LOCUS       sequence-31697-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Egan SE, Giddings BW, Brooks MW, Buday L, Sizeland AM, Weinberg RA
  TITLE     Association of Sos Ras exchange protein with Grb2 is implicated in 
            tyrosine kinase signal transduction and transformation.
  JOURNAL   Nature. 1993 May 6. 363(6424):45-51.
  PUBMED    8479536
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1993 May 6. 363(6424):45-51."
COMMENT     Sequence Label: pSVE Grb2 myc 49L/203R
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             363..392
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          527..592
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             722..742
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tctctgtcaa gtttggaaac gatgtgcagc acttcaaggt gctccgagat ggagccggga
       61 agtacttcct ctgggtggtg aagttcaatt ctttgaatga gctggtggat tatcacagat
      121 ctacatctgt ctccagaaac cagcagatat tcctgcggga catagaacag gtgccacagc
      181 agccgacata cgtccaggcc ctctttgact ttgatcccca ggaggatgga gagctgggct
      241 tccgccgggg agattttatc catgtcatgg ataactcaga ccccaactgg tggaaaggag
      301 cttgccacgg gcagactcga atgtttcccc gcaattatgt cacccccgtg aaccggaacg
      361 tcgaacaaaa gcttatttct gaagaagact tgtgagtcga ctctagagcg gccgccaccg
      421 cggtggagct cgaattcatc gatggtacca gatctttgtg aaggaacctt acttctgtgg
      481 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
      541 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
      601 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
      661 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
      721 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcaga
//
