LOCUS       sequence-316931-        9901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsXRN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Braun JE, Truffault V, Boland A, Huntzinger E, Chang CT, Haas G, 
            Weichenrieder O, Coles M, Izaurralde E
  TITLE     A direct interaction between DCP1 and XRN1 couples mRNA decapping to
            5' exonucleolytic degradation.
  JOURNAL   Nat Struct Mol Biol. 2012 Dec;19(12):1324-31. doi: 
            10.1038/nsmb.2413. Epub 2012 Nov 11.
  PUBMED    23142987
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2413";
            journalName: "Nat Struct Mol Biol"; date: "2012-12"; volume: "19"; 
            issue: "12"; pages: "1324-31"
COMMENT     Sequence Label: pT7-EGFP-C1-HsXRN1_K
FEATURES             Location/Qualifiers
     source          1..9901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     553..572
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        553..571
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      599..608
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(605..607,608..610,611..1321)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(605..607,608..610,611..1321)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(650..671)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(911..930)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1258..1279
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6629..6750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6666..6685)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6720..6739
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6757..7212)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6894..6915)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7106..7125
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7239..7343
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7345..7702
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7345..7536
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7368..7388)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7553..7688
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7737..8531
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7737..8531
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7791..7810)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8401..8420
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8719..8738)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8763..8810
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9139..9727
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9628..9647
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      541 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      601 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      661 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      721 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      781 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      841 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      901 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      961 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1021 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1081 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1141 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1201 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1261 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1321 gtccggactc agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggcat
     1381 gggagtcccc aagttttaca gatggatctc agagcggtat ccctgtctca gcgaagtggt
     1441 gaaagagcat cagattcctg aatttgacaa cttgtacctg gatatgaatg gaattataca
     1501 tcagtgctcc catcctaatg atgatgatgt tcactttaga atttcagatg ataaaatctt
     1561 tactgatatt tttcactacc tggaggtgtt gtttcgcatt attaaaccca ggaaagtgtt
     1621 ctttatggct gtagatggtg tggctcctcg agcaaaaatg aaccagcagc gtgggaggcg
     1681 ttttaggtca gcaaaggagg cagaagacaa aattaaaaag gcaatagaga agggagaaac
     1741 tcttcctaca gaggccagat ttgattccaa ctgtatcaca ccaggaactg aatttatggc
     1801 caggttacat gaacatctga agtattttgt aaatatgaaa atttccacag acaagtcatg
     1861 gcaaggagtt accatctact tctcaggcca tgagactcct ggagaaggag agcataaaat
     1921 catggaattt atcagatccg agaaagcaaa gccagatcat gatccaaaca ccagacactg
     1981 tctttatggt ttagatgctg acttgattat gcttggatta acaagtcatg aggcacattt
     2041 ttctctctta agagaagaag ttcgatttgg tggcaaaaaa acacaacggg tatgtgctcc
     2101 agaagaaact acatttcacc ttctacactt gtctttaatg agagagtata ttgactatga
     2161 gttttcagta ttaaaagaaa agatcacatt taaatatgat attgaaagga taatagatga
     2221 ttggattttg atggggtttc ttgttggtaa tgattttatc cctcatctac ctcatttaca
     2281 tattaatcat gatgcactgc ctcttcttta tggaacatat gttaccatcc tgccagaact
     2341 tgggggttat attaatgaaa gtgggcacct caacttacct cgatttgaga aataccttgt
     2401 gaaactatca gattttgatc gggagcactt cagtgaagtt tttgtggacc taaaatggtt
     2461 tgaaagcaaa gttggtaaca agtacctcaa tgaagcagca ggtgtcgcag cagaagaagc
     2521 caggaactac aaggaaaaga aaaagttaaa gggccaggaa aattctctgt gttggactgc
     2581 tttagacaaa aatgaaggcg aaatgataac ttctaaggat aatttagaag atgagactga
     2641 agatgatgac ctatttgaaa ctgagtttag acaatataaa agaacatatt acatgacgaa
     2701 gatgggggtt gacgtagtat ctgatgactt tctggctgat caagctgcat gttatgttca
     2761 ggcaatacag tggattttgc actattacta tcatggagtt cagtcctgga gctggtatta
     2821 tccttatcat tatgcacctt tcctgtctga tatacacaac atcagtacac tcaaaatcca
     2881 ttttgaacta ggaaaacctt ttaagccatt tgaacagctt cttgctgtac ttccagcagc
     2941 cagcaaaaat ttacttcctg catgctacca gcatttgatg accaatgaag actcaccaat
     3001 tatagaatat tacccacctg attttaaaac tgacctaaat gggaaacaac aggaatggga
     3061 agctgtggtg ttaatccctt ttattgatga gaagcgatta ttggaagcca tggagacatg
     3121 taaccactcc ctcaaaaagg aagagaggaa aagaaaccaa catagtgagt gcctaatgtg
     3181 ctggtatgat agagacacag agtttatcta tccttctcca tggccagaaa agttccctgc
     3241 catagaacga tgttgtacaa ggtataaaat aatatcctta gatgcttggc gtgtagacat
     3301 aaacaaaaac aaaataacca gaattgacca gaaagcatta tatttctgtg gatttcctac
     3361 tctgaaacac atcagacaca aatttttttt gaagaaaagt ggtgttcaag tattccagca
     3421 aagcagtcgt ggagaaaaca tgatgttgga aatcttagtg gatgcagaat cagatgaact
     3481 taccgtagaa aatgtagctt catcagtgct tggaaaatct gtctttgtta attggcctca
     3541 ccttgaggaa gctagagtcg tggctgtatc agatggagaa actaagtttt acttggaaga
     3601 acctccagga acacagaagc tttattcagg aagaactgcc ccaccatcta aagtggttca
     3661 tcttggagat aaagaacaat ctaactgggc aaaagaagta caaggaattt cagaacacta
     3721 cctgagaaga aaaggaataa taataaatga aacatctgca gttgtgtatg ctcagttact
     3781 cacaggtcgt aaatatcaaa taaatcaaaa tggtgaagtt cgtctagaga aacagtggtc
     3841 aaaacaagtt gttccttttg tttatcaaac tattgtcaag gacatccgag ctttcgactc
     3901 ccgtttctcc aatatcaaaa cattggatga tttgtttcct ctgagaagta tggtctttat
     3961 gctgggaact ccctattatg gctgcactgg agaagttcag gattcaggtg atgtgattac
     4021 agaaggtagg attcgtgtga ttttcagcat tccatgtgaa cccaatcttg atgctttaat
     4081 acagaaccag cataaatatt ctataaagta caacccagga tatgtgttgg ccagtcgcct
     4141 tggagtgagt ggataccttg tttcaaggtt tacaggaagt atttttattg gaagaggatc
     4201 taggagaaac cctcatggag accataaagc aaatgtgggt ttaaatctca aattcaacaa
     4261 gaaaaatgag gaggtacctg gatatactaa gaaagttgga agtgaatgga tgtattcatc
     4321 tgcagcagaa caacttctgg cagagtactt agagagagct ccagaactat ttagttatat
     4381 agccaaaaat agccaagagg atgtgttcta tgaagatgac atttggcctg gagaaaatga
     4441 gaatggtgct gaaaaagttc aagaaattat tacttggcta aaaggacatc ctgtcagtac
     4501 tttatctcgt tcttcttgtg atttacaaat tctggatgca gctattgttg agaaaattga
     4561 ggaagaagtc gaaaagtgca agcaaagaaa gaataataag aaggtgcgag taacagtgaa
     4621 accccatttg ctatacagac ctttagaaca gcaacatgga gtcattcctg atcgggatgc
     4681 agaattttgt ctttttgacc gtgttgtaaa tgtgagagaa aacttctcag ttccagttgg
     4741 ccttcgaggc accatcatag gaataaaagg agctaataga gaagccgatg tactatttga
     4801 agtattattt gatgaagaat ttcctggagg gttaacaata agatgctcac ctggtagagg
     4861 ttatcgactg ccaacaagtg ccttggtgaa cctttctcat gggagtcgct ctgaaactgg
     4921 aaatcagaag ttgacagcca tcgtaaaacc acaaccagct gtacatcaac atagctcaag
     4981 ttcatcagtt tcctctgggc atttgggagc cctcaaccat tcccctcaat cactttttgt
     5041 tcctactcaa gtacctacta aagatgatga tgaattctgc aacatttggc agtccttaca
     5101 gggatctgga aagatgcaat actttcagcc aactatacaa gagaagggtg cagttctacc
     5161 tcaagaaata agccaagtaa atcaacatca taaatctggc tttaatgaca acagtgttaa
     5221 atatcagcaa agaaaacatg accctcacag aaaatttaaa gaagagtgta agagtcctaa
     5281 agctgagtgt tggtcccaaa aaatgtccaa taagcagcct aactctggaa ttgagaactt
     5341 tttagcatct ttgaatatct ccaaagaaaa tgaagtacag tcatctcatc atggggagcc
     5401 tccaagtgaa gagcatttgt caccacagtc atttgccatg ggaacacgga tgcttaaaga
     5461 aattctaaaa attgatggct ctaacactgt ggaccataag aatgaaatca aacagattgc
     5521 taatgaaatc cctgtttcct ctaacagaag agatgaatat ggattaccct ctcagcctaa
     5581 acaaaataag aaattagcat cttatatgaa caagcctcac agtgctaatg agtaccataa
     5641 tgttcagtct atggacaata tgtgttggcc tgcccccagc cagatccctc ctgtatccac
     5701 accagtaact gaactttctc gaatttgttc ccttgttgga atgccacaac ctgatttctc
     5761 ctttcttagg atgccacaga caatgaccgt ttgccaagta aaattatcta atggcttact
     5821 ggtacatggg ccacagtgcc actctgaaaa tgaagccaaa gagaaagctg cactttttgc
     5881 tttacaacag ttgggctcct taggcatgaa tttccctttg ccttcacaag tatttgcaaa
     5941 ttatccttca gctgtaccac ctggaaccat tcctccagcc tttcccccac ctactggctg
     6001 ggatcactat ggaagcaact atgcattggg ggcagctaat ataatgcctt cgtcgtctca
     6061 tctctttggc tcaatgccat ggggaccatc ggtgccagtt cctgggaagc ccttccatca
     6121 tactttatat tctgggacca tgcccatggc tgggggaata ccagggggtg tgcacaatca
     6181 gtttatacct ctgcaggtta ctaaaaaaag ggttgcaaac aaaaagaact ttgagaataa
     6241 ggaagcccag agttctcaag ccactccagt tcagactagc cagccagatt cttccaacat
     6301 tgtcaaagta agtccacggg agagctcatc agcttctttg aagtcctctc cgattgctca
     6361 acctgcatct tcttttcaag ttgaaactgc ctctcaaggc catagtatat ctcaccataa
     6421 gtcaacacca atctcttctt caagaagaaa atcaagaaaa ctggctgtta attttggtgt
     6481 ttctaaacct tctgagtaag gatccaccgg atctagataa ctgatcataa tcagccatac
     6541 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     6601 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     6661 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6721 tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt
     6781 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     6841 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     6901 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     6961 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     7021 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     7081 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     7141 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     7201 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7261 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7321 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     7381 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7441 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7501 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7561 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7621 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7681 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     7741 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     7801 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     7861 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     7921 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     7981 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     8041 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     8101 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     8161 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     8221 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     8281 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     8341 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     8401 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     8461 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     8521 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     8581 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8641 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8701 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     8761 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     8821 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     8881 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     8941 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     9001 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9061 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9121 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9181 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9241 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9301 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9361 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9421 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9481 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9541 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9601 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9661 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9721 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9781 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     9841 ttagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     9901 g
//
