LOCUS       sequence-316922-        9659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing HRP-TM in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, 
            Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY
  TITLE     Proteomic Analysis of Unbounded Cellular Compartments: Synaptic 
            Clefts.
  JOURNAL   Cell. 2016 Aug 25;166(5):1295-1307.e21. doi: 
            10.1016/j.cell.2016.07.041.
  PUBMED    27565350
REFERENCE   2  (bases 1 to 9659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1295-1307.e21"
COMMENT     Sequence Label: FSW-HRP-TM
FEATURES             Location/Qualifiers
     source          1..9659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2399..2846
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      2860..2869
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     2866..2925
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             2929..2955
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3907..3936
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3940..4086
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    4114..4702
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4167..4187)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4585..4596)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4705..4721)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4706..4722)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5227..5407
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5433..5450)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5439..5663
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5709..6137
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5796..5815)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6006..6027
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6146..6166)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6151..6480
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6331..6466
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6393..6412
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6528..6575
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6594..6968
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7098..7219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7135..7154)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7189..7208
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7268..7284)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7268..7284)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7281..7303)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7292..7308
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7316..7322,7323..7340,7341..7346))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7361..7382
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7499..7516)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7670..8258)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7750..7769)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8429..9220,9221..9289))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9052..9071
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9290..9394)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9469..9488)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagc acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaataccgc catgcatctg cagagggccc tgcgtatgag
     2401 tgcaagtggg ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc
     2461 ccgacccact ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga
     2521 gggggagggg aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga
     2581 cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc
     2641 tgacgtcact cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg
     2701 ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg
     2761 accatctgcg ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag
     2821 gagtcgtgtc gtgcctgaga gcgcagtcta gaggatcccg ccaccatgga gacagacaca
     2881 ctcctgctat gggtactgct gctctgggtt ccaggttcca ctggtgacta tccatatgat
     2941 gttccagatt atgctggggc ccagcagtta acccctacat tctacgacaa tagctgtccc
     3001 aacgtgtcca acatcgttcg cgacacaatc gtcaacgagc tcagatccga tcccaggatc
     3061 gctgcttcaa tattacgtct gcacttccat gactgcttcg tgaatggttg cgacgctagc
     3121 atattactgg acaacaccac cagtttccgc actgaaaagg atgcattcgg gaacgctaac
     3181 agcgccaggg gctttccagt gatcgatcgc atgaaggctg ccgttgagtc agcatgccca
     3241 cgaacagtca gttgtgcaga cctgctgact atagctgcgc aacagagcgt gactcttgca
     3301 ggcggaccgt cctggagagt gccgctcggt cgacgtgact ccctacaggc attcctagat
     3361 ctggccaacg ccaacttgcc tgctccattc ttcaccctgc cccagctgaa ggatagcttt
     3421 agaaacgtgg gtctgaatcg ctcgagtgac cttgtggctc tgtccggagg acacacattt
     3481 ggaaagaacc agtgtaggtt catcatggat aggctctaca atttcagcaa cactgggtta
     3541 cctgacccca cgctgaacac tacgtatctc cagacactga gaggcttgtg cccactgaat
     3601 ggcaacctca gtgcactagt ggactttgat ctgcggaccc caaccatctt cgataacaag
     3661 tactatgtga atctagagga gcagaaaggc ctgatacaga gtgatcaaga actgtttagc
     3721 agtccaaacg ccactgacac catcccactg gtgagaagtt ttgctaactc tactcaaacc
     3781 ttctttaacg ccttcgtgga agccatggac cgtatgggta acattacccc tctgacgggt
     3841 acccaaggcc agattcgtct gaactgcaga gtggtcaaca gcaactctcc gcggctgcag
     3901 gtcgacgaac aaaaactcat ctcagaagag gatctgaatg ctgtgggcca ggacacgcag
     3961 gaggtcatcg tggtgccaca ctccttgccc tttaaggtgg tggtgatctc agccatcctg
     4021 gccctggtgg tgctcaccat catctccctt atcatcctca tcatgctttg gcagaagaag
     4081 ccacgttagg aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     4141 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4201 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4261 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4321 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4381 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4441 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4501 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     4561 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4621 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4681 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga
     4741 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     4801 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     4861 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     4921 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     4981 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     5041 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     5101 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     5161 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     5221 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5281 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5341 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5401 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     5461 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     5521 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     5581 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     5641 tggggatgcg gcgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     5701 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5761 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5821 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5881 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5941 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6001 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6061 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6121 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     6181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     6241 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6301 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6361 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     6421 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     6481 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
     6541 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     6601 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     6661 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     6721 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     6781 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     6841 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     6901 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     6961 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     7021 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     7081 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7141 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7201 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     7261 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7321 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7381 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7441 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7501 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7561 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7621 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7681 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7741 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7801 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7861 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7921 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7981 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8041 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8101 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8161 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8221 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8281 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8341 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8401 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8461 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8521 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8581 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8641 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8701 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8761 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8821 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8881 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8941 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9001 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9061 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9121 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9181 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9241 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9301 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9361 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9421 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     9481 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     9541 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     9601 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgtt
//
