LOCUS       sequence-31698-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Egan SE, Giddings BW, Brooks MW, Buday L, Sizeland AM, Weinberg RA
  TITLE     Association of Sos Ras exchange protein with Grb2 is implicated in 
            tyrosine kinase signal transduction and transformation.
  JOURNAL   Nature. 1993 May 6. 363(6424):45-51.
  PUBMED    8479536
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1993 May 6. 363(6424):45-51."
COMMENT     Sequence Label: pSVE Grb2 myc 49L/203R
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(237..566)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      251..386
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     551..571
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(734..753)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctctggggat tttgccaaaa aaccacggat gtggtttcat ttctatgtag ttctttaaaa
       61 tgaagccgtc ttttccatta agctctgcct tgtaccagtt ctgatcacat tcttcgttca
      121 aaaccttgag gatgtccccc cttttgaagc tcagctcgtc gtctgcagta gctttgaagt
      181 catatttggc gatggcttcc attctgaggg atcctctaga gtcgacgatc gaagcttttg
      241 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag
      301 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
      361 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
      421 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
      481 ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
      541 gactttccac accctaactg acacacattc cacagctggc gtaatagcga agaggcccgc
      601 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
      661 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      721 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      781 ccccgtcaag ctctaaatcg ggggctccct ttaggg
//
