LOCUS       sequence-316944-        2414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term 8xHis uncleavable Kan resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 42 pCOLI_K_C_HIS
FEATURES             Location/Qualifiers
     source          1..2414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..107)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(123..872)
                     /direction=LEFT
                     /label=RSF ori
                     /note="Plasmids containing the RSF 1030 origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     complement(133..150)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(373..392)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1068..1087
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1068..1086
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1124..1146
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1206..1229
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(1323..1341)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1337..1384
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(join(1614..2414,1..15))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(join(1614..2414,1..15))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2337..2356
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttgaatatgg ctcatactct tcctttttca atattattga agcatttatc agggttattg
       61 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gcatgcagcg
      121 ctcttccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcggt
      181 gtcagctcac tcaaaagcgg taatacggtt atccacagaa tcaggggata aagccggaaa
      241 gaacatgtga gcaaaaagca aagcaccgga agaagccaac gccgcaggcg tttttccata
      301 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagccagagg tggcgaaacc
      361 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      421 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      481 tttctcatag ctcacgctgt tggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      541 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      601 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccatt ggtaactgat
      661 ttagaggact ttgtcttgaa gttatgcacc tgttaaggct aaactgaaag aacagatttt
      721 ggtgagtgcg gtcctccaac ccacttacct tggttcaaag agttggtagc tcagcgaacc
      781 ttgagaaaac caccgttggt agcggtggtt tttctttatt tatgagatga tgaatcaatc
      841 ggtctatcaa gtcaacgaac agctattccg ttactctaga tttcagtgca atttatctct
      901 tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc atgttagtca
      961 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag
     1021 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctaa tacgactcac
     1081 tatagggaga ccacaacggt ttccctccgg ctccggaaat aattttgttt aactttaaga
     1141 aggagactgg atccgaatct agagcctgca gtctcgacaa gcttggaagc ggatctggat
     1201 ctggacatca tcatcatcat caccatcact aataagtcga gaagtactag aggatgtaca
     1261 acgcgtgcgc gctgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     1321 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     1381 tttgctgaaa cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa
     1441 accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac
     1501 aagctgacga ccgggtctcc gcaagtggca cttttcgggg aaatgtgcgc ggaaccccta
     1561 tttgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa
     1621 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     1681 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     1741 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     1801 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     1861 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
     1921 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     1981 gcgagacgaa atacgcggtc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     2041 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     2101 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     2161 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     2221 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     2281 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     2341 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag
     2401 caagacgttt cccg
//
