LOCUS       sequence-31701-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Egan SE, Giddings BW, Brooks MW, Buday L, Sizeland AM, Weinberg RA
  TITLE     Association of Sos Ras exchange protein with Grb2 is implicated in 
            tyrosine kinase signal transduction and transformation.
  JOURNAL   Nature. 1993 May 6. 363(6424):45-51.
  PUBMED    8479536
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1993 May 6. 363(6424):45-51."
COMMENT     Sequence Label: pSVE Grb2 myc 49L
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             369..398
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          533..598
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             728..748
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tctccctctc tgtcaagttt ggaaacgatg tgcagcactt caaggtgctc cgagatggag
       61 ccgggaagta cttcctctgg gtggtgaagt tcaattcttt gaatgagctg gtggattatc
      121 acagatctac atctgtctcc agaaaccagc agatattcct gcgggacata gaacaggtgc
      181 cacagcagcc gacatacgtc caggccctct ttgactttga tccccaggag gatggagagc
      241 tgggcttccg ccggggagat tttatccatg tcatggataa ctcagacccc aactggtgga
      301 aaggagcttg ccacgggcag accggcatgt ttccccgcaa ttatgtcacc cccgtgaacc
      361 ggaacgtcga acaaaagctt atttctgaag aagacttgtg agtcgactct agagcggccg
      421 ccaccgcggt ggagctcgaa ttcatcgatg gtaccagatc tttgtgaagg aaccttactt
      481 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
      541 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
      601 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
      661 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
      721 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
      781 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
      841 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
      901 tataagtagg cataacagtt ataatcataa catactg
//
