LOCUS       sequence-316933-        2731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term Myc 3C cleavable Kan resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 36 pCOLI_K_Myc
FEATURES             Location/Qualifiers
     source          1..2731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(117..932)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(117..932)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     840..859
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(933..1024)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1040..1789)
                     /direction=LEFT
                     /label=RSF ori
                     /note="Plasmids containing the RSF 1030 origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     complement(1050..1067)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1290..1309)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1985..2004
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1985..2003
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2041..2063
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2083..2100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2113..2142
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2149..2178
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2185..2214
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2221..2250
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2257..2286
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2293..2322
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2341..2364
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(2557..2575)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2571..2618
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gacaagctga cgaccgggtc tccgcaagtg gcacttttcg gggaaatgtg cgcggaaccc
       61 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag
      121 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
      181 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
      241 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
      301 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
      361 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
      421 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
      481 tgagcgagac gaaatacgcg gtcgctgtta aaaggacaat tacaaacagg aatcgaatgc
      541 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
      601 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
      661 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
      721 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
      781 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
      841 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta
      901 gagcaagacg tttcccgttg aatatggctc atactcttcc tttttcaata ttattgaagc
      961 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1021 caaataggca tgcagcgctc ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt
     1081 cgactgcggc gagcggtgtc agctcactca aaagcggtaa tacggttatc cacagaatca
     1141 ggggataaag ccggaaagaa catgtgagca aaaagcaaag caccggaaga agccaacgcc
     1201 gcaggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1261 ccagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1321 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1381 ttcgggaagc gtggcgcttt ctcatagctc acgctgttgg tatctcagtt cggtgtaggt
     1441 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1501 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1561 agccattggt aactgattta gaggactttg tcttgaagtt atgcacctgt taaggctaaa
     1621 ctgaaagaac agattttggt gagtgcggtc ctccaaccca cttaccttgg ttcaaagagt
     1681 tggtagctca gcgaaccttg agaaaaccac cgttggtagc ggtggttttt ctttatttat
     1741 gagatgatga atcaatcggt ctatcaagtc aacgaacagc tattccgtta ctctagattt
     1801 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
     1861 aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
     1921 caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt
     1981 gcgctaatac gactcactat agggagacca caacggtttc cctccggctc cggaaataat
     2041 tttgtttaac tttaagaagg agatatacat atgggaagtt ctcatcacca ccatcatcat
     2101 agttcgggct ccgagcagaa acttatctct gaggaagact taaatgggga gcagaagttg
     2161 atctcggagg aagatctgaa tggtgagcaa aagttaatct ccgaagaaga cttgaatggc
     2221 gaacaaaaac tgatctcaga ggaagatttg aacggcgaac agaagttaat ctctgaggag
     2281 gatctgaacg gagaacagaa acttatttcg gaagaagatt tgaacgggtc gagtggtagt
     2341 ctggaagttc tgttccaggg gcccggatcc cggtccgaca tatgagcgcg cggaattcaa
     2401 aggcctacgt cgacgagctc actagtcgcg gccgctttcg aatctagagc ctgcagtctc
     2461 gacaagcttg tcgagaagta ctagaggatg tacaacgcgt gcgcgctgat ccggctgcta
     2521 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
     2581 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaacctcag gcatttgaga
     2641 agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag
     2701 cggctattta acgaccctgc cctgaaccga c
//
