LOCUS       sequence-31702-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Egan SE, Giddings BW, Brooks MW, Buday L, Sizeland AM, Weinberg RA
  TITLE     Association of Sos Ras exchange protein with Grb2 is implicated in 
            tyrosine kinase signal transduction and transformation.
  JOURNAL   Nature. 1993 May 6. 363(6424):45-51.
  PUBMED    8479536
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1993 May 6. 363(6424):45-51."
COMMENT     Sequence Label: pSVE Grb2 myc 49L
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(235..564)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      249..384
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     549..569
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(732..751)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctggggattt tgccaaaaaa ccacggatgt ggtttcattt ctatgtagtt ctttaaaatg
       61 aagccgtctt ttccattaag ctctgccttg taccagttct gatcacattc ttcgttcaaa
      121 accttgagga tgtcccccct tttgaagctc agctcgtcgt ctgcagtagc tttgaagtca
      181 tatttggcga tggcttccat tctgagggat cctctagagt cgacgatcga agcttttgca
      241 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
      301 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
      361 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
      421 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
      481 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
      541 ctttccacac cctaactgac acacattcca cagctggcgt aatagcgaag aggcccgcac
      601 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
      661 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      721 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      781 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
      841 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
//
