LOCUS       sequence-317007-        4174 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express N-terminal GFP tagged Dcp2 FL at endogenous level in 
            S.cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Xing W, Muhlrad D, Parker R, Rosen MK
  TITLE     A quantitative inventory of yeast P body proteins reveals principles
            of composition and specificity.
  JOURNAL   Elife. 2020 Jun 19;9. pii: 56525. doi: 10.7554/eLife.56525.
  PUBMED    32553117
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-06-19"; volume: "9. pii: 56525. doi"; pages: " 
            10.7554/eLife.56525"
COMMENT     Sequence Label: N-GFP-Dcp2
FEATURES             Location/Qualifiers
     source          1..4174
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             522..1235
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(552..580)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1175..1195
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gagctctcgt cgtaaggctg acactgcaga agaaaagtca taatttgaat actagccggt
       61 atgaaactgt gattgattaa cctggggtta cctaaagaga acataagtaa tactcatgac
      121 agaatcaaaa cacaatacaa aatttatccg aacctcggcc cgactgccgg ctcgccggga
      181 aaggggacaa ccgcttctat ccgtcgacta acttcatcgg cccaatggaa gctatgatat
      241 ggggatttcc attgagccga tagcaatgta gggtaatact gttgcgtata tagtgatagt
      301 tattgaattt tattaccctg cgggaatatt gagacatcac taagcacgaa ttttacgtct
      361 gaggaaagtt gaatgatggc aaaataacca ggaaaaacaa atattgaatc cttgtgaagg
      421 attccacagt tgtttaatcc tccttaagct cacttagtat caattgtcta aataatattg
      481 ctttgaatct gaaaaaaata aaagtacctt cgcattagac aatgagtaaa ggagaagaac
      541 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
      601 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
      661 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcacttatg
      721 gtgttcaatg cttttcaaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg
      781 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
      841 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
      901 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
      961 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
     1021 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     1081 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     1141 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     1201 ctgggattac acatggcatg gatgaactat acaaacggat ccccgggtta attaactcac
     1261 tgccgctacg acacgcattg gagaacgtta cttctgttga tagaatttta gaggacttat
     1321 tagtacgttt tattataaat tgtccgaatg aagatttatc gagtgtcgag agagagttat
     1381 ttcattttga agaagcctca tggttttaca cggatttcat caaattgatg aatccaactt
     1441 taccctccct aaagattaaa tcatttgctc aattgatcat aaaactatgt cctctggttt
     1501 ggaaatggga cataagagtg gatgaggcac tccagcaatt ctccaagtat aagaaaagta
     1561 taccggtgag gggcgctgcc atatttaacg agaacctgag taaaatttta ttggtacagg
     1621 gtactgaatc ggattcttgg tcattcccaa gggggaagat atctaaagat gaaaatgaca
     1681 tagattgttg cattagagaa gtgaaagaag aaattggttt cgatttgacg gactatattg
     1741 acgacaacca attcattgaa agaaatattc aaggtaaaaa ttacaaaata tttttgatat
     1801 ctggtgtttc agaagtcttc aattttaaac ctcaagttag aaatgaaatt gataagatag
     1861 aatggttcga ttttaagaaa atttctaaaa caatgtacaa atcaaatatc aagtattatc
     1921 tgattaattc catgatgaga cccttatcaa tgtggttaag gcatcagagg caaataaaaa
     1981 atgaagatca attgaaatcc tatgcggaag aacaattgaa attgttgttg ggtatcacta
     2041 aggaggagca gattgatccc ggtagagagt tgctgaatat gttacatact gcagtgcaag
     2101 ctaacagtaa taataatgcg gtctccaacg gacaggtacc ctcgagccaa gagcttcagc
     2161 atttgaaaga gcaatcagga gaacacaacc aacagaagga tcagcagtca tcgttttctt
     2221 ctcaacaaca accttcaata tttccatctc tttctgaacc gtttgctaac aataagaatg
     2281 ttataccacc tactatgcca atggctaacg tattcatgtc aaatcctcaa ttgtttgcga
     2341 caatgaatgg ccagcctttt gcacctttcc catttatgtt accattaact aacaatagta
     2401 atagcgctaa ccctattcca actccggtcc cccctaattt taatgctcct ccgaatccga
     2461 tggcttttgg tgttccaaac atgcataacc tttctggacc agcagtatct caaccgtttt
     2521 ccttgcctcc tgctccttta ccgagggact ctggttacag cagctcctcc cctgggcagt
     2581 tgttagatat actaaattcg aaaaagcctg acagcaacgt gcaatcaagc aaaaagccaa
     2641 agcttaaaat cttacagaga ggaacggact tgaattcaat caagcaaaac aataatgatg
     2701 aaactgctca ttcaaactct caagctttgc tagatttgtt gaaaaaacca acatcatcgc
     2761 agaagataca cgcttccaaa ccagatactt cctttttacc aaatgactcc gtatctggta
     2821 tacaagatgc agaatatgaa gatttcgaga gtagttcaga tgaagaggtg gagacagcta
     2881 gagatgaaag aaattcattg aatgtagata ttggggtgaa cgttatgcca agcgaaaaag
     2941 acagccgaag aagtcaaaag gaaaaaccaa ggaacgacgc aagcaaaaca aacttgaacg
     3001 cttctgcaga atctaatagt gtagaatggg ggcctggtaa atcttcaccc tcgactcaga
     3061 gtaaacagaa ttcatcagta ggaatgcaga ataaatatcg tcaagaaatt cacattggtg
     3121 atagtgatgc atatgaagtc ttcgaaagca gttctgacga agaagatgga aaaaaattgg
     3181 aagaactgga acaaactcaa gacaactcca aattaattag ccaagatatt ttaaaagaga
     3241 acaatttcca agacggtgaa gtgcctcata gagatatgcc cactgaaagt aataaatcta
     3301 taaacgaaac tgtcgggtta tcatcaacaa caaatactgt aaaaaaggta cccaaagtta
     3361 aaatactaaa acgaggtgaa acctttgcca gtctggcgaa tgataaaaaa gcttttgatt
     3421 caagctccaa tgtgtcttca tcaaaagatt tattacagat gctaagaaac ccaatttcct
     3481 ctactgtatc ttcaaatcag caatcaccaa aatctcaaca tctaagtggg gatgaagaaa
     3541 tcatgatgat gttgaagagg aattctgtgt caaaacctca aaatagcgaa gaaaatgcat
     3601 caacttcaag tataaacgat gcgaatgcaa gcgaactttt gggaatgtta aaacaaaagg
     3661 aaaaagacat tacagcacca aaacagccct ataatgttga ctcatactcg cagaaaaatt
     3721 cagctaaagg attgttgaat attttgaaaa agaacgactc taccgggtac cctcgcactg
     3781 agggtggacc gtcaagtgaa atgtcaacgt caatgaaacg caacgacgct acaaataatc
     3841 aagaattaga taagaattca acagagttat taaactactt aaagcccaag cctcttaatg
     3901 atgggtatga gaatatttca aataaggaca gttctcatga attactgaat atattgcatg
     3961 gtaataaaaa cagcagcgcc ttcaataata atgtatatgc aactgacgga tattccttgg
     4021 catctgataa caacgaaaat tcctcaaata aactactaaa tatgctgcaa aacaggtcta
     4081 gtgcaataaa tgagcccaac ttcgacgtcc gttcgaatgg aacttcaggg tctaatgaat
     4141 tattaagcat tttgcatagg aagtgaggcg cgcc
//
