acgcagtatt gcacactgta cgagtaaggg cactagccac tgcactcaag
     6961 tgaaaccgtt gcccgggtac gagtatgagt atgtacagta tgtttagtat tgtacttgga
     7021 caatgcttgt atcgtacatt ctcaagtgtc aaacataaat atccgttgct atatcctcgc
     7081 accaccacgt agctcgctat atccctgtgt tgaatccatc catcttggat tgccaattgt
     7141 gcacacagaa ccgggcactc acttccccat ccacacttca acggaatgcg tgcgatttat
     7201 ttctccaggc aggcgttttc ggccagcttg aacgccttga gtagcacgtc tgtgtccaca
     7261 ttcttgtatt tgacctcttc ttcgtgaacc tgtacctggc ctgtcttgtt ggggtcgcaa
     7321 agccaggcgt gatgggcact tccaatggca caagcgtttg ccatttccag accacatctc
     7381 caagcgccct ttgttggttt taagatggca gacattacgc tgcagatagc agtgttgacc
     7441 gaggcgcctc ccacaaaata cacctttcca atatcgccat gcatcatgcg ttggagtctc
     7501 attttgatac tgagggcctg gctttgaatg atggcagtgg cgtccatttg tggcggccac
     7561 gtgtccttga gattttgagc ctgcggtttg tgttcggttg ccagtgagtc ctctggagaa
     7621 gccatctctg taagctgttc actttgtctg ttgtagatga aacgtcgttc aaagggacct
     7681 gctcgaggaa ggatttctcg tagtggaaag tagatgccaa catggacatc gtcagcagac
     7741 actgtgttgg tggtctcggc cgcctcatta aactcgtccc agcctccaag ctcgtctcgg
     7801 atcttctctc gtgctagacc tccgttgcag tagcacagca tgcccatgta tccctccatg
     7861 gggtggttga acacatggta tccggggtcg ggcatgtact tgtttgttac gacgagggcg
     7921 gtcgtagatg ttcccaaaga cacaatcacg tcattggcct gtaaggggag agccagcata
     7981 gtgcctgggt tgtcgcctgt gaactgtgcc acctgacaag agggagagaa gcccttggag
     8041 acgaaatagg gcgagatttt gcccagtaga gccttggggt ctgtttccac gtctccaagg
     8101 agggactcga ccgagtgggt gtcatcggtg atgagatctg tgagtttctt gcaccactgg
     8161 ccattctgga tgtcccacag attcattcca caggcgtcag caagatcaaa ggggcacgcc
     8221 ttgccggcaa acagagatgc tagaaagttg gatatgagct ggacccgtga cgtggccttg
     8281 aataccttgg ggttgcgttc cttgaccttg agaatctggg gcccggtgaa acgagtatgt
     8341 gctcgagagc cagtgatctc agccagctgc tcgggaccgc cgactgcagc ctcaaactgc
     8401 gcacactcct tctccgtgct tgcatcctgc caattggggc tgacttcgag cgccagtaag
     8461 gatgacactc ctgtagacaa gtctttgtgt ttgtccagag aacccaggcc ctccttgtag
     8521 ctgctgttga gataaacaga gccgtgttgc tggcacgagc ccgacacaaa cttgatcgtg
     8581 gaaaggtcta tttcctttga cagacgctct atcagcaagt ccacagctgc cccccacatt
     8641 gccacaggag cattgatttc tccggccact gtagagctgt gaaagacgcc cttttcggtg
     8701 ttgaactgcg gcaagtcgtc ctcaaagtcc acatggactt gtgtgaccgt gtccagcgtt
     8761 tttgtgtcca gaatgatgcc cttgagctgt tgagtcgaaa gatccagtcc gagatacatt
     8821 ttgaatgatt cttatactca gaaggaaatg cttaacgatt tcgggtgtga gttgacaagg
     8881 agagagagaa aagaagagga aaggtaattc ggggacggtg gtcttttata cccttggctt
     8941 aagtcccaac cacaaagcaa aaaaattttc agtagtctat tttgcgtccg gcatgggtta
     9001 cccggatggc cagacaaaga aactagtaca aagtctgaac aagcgtagat tccagactgc
     9061 agtaccctac gcccttaacg gcaagtgtgg gaaccggggg aggtttgata tgtggggtga
     9121 agggggctct cgccggggtt gggtccgcta ctgggtcaat ttggggtcaa ttggggcaat
     9181 tggggctgtt ttttgggaca caaatgcgcc gccaacccgg tctctgcgat aagcttcgag
     9241 aagcccgaac tcgagccaag ggttctggtt ttgatgatct ggtcgaggct gctgacggag
     9301 agctcaacaa ggttgatggt ctggcgcgaa aggcgttgaa acagaatgag ccagacagca
     9361 aggacaaggt ggccaacagc aaggagtcca aaaagccctc tattgacgag atccacgatg
     9421 ttattgctca tgaggtttcc gagctcgatg ctgggaagaa gaagtgattt gtatataaga
     9481 aataaatgag atatagtaaa ggagtgcaag agaatggcaa ggtggtcaaa ttctatatta
     9541 cttgcagtca ctggttcctc gttgacatga atgaagttac cgttggcata gctgatttaa
     9601 tatataactg tccaactaac tctcacctag atataaccca tgtgtgtgtt tccaatcatc
     9661 aatcgcttag gcgaaaatgg gaaggttagc gtagacaccg ggatcgttga atcgcaggtt
     9721 cttgttgagg ttggagatct ccttaatctc ggcctcggtc aggtcaaagt ccagagattc
     9781 gaggttctga gtttgtcgct cgacgttgtt ggacttgggg atgacggcaa tgcctcgctg
     9841 ggtagcccat cgcagcagaa cctgggaggg ggtcttgttg tgagccttgg cgatcttggt
     9901 gatggtctcg ttctcgaaaa gaggagggac cttctggtcc atgccaatct ccacaaagga
     9961 ggcggggccg aaagaagagt aggcagtgac ctggatgttg ttgtcctttg cgagctgggt
    10021 cagctggggc tgcaccaggt aggggtggtg ctcaatctgc agagcagaaa tggggtgctt
    10081 gttgtaggtc tggacgtcgt aaatgttctg ggcggtgaag ttggagacac caatggactt
    10141 ggcgattccg gtatcaacta gctcctcgag agcctcccag gtctctcggg tagggatctt
    10201 ggccagaggg ttaggacccg agtcagtggc gttggtccat ccagcccact ccttctcgga
    10261 gatgggaatg tactgcatgg ggatggggaa gtggatgagg tagagatcga tgtagtcgag
    10321 tccccaccac tcgttctgct ccttggcgat ctgcagagcg tgctcctttg agtggaaggt
    10381 gttccacaac ttggtggtga tgaagatgtc ctctcgcttg accaggccat ccttgatggc
    10441 tcgtcggata ccttcgccgg cctctcgctc gttctggtag tcgaaggcgc cgtcaaacag
    10501 tctgtaaccg ttcttgatgg ctccgtagac ggtgtcggcg gtcttgtcac gggggacctt
    10561 ccacaggccg aatccgacct tgggcatgga ctttccggag gcgagcttga aggacattgt
    10621 aactgtggtg tgaatttctc cgcaggggta gttgatggag agaagaagag atggtgcagc
    10681 ggaatttttg gatatatggt tttttttttg gatatggtaa caaaaccaaa acactaaaag
    10741 caaacggtag gttgccttca aatagagttg cgtctaccca gtaatttatg gaccttttgc
    10801 gttgcgacaa tattgtcttg tacgacagag atatggacgg agcgggccag gtgaggggaa
    10861 aaaggaaagt cacgtgaccc actccgcccc cgagtctcgc cgttgttttt ctcctctttt
    10921 gcgggctcct gtttccgcgt cgcaatggtc ttgacttgtg ggggttgtag gcatgtatcc
    10981 gtagttagca cacccagctg ctacatgtag ccccgcacgg tcaacgtcat gttacttttt
    11041 cccgctaaaa aagttcacac gaatgttgag agctgtcaaa tcaactgggg cgtgttcaga
    11101 gcacgaggct ttgtgcgccg gagggagagc gtctgtggga gttatttgcc cgcatgccat
    11161 tggtctgtcc agctatcgtg actcatgata catatctgct tacaaggtct gccgtcacta
    11221 gacgataaaa ttgggagact gtaaaaaggt cttctagaag agctgccaga ggtggattga
    11281 tgaaggggga gattgatggc gcaaggggat agtagaaagg gcacggcgat gcactgaaat
    11341 gactaccgct tgtcaatact accaatatca cagatgtaca agtacacata tctaacccgc
    11401 tttgtttcca ctcgtacact ccattcatat ctatctccaa tcagcactct aagacctgta
    11461 tgtactgtag ttggaaaata ccaagccctg catgtactac ggtacaaaaa aaacggtaca
    11521 tacagtcagc gacatcggcc ttccaacaag acaaggagag gcgggaacaa tgtatgctgc
    11581 acaccacgga gactggttgc aaacaacaga agcgcgcaat cgc
//
