LOCUS       sequence-316934-        8290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsXRN1_1-1173.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Braun JE, Truffault V, Boland A, Huntzinger E, Chang CT, Haas G, 
            Weichenrieder O, Coles M, Izaurralde E
  TITLE     A direct interaction between DCP1 and XRN1 couples mRNA decapping to
            5' exonucleolytic degradation.
  JOURNAL   Nat Struct Mol Biol. 2012 Dec;19(12):1324-31. doi: 
            10.1038/nsmb.2413. Epub 2012 Nov 11.
  PUBMED    23142987
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2413";
            journalName: "Nat Struct Mol Biol"; date: "2012-12"; volume: "19"; 
            issue: "12"; pages: "1324-31"
COMMENT     Sequence Label: pT7-EGFP-C1-HsXRN1_1-1173_K
FEATURES             Location/Qualifiers
     source          1..8290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     553..572
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        553..571
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      599..608
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(605..607,608..610,611..1321)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(605..607,608..610,611..1321)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(650..671)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(911..930)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1258..1279
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5018..5139
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5055..5074)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5109..5128
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5146..5601)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5283..5304)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5495..5514
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5628..5732
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5734..6091
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5734..5925
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5757..5777)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5942..6077
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6126..6920
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6126..6920
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6180..6199)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6790..6809
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7108..7127)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7152..7199
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7528..8116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8017..8036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      541 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      601 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      661 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      721 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      781 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      841 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      901 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      961 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1021 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1081 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1141 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1201 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1261 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1321 gtccggactc agatctcgag ctcaagcttc gaattctgca gtcgacatgg gagtccccaa
     1381 gttttacaga tggatctcag agcggtatcc ctgtctcagc gaagtggtga aagagcatca
     1441 gattcctgaa tttgacaact tgtacctgga tatgaatgga attatacatc agtgctccca
     1501 tcctaatgat gatgatgttc actttagaat ttcagatgat aaaatcttta ctgatatttt
     1561 tcactacctg gaggtgttgt ttcgcattat taaacccagg aaagtgttct ttatggctgt
     1621 agatggtgtg gctcctcgag caaaaatgaa ccagcagcgt gggaggcgtt ttaggtcagc
     1681 aaaggaggca gaagacaaaa ttaaaaaggc aatagagaag ggagaaactc ttcctacaga
     1741 ggccagattt gattccaact gtatcacacc aggaactgaa tttatggcca ggttacatga
     1801 acatctgaag tattttgtaa atatgaaaat ttccacagac aagtcatggc aaggagttac
     1861 catctacttc tcaggccatg agactcctgg agaaggagag cataaaatca tggaatttat
     1921 cagatccgag aaagcaaagc cagatcatga tccaaacacc agacactgtc tttatggttt
     1981 agatgctgac ttgattatgc ttggattaac aagtcatgag gcacattttt ctctcttaag
     2041 agaagaagtt cgatttggtg gcaaaaaaac acaacgggta tgtgctccag aagaaactac
     2101 atttcacctt ctacacttgt ctttaatgag agagtatatt gactatgagt tttcagtatt
     2161 aaaagaaaag atcacattta aatatgatat tgaaaggata atagatgatt ggattttgat
     2221 ggggtttctt gttggtaatg attttatccc tcatctacct catttacata ttaatcatga
     2281 tgcactgcct cttctttatg gaacatatgt taccatcctg ccagaacttg ggggttatat
     2341 taatgaaagt gggcacctca acttacctcg atttgagaaa taccttgtga aactatcaga
     2401 ttttgatcgg gagcacttca gtgaagtttt tgtggaccta aaatggtttg aaagcaaagt
     2461 tggtaacaag tacctcaatg aagcagcagg tgtcgcagca gaagaagcca ggaactacaa
     2521 ggaaaagaaa aagttaaagg gccaggaaaa ttctctgtgt tggactgctt tagacaaaaa
     2581 tgaaggcgaa atgataactt ctaaggataa tttagaagat gagactgaag atgatgacct
     2641 atttgaaact gagtttagac aatataaaag aacatattac atgacgaaga tgggggttga
     2701 cgtagtatct gatgactttc tggctgatca agctgcatgt tatgttcagg caatacagtg
     2761 gattttgcac tattactatc atggagttca gtcctggagc tggtattatc cttatcatta
     2821 tgcgcctttc ctgtctgata tacacaacat cagtacactc aaaatccatt ttgaactagg
     2881 aaaacctttt aagccatttg aacagcttct tgctgtactt ccagcagcca gcaaaaattt
     2941 acttcctgca tgctaccagc atttgatgac caatgaagac tcaccaatta tagaatatta
     3001 cccacctgat tttaaaactg acctaaatgg gaaacaacag gaatgggaag ctgtggtgtt
     3061 aatccctttt attgatgaga agcgattatt ggaagccatg gagacatgta accactccct
     3121 caaaaaggaa gagaggaaaa gaaaccaaca tagtgagtgc ctaatgtgct ggtatgatag
     3181 agacacagag tttatctatc cttctccatg gccagaaaag ttccctgcca tagaacgatg
     3241 ttgtacaagg tataaaataa tatccttaga tgcttggcgt gtagacataa acaaaaacaa
     3301 aataaccaga attgaccaga aagcattata tttctgtgga tttcctactc tgaaacacat
     3361 cagacacaaa ttttttttga agaaaagtgg tgttcaagta ttccagcaaa gcagtcgtgg
     3421 agaaaacatg atgttggaaa tcttagtgga tgcagaatca gatgaactta ccgtagaaaa
     3481 tgtagcttca tcagtgcttg gaaaatctgt ctttgttaat tggcctcacc ttgaggaagc
     3541 tagagtcgtg gctgtatcag atggagaaac taagttttac ttggaagaac ctccaggaac
     3601 acagaagctt tattcaggaa gaactgcccc accatctaaa gtggttcatc ttggagataa
     3661 agaacaatct aactgggcaa aagaagtaca aggaatttca gaacactacc tgagaagaaa
     3721 aggaataata ataaatgaaa catctgcagt tgtgtatgct cagttactca caggtcgtaa
     3781 atatcaaata aatcaaaatg gtgaagttcg tctagagaaa cagtggtcaa aacaagttgt
     3841 tccttttgtt tatcaaacta ttgtcaagga catccgagct ttcgactccc gtttctccaa
     3901 tatcaaaaca ttggatgatt tgtttcctct gagaagtatg gtctttatgc tgggaactcc
     3961 ctattatggc tgcactggag aagttcagga ttcaggtgat gtgattacag aaggtaggat
     4021 tcgtgtgatt ttcagcattc catgtgaacc caatcttgat gctttaatac agaaccagca
     4081 taaatattct ataaagtaca acccaggata tgtgttggcc agtcgccttg gagtgagtgg
     4141 ataccttgtt tcaaggttta caggaagtat ttttattgga agaggatcta ggagaaaccc
     4201 tcatggagac cataaagcaa atgtgggttt aaatctcaaa ttcaacaaga aaaatgagga
     4261 ggtacctgga tatactaaga aagttggaag tgaatggatg tattcatctg cagcagaaca
     4321 acttctggca gagtacttag agagagctcc agaactattt agttatatag ccaaaaatag
     4381 ccaagaggat gtgttctatg aagatgacat ttggcctgga gaaaatgaga atggtgctga
     4441 aaaagttcaa gaaattatta cttggctaaa aggacatcct gtcagtactt tatctcgttc
     4501 ttcttgtgat ttacaaattc tggatgcagc tattgttgag aaaattgagg aagaagtcga
     4561 aaagtgcaag caaagaaaga ataataagaa ggtgcgagta acagtgaaac cccatttgct
     4621 atacagacct ttagaacagc aacatggagt cattcctgat cgggatgcag aattttgtct
     4681 ttttgaccgt gttgtaaatg tgagagaaaa cttctcagtt ccagttggcc ttcgaggcac
     4741 catcatagga ataaaaggag ctaatagaga agccgatgta ctatttgaag tattatttga
     4801 tgaagaattt cctggagggt taacaataag atgctcacct ggtagaggtt atcgactgcc
     4861 aacaagtgcc ttggtgaacc tttcttaagg atccaccgga tctagataac tgatcataat
     4921 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     4981 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     5041 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5101 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta
     5161 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     5221 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     5281 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     5341 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     5401 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     5461 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     5521 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     5581 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5641 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5701 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     5761 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5821 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     5881 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     5941 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6001 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     6061 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     6121 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     6181 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     6241 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     6301 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     6361 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     6421 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     6481 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     6541 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     6601 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     6661 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     6721 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     6781 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     6841 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     6901 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     6961 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7021 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7081 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     7141 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     7201 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     7261 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     7321 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     7381 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7441 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7501 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7561 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7621 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7681 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7741 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7801 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7861 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7921 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7981 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8041 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8101 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8161 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8221 cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     8281 tggagttccg
//
