LOCUS       sequence-31699-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Dreosti E, Odermatt B, Dorostkar MM, Lagnado L
  TITLE     A genetically encoded reporter of synaptic activity in vivo.
  JOURNAL   Nat Methods. 2009 Dec . 6(12):883-9.
  PUBMED    19898484
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Dec . 6(12):883-9."
COMMENT     Sequence Label: I-SceI pB 5UAS::ZfSyGCaMP2
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(83..104)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(317..373)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      385..394
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(401..415,416..424))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(428..460)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(464..481)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gctcctcgcc cttgctcacc atgctccctc cggtaccgcc cttgtacagc tcgtccatgc
       61 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
      121 tggggtcttt cgaaagtttg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
      181 ggccgtcgcc gatgggggtg ttctgctggt agtggtaggc gagctgcacg ccgccgtcct
      241 cgatgttgtg gcggatcttg aagttcgcct tgatgccgtt cttctgcttg tcggccatga
      301 tatagacgtt ctcgagtgag ctcagccgac ctatagctct gactgcgtga cctgtcttat
      361 tccacttacg acgtgatgag tcgaccatgg tggcgagatc cttatcgtca tcgtcgtaca
      421 gatcccgacc catttgctgt ccaccagtca tgctagccat accatgatga tgatgatgat
      481 gagaaccccg catggtggcg accggtggat cctcgttgga gaaggatgtg ggctctccct
      541 gaccatacac tccttgattg tatccaccgc cttcatatcc accctgctgg ccgtagtcag
      601 gctgatatcc accctgagag ctggcatact ggtcctgtcc ataaccttcc tgtccatagc
      661 cctggccgaa ggagtctgga gctggctgct tctctccaga tggcatgtag gtgccagcga
      721 aagccgccat ccagccggtt tccttgaaga tgaaccagan gttgcctccc cataggatca
      781 cattacagaa cccgaatgcc acagatgtgt tcaggccaga caccaccggt tcgtgcagct
      841 ctttgcatcg gttttcctct cgttcgcacg cagggatgag
//
