LOCUS       sequence-317-exp        5056 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5056)
  TITLE     Fire Lab C. Elegans Vector Kit 2005
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD158.117
FEATURES             Location/Qualifiers
     source          1..5056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2630..2652)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2752..2771
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2849..2867)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2935..3039
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3040..3108,3109..3900)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3258..3277)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4071..4659
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4560..4579
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4813..4830
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4947..4968
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4983..4988,4989..5006,5007..5013)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5021..5037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5026..5048
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(5045..5056,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(5045..5056,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttcagtaaa agaagtagaa ttttatagtt ttttttctgt
       61 ttgaaaaatt ctccccatca atgttctttc aaataaatac atcactaatg caaagtattc
      121 tataacctca tatctaaatt cttcaaaatc ttaacatatc ttatcattgc tttaagtcaa
      181 acgtaacatt aaaaaaaatg ttttggaaaa tgtgtcaagt ctctcaaaat tcagtttttt
      241 aaaccactcc tatagtccta tagtcctata gttacccatg aaatccttat atattactgt
      301 aaaatgtttc aaaaaccatt ggcaaattgc cagaactgaa aatttccggc aaattgggga
      361 accggcaaat tgccaatttg ctgaatttgc cggaaaaacg gtaattgccg aaagtttttg
      421 acacgaaaat ggcaaattgt ggttttaaaa tttttttttt tggaaatttc agaatttcaa
      481 ttttaatcgg caaaactgta ggcatcctaa gaatgttcct acatctattt tgaaaagtaa
      541 gcgaattaat tctatgaaaa tgtctaaaga aaatggggaa acaatttcaa aaaggcacag
      601 tttcaagtgt ttccgaatta tactaaatcc ctctaaaaac ttccggcaaa ttgatatccg
      661 taaaagagca aatccgcatt tttgccgaaa attaaaattt ccgacaaatc ggcaaaccgg
      721 caatttggcg aaatttgccg gaaggcaatt gccgcccacc cctgttccag aggttcaaac
      781 tggtagcaaa gctcaaaatt tctcaaattc tccaattttt ttttgaattt tggcagtgta
      841 ccaaaatgac attcagtcat attggtttat tatagattta tttagataaa atcctaaatg
      901 attctacctt taaagatgcc cactttaaaa gtaatgactc aaacttcaaa ttgctctaag
      961 attctattga attaccatct tttcctctca ttttctctca ctgtctattt catcacaaat
     1021 tcatccctct ctccctctct ttctctctcc ctctctctct ttctctttgc tcatcatctg
     1081 tcattttgtc cgttcctctc tctgcgccct cagcgttccc cacactctct cgcttctctt
     1141 ttcctagacg tcttcttttt tcatcttctt cagccctttt tcgccatttt ccatctctgt
     1201 caatcattac ggacgacccc cattatcgat aagatctcca cggtggccgc gaattcctgc
     1261 agcccggtcg ccaccatgag gtcttccaag aatgttatca aggagttcat gaggtttaag
     1321 gttcgcatgg aaggaacggt caatgggcac gagtttgaaa tagaaggcga aggagagggg
     1381 aggccatacg aaggccacaa taccgtaaag cttaaggtaa ccaagggggg acctttgcca
     1441 tttgcttggg atattttgtc accacaattt cagtatggaa gcaaggtata tgtcaagcac
     1501 cctgccgaca taccagacta taaaaagctg tcatttcctg aaggatttgt aagtttaaac
     1561 ggggtgttac taactaacaa ataacattta aattttcaga aatgggaaag ggtcatgaac
     1621 tttgaagacg gtggcgtcgc tactgtcacc caagattcca gcttgcaaga tggctgtttc
     1681 atctacaagg tcaagttcat tggcgtgaac tttccttccg atggacctgt tatgcaaaag
     1741 aagacaatgg gctgggaagc cagcactgag cgtttgtatc ctcgtgatgg cgtgttgaaa
     1801 ggagagattc ataaggctct gaagctgaaa gacgggtaag tttaaactat atcttactaa
     1861 ctaactgtag agatttaaat tttcagtggt cattacctag ttgaattcaa aagtatttac
     1921 atggcaaaga agcctgtgca gctaccaggg tactactatg ttgataccaa actggatatc
     1981 acaagccaca acgaagacta tacaatcgtt gagcagtatg aaagaaccga gggacgccac
     2041 catctgttcc tttagcggcc gcgactctag aattccaact gagcgccggt cgctaccatt
     2101 accaacttgt ctggtgtcaa aaataatagg cctactagcc ggccatacaa gtaatccgga
     2161 tgatcgacgc caacgtcgtt gaattttcaa attttaaata ctgaatattt gttttttttc
     2221 ctattattta tttattctct ttgtgttttt tttcttgctt tctaaaaaat taattcaatc
     2281 caaatctaaa catttttttt tctctttccg tctcccaatt cgtattccgc tcctctcatc
     2341 tgaacacaat gtgcaagttt atttatcttc tcgctttcat ttcattagga cgtgggggga
     2401 attggtggaa gggggaaaca cacaaaagga tgatggaaat gaaataagga cacacaatat
     2461 gcaacaacat tcaattcaga aatatggagg aaggtttaaa agaaaacata aaaatatata
     2521 gaggaggaag gaaaactagt aaaaaataag caaagaaatt aggcgaacga tgagaattgt
     2581 cctcgcttgg gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     2641 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     2701 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     2761 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     2821 gaaaataccg catcaggcgg ccttaagggc ctcgtgatac gcctattttt ataggttaat
     2881 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     2941 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3001 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3061 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3121 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3181 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3241 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3301 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3361 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3421 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3481 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3541 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3601 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3661 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3721 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3781 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3841 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3901 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3961 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4021 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4081 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4141 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4201 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     4261 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4321 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4381 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4441 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4501 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4561 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4621 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4681 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4741 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4801 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     4861 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     4921 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     4981 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     5041 cacacaggaa acagct
//
